Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae)
<p>Abstract</p> <p>Background</p> <p><it>Camptotheca acuminata </it>is a major natural source of the terpenoid indole alkaloid camptothecin (CPT). At present, little is known about the cellular distribution of the biosynthesis of CPT, which would be useful k...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-04-01
|
| Series: | BMC Plant Biology |
| Online Access: | http://www.biomedcentral.com/1471-2229/10/69 |
| _version_ | 1819034407307051008 |
|---|---|
| author | Santamaria Anna Trainotti Livio Valletta Alessio Pasqua Gabriella |
| author_facet | Santamaria Anna Trainotti Livio Valletta Alessio Pasqua Gabriella |
| author_sort | Santamaria Anna |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Camptotheca acuminata </it>is a major natural source of the terpenoid indole alkaloid camptothecin (CPT). At present, little is known about the cellular distribution of the biosynthesis of CPT, which would be useful knowledge for developing new strategies and technologies for improving alkaloid production.</p> <p>Results</p> <p>The pattern of CPT accumulation was compared with the expression pattern of some genes involved in CPT biosynthesis in <it>C. acuminata </it>[i.e., <it>Ca-TDC1 </it>and <it>Ca-TDC2 </it>(encoding for tryptophan decarboxylase) and <it>Ca-HGO </it>(encoding for 10-hydroxygeraniol oxidoreductase)]. Both CPT accumulation and gene expression were investigated in plants at different degrees of development and in plantlets subjected to drought-stress. In all organs, CPT accumulation was detected in epidermal idioblasts, in some glandular trichomes, and in groups of idioblast cells localized in parenchyma tissues. Drought-stress caused an increase in CPT accumulation and in the number of glandular trichomes containing CPT, whereas no increase in epidermal or parenchymatous idioblasts was observed. In the leaf, <it>Ca-TDC1 </it>expression was detected in some epidermal cells and in groups of mesophyll cells but not in glandular trichomes; in the stem, it was observed in parenchyma cells of the vascular tissue; in the root, no expression was detected. <it>Ca-TDC2 </it>expression was observed exclusively in leaves of plantlets subjected to drought-stress, in the same sites described for <it>Ca-TDC1</it>. In the leaf, <it>Ca-HGO </it>was detected in all chlorenchyma cells; in the stem, it was observed in the same sites described for <it>Ca-TDC1</it>; in the root, no expression was detected.</p> <p>Conclusions</p> <p>The finding that the sites of CPT accumulation are not consistently the same as those in which the studied genes are expressed demonstrates an organ-to-organ and cell-to-cell translocation of CPT or its precursors.</p> |
| first_indexed | 2024-12-21T07:33:14Z |
| format | Article |
| id | doaj.art-080b2440baaf431b9cc7fd1d894e7a23 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-21T07:33:14Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-080b2440baaf431b9cc7fd1d894e7a232022-12-21T19:11:31ZengBMCBMC Plant Biology1471-22292010-04-011016910.1186/1471-2229-10-69Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae)Santamaria AnnaTrainotti LivioValletta AlessioPasqua Gabriella<p>Abstract</p> <p>Background</p> <p><it>Camptotheca acuminata </it>is a major natural source of the terpenoid indole alkaloid camptothecin (CPT). At present, little is known about the cellular distribution of the biosynthesis of CPT, which would be useful knowledge for developing new strategies and technologies for improving alkaloid production.</p> <p>Results</p> <p>The pattern of CPT accumulation was compared with the expression pattern of some genes involved in CPT biosynthesis in <it>C. acuminata </it>[i.e., <it>Ca-TDC1 </it>and <it>Ca-TDC2 </it>(encoding for tryptophan decarboxylase) and <it>Ca-HGO </it>(encoding for 10-hydroxygeraniol oxidoreductase)]. Both CPT accumulation and gene expression were investigated in plants at different degrees of development and in plantlets subjected to drought-stress. In all organs, CPT accumulation was detected in epidermal idioblasts, in some glandular trichomes, and in groups of idioblast cells localized in parenchyma tissues. Drought-stress caused an increase in CPT accumulation and in the number of glandular trichomes containing CPT, whereas no increase in epidermal or parenchymatous idioblasts was observed. In the leaf, <it>Ca-TDC1 </it>expression was detected in some epidermal cells and in groups of mesophyll cells but not in glandular trichomes; in the stem, it was observed in parenchyma cells of the vascular tissue; in the root, no expression was detected. <it>Ca-TDC2 </it>expression was observed exclusively in leaves of plantlets subjected to drought-stress, in the same sites described for <it>Ca-TDC1</it>. In the leaf, <it>Ca-HGO </it>was detected in all chlorenchyma cells; in the stem, it was observed in the same sites described for <it>Ca-TDC1</it>; in the root, no expression was detected.</p> <p>Conclusions</p> <p>The finding that the sites of CPT accumulation are not consistently the same as those in which the studied genes are expressed demonstrates an organ-to-organ and cell-to-cell translocation of CPT or its precursors.</p>http://www.biomedcentral.com/1471-2229/10/69 |
| spellingShingle | Santamaria Anna Trainotti Livio Valletta Alessio Pasqua Gabriella Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae) BMC Plant Biology |
| title | Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae) |
| title_full | Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae) |
| title_fullStr | Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae) |
| title_full_unstemmed | Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae) |
| title_short | Cell-specific expression of tryptophan decarboxylase and 10-hydroxygeraniol oxidoreductase, key genes involved in camptothecin biosynthesis in <it>Camptotheca acuminata </it>Decne (Nyssaceae) |
| title_sort | cell specific expression of tryptophan decarboxylase and 10 hydroxygeraniol oxidoreductase key genes involved in camptothecin biosynthesis in it camptotheca acuminata it decne nyssaceae |
| url | http://www.biomedcentral.com/1471-2229/10/69 |
| work_keys_str_mv | AT santamariaanna cellspecificexpressionoftryptophandecarboxylaseand10hydroxygeranioloxidoreductasekeygenesinvolvedincamptothecinbiosynthesisinitcamptothecaacuminataitdecnenyssaceae AT trainottilivio cellspecificexpressionoftryptophandecarboxylaseand10hydroxygeranioloxidoreductasekeygenesinvolvedincamptothecinbiosynthesisinitcamptothecaacuminataitdecnenyssaceae AT vallettaalessio cellspecificexpressionoftryptophandecarboxylaseand10hydroxygeranioloxidoreductasekeygenesinvolvedincamptothecinbiosynthesisinitcamptothecaacuminataitdecnenyssaceae AT pasquagabriella cellspecificexpressionoftryptophandecarboxylaseand10hydroxygeranioloxidoreductasekeygenesinvolvedincamptothecinbiosynthesisinitcamptothecaacuminataitdecnenyssaceae |