Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic informati...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2021.632232/full |
| _version_ | 1818948408176541696 |
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| author | Dongyong Lv Yaohua Fan Wanhua Zhong Piyanuch Lonan Kunfeng Liu Maoyong Wu Yina Wu Yueliang Liang Xiaoping Lai Geng Li Liangwen Yu |
| author_facet | Dongyong Lv Yaohua Fan Wanhua Zhong Piyanuch Lonan Kunfeng Liu Maoyong Wu Yina Wu Yueliang Liang Xiaoping Lai Geng Li Liangwen Yu |
| author_sort | Dongyong Lv |
| collection | DOAJ |
| description | Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from Aerodramus fuciphagus and Aerodramus maximus, respectively. In addition, to distinguish different samples from different species of Apodiformes, we designed 4 polymerase chain reaction (PCR) amplification primers based on the ND2 gene sequences of A. fuciphagus and A. maximus. The ARMS-PCR results showed band lengths for A. fuciphagus EBN of 533, 402, and 201 bp, while those for A. maximus EBN were 463, 317, and 201 bp. Collectively, the results showed that ARMS-PCR is a fast and simple method for the genetic identification of EBN based on designing specific original identification primers. |
| first_indexed | 2024-12-20T08:46:19Z |
| format | Article |
| id | doaj.art-080b7edac4364d97a780c4cf3dfe73b2 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-20T08:46:19Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-080b7edac4364d97a780c4cf3dfe73b22022-12-21T19:46:14ZengFrontiers Media S.A.Frontiers in Genetics1664-80212021-03-011210.3389/fgene.2021.632232632232Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCRDongyong Lv0Yaohua Fan1Wanhua Zhong2Piyanuch Lonan3Kunfeng Liu4Maoyong Wu5Yina Wu6Yueliang Liang7Xiaoping Lai8Geng Li9Liangwen Yu10School of Nursing, Guangzhou University of Chinese Medicine, Guangzhou, ChinaShenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Guangzhou University of Chinese Medicine, Shenzhen, ChinaSchool of Physical Education and Health, Guangzhou University of Chinese Medicine, Guangzhou, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaSchool of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, ChinaGuangzhou Tongkang Pharmaceutical Co., Ltd., Guangzhou, ChinaGuangdong Provincial Key Laboratory of New Drug Development and Research of Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, ChinaLaboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, ChinaGuangdong Yunfu Vocational College of Chinese Medicine, Yunfu, ChinaEdible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from Aerodramus fuciphagus and Aerodramus maximus, respectively. In addition, to distinguish different samples from different species of Apodiformes, we designed 4 polymerase chain reaction (PCR) amplification primers based on the ND2 gene sequences of A. fuciphagus and A. maximus. The ARMS-PCR results showed band lengths for A. fuciphagus EBN of 533, 402, and 201 bp, while those for A. maximus EBN were 463, 317, and 201 bp. Collectively, the results showed that ARMS-PCR is a fast and simple method for the genetic identification of EBN based on designing specific original identification primers.https://www.frontiersin.org/articles/10.3389/fgene.2021.632232/fulledible bird’s nestARMS-PCRND2genetic informationguanidinium isothiocyanateAerodramus fuciphagus |
| spellingShingle | Dongyong Lv Yaohua Fan Wanhua Zhong Piyanuch Lonan Kunfeng Liu Maoyong Wu Yina Wu Yueliang Liang Xiaoping Lai Geng Li Liangwen Yu Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR Frontiers in Genetics edible bird’s nest ARMS-PCR ND2 genetic information guanidinium isothiocyanate Aerodramus fuciphagus |
| title | Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR |
| title_full | Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR |
| title_fullStr | Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR |
| title_full_unstemmed | Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR |
| title_short | Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR |
| title_sort | genetic identification of edible bird s nest in thailand based on arms pcr |
| topic | edible bird’s nest ARMS-PCR ND2 genetic information guanidinium isothiocyanate Aerodramus fuciphagus |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2021.632232/full |
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