Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p
Abstract Background Increasing studies confirmed that abnormal lncRNAs expression play a critical role in cervical cancer (CC) development and progression. LncRNA TPT1-AS1, a novel lncRNA, its role and underlying mechanisms involved in CC remain largely unknown. Methods Colony formation, EdU and Tra...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
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| Online Access: | http://link.springer.com/article/10.1186/s13046-018-0846-8 |
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| author | Hui Jiang Guanqun Huang Nianzhang Zhao Ting Zhang Mengni Jiang Yueming He Xinke Zhou Xianhan Jiang |
| author_facet | Hui Jiang Guanqun Huang Nianzhang Zhao Ting Zhang Mengni Jiang Yueming He Xinke Zhou Xianhan Jiang |
| author_sort | Hui Jiang |
| collection | DOAJ |
| description | Abstract Background Increasing studies confirmed that abnormal lncRNAs expression play a critical role in cervical cancer (CC) development and progression. LncRNA TPT1-AS1, a novel lncRNA, its role and underlying mechanisms involved in CC remain largely unknown. Methods Colony formation, EdU and Transwell assays were used to determine colony formation, proliferation, migration and invasion in vitro. The subcutaneous tumor model and tail vein injection lung metastasis model were performed to check tumor growth and metastasis in vivo. Luciferase activity and RIP experiment were carried out to determine the interaction between miR-324-5p and TPT1-AS1. Results We demonstrated for the first time that TPT1-AS1 expression was up-regulated in CC tissues and cell lines. High TPT1-AS1 was significantly correlated with adverse prognostic characteristics and poor survival. TPT1-AS1 overexpression and knockdown experiments revealed that TPT1-AS1 promoted cell colony formation, proliferation, migration, invasion and EMT progression of CC cells in vitro and in vivo. The underlying mechanism indicated that TPT1-AS1 functioned as an endogenous sponge for miR-324-5p in CC cells. Gain- and loss- experiment confirmed that miR-324-5p inhibited cell colony formation, proliferation, migration, invasion and EMT progression of CC cells, and mediated the biological effects of TPT1-AS1. Further investigations confirmed that SP1 was a direct target of miR-324-5p and mediated the effects of TPT1-AS1 and miR-324-5p in CC. Conclusions We demonstrated for the first time that TPT1-AS1 as an oncogenic lncRNA in CC progression and as a potential target for CC cure. |
| first_indexed | 2024-12-11T14:34:37Z |
| format | Article |
| id | doaj.art-0809d0ba74d74c098830da2ebfce0181 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-11T14:34:37Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-0809d0ba74d74c098830da2ebfce01812022-12-22T01:02:13ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662018-07-0137111210.1186/s13046-018-0846-8Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5pHui Jiang0Guanqun Huang1Nianzhang Zhao2Ting Zhang3Mengni Jiang4Yueming He5Xinke Zhou6Xianhan Jiang7Department of Abdominal Oncology, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of General Surgery, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Anesthesia, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Gynaecology, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Gynaecology, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Gynaecology, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Abdominal Oncology, The Fifth Affiliated Hospital of Guangzhou Medical UniversityDepartment of Abdominal Oncology, The Fifth Affiliated Hospital of Guangzhou Medical UniversityAbstract Background Increasing studies confirmed that abnormal lncRNAs expression play a critical role in cervical cancer (CC) development and progression. LncRNA TPT1-AS1, a novel lncRNA, its role and underlying mechanisms involved in CC remain largely unknown. Methods Colony formation, EdU and Transwell assays were used to determine colony formation, proliferation, migration and invasion in vitro. The subcutaneous tumor model and tail vein injection lung metastasis model were performed to check tumor growth and metastasis in vivo. Luciferase activity and RIP experiment were carried out to determine the interaction between miR-324-5p and TPT1-AS1. Results We demonstrated for the first time that TPT1-AS1 expression was up-regulated in CC tissues and cell lines. High TPT1-AS1 was significantly correlated with adverse prognostic characteristics and poor survival. TPT1-AS1 overexpression and knockdown experiments revealed that TPT1-AS1 promoted cell colony formation, proliferation, migration, invasion and EMT progression of CC cells in vitro and in vivo. The underlying mechanism indicated that TPT1-AS1 functioned as an endogenous sponge for miR-324-5p in CC cells. Gain- and loss- experiment confirmed that miR-324-5p inhibited cell colony formation, proliferation, migration, invasion and EMT progression of CC cells, and mediated the biological effects of TPT1-AS1. Further investigations confirmed that SP1 was a direct target of miR-324-5p and mediated the effects of TPT1-AS1 and miR-324-5p in CC. Conclusions We demonstrated for the first time that TPT1-AS1 as an oncogenic lncRNA in CC progression and as a potential target for CC cure.http://link.springer.com/article/10.1186/s13046-018-0846-8TPT1-AS1Cervical cancermiR-324-5pSP1EMT |
| spellingShingle | Hui Jiang Guanqun Huang Nianzhang Zhao Ting Zhang Mengni Jiang Yueming He Xinke Zhou Xianhan Jiang Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p Journal of Experimental & Clinical Cancer Research TPT1-AS1 Cervical cancer miR-324-5p SP1 EMT |
| title | Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p |
| title_full | Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p |
| title_fullStr | Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p |
| title_full_unstemmed | Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p |
| title_short | Long non-coding RNA TPT1-AS1 promotes cell growth and metastasis in cervical cancer via acting AS a sponge for miR-324-5p |
| title_sort | long non coding rna tpt1 as1 promotes cell growth and metastasis in cervical cancer via acting as a sponge for mir 324 5p |
| topic | TPT1-AS1 Cervical cancer miR-324-5p SP1 EMT |
| url | http://link.springer.com/article/10.1186/s13046-018-0846-8 |
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