Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus.
Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% of Western-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seiz...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4684321?pdf=render |
| _version_ | 1811191594088398848 |
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| author | Bart C Jongbloets Koen L I van Gassen Anne A Kan Anneke H O Olde Engberink Marina de Wit Inge G Wolterink-Donselaar Marian J A Groot Koerkamp Onno van Nieuwenhuizen Frank C P Holstege Pierre N E de Graan |
| author_facet | Bart C Jongbloets Koen L I van Gassen Anne A Kan Anneke H O Olde Engberink Marina de Wit Inge G Wolterink-Donselaar Marian J A Groot Koerkamp Onno van Nieuwenhuizen Frank C P Holstege Pierre N E de Graan |
| author_sort | Bart C Jongbloets |
| collection | DOAJ |
| description | Febrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% of Western-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsion-susceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice. We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS. We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice. We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short- and long-term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis. |
| first_indexed | 2024-04-11T23:39:42Z |
| format | Article |
| id | doaj.art-080854a2caa24cb6a62a803239215788 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T23:39:42Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-080854a2caa24cb6a62a8032392157882022-12-22T03:56:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014524710.1371/journal.pone.0145247Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus.Bart C JongbloetsKoen L I van GassenAnne A KanAnneke H O Olde EngberinkMarina de WitInge G Wolterink-DonselaarMarian J A Groot KoerkampOnno van NieuwenhuizenFrank C P HolstegePierre N E de GraanFebrile seizures are the most prevalent type of seizures among children up to 5 years of age (2-4% of Western-European children). Complex febrile seizures are associated with an increased risk to develop temporal lobe epilepsy. To investigate short- and long-term effects of experimental febrile seizures (eFS), we induced eFS in highly febrile convulsion-susceptible C57BL/6J mice at post-natal day 10 by exposure to hyperthermia (HT) and compared them to normotherm-exposed (NT) mice. We detected structural re-organization in the hippocampus 14 days after eFS. To identify molecular candidates, which entrain this structural re-organization, we investigated temporal changes in mRNA expression profiles eFS 1 hour to 56 days after eFS. We identified 931 regulated genes and profiled several candidates using in situ hybridization and histology at 3 and 14 days after eFS. This is the first study to report genome-wide transcriptome analysis after eFS in mice. We identify temporal regulation of multiple processes, such as stress-, immune- and inflammatory responses, glia activation, glutamate-glutamine cycle and myelination. Identification of the short- and long-term changes after eFS is important to elucidate the mechanisms contributing to epileptogenesis.http://europepmc.org/articles/PMC4684321?pdf=render |
| spellingShingle | Bart C Jongbloets Koen L I van Gassen Anne A Kan Anneke H O Olde Engberink Marina de Wit Inge G Wolterink-Donselaar Marian J A Groot Koerkamp Onno van Nieuwenhuizen Frank C P Holstege Pierre N E de Graan Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus. PLoS ONE |
| title | Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus. |
| title_full | Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus. |
| title_fullStr | Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus. |
| title_full_unstemmed | Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus. |
| title_short | Expression Profiling after Prolonged Experimental Febrile Seizures in Mice Suggests Structural Remodeling in the Hippocampus. |
| title_sort | expression profiling after prolonged experimental febrile seizures in mice suggests structural remodeling in the hippocampus |
| url | http://europepmc.org/articles/PMC4684321?pdf=render |
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