Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats
Objective: Methotrexate (MTX) is known to have deleterious side effects on lung tissue. We aimed to investigate the effects of erythropoietin (EPO) and N-acetyl-cysteine (NAC) on MTX-induced lung injury in rats. Study Design: Animal experiment. Material and Methods: Twenty-six female Sprague-D...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Publishing House
2013-03-01
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| Series: | Balkan Medical Journal |
| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=431 |
| _version_ | 1797907759683862528 |
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| author | Hasan Kahraman Nurhan Köksal Ergül Kurutaş Betül Kabakcı Mahmut Tokur Selim Bozkurt Harun Çıralık Vedat Balkan |
| author_facet | Hasan Kahraman Nurhan Köksal Ergül Kurutaş Betül Kabakcı Mahmut Tokur Selim Bozkurt Harun Çıralık Vedat Balkan |
| author_sort | Hasan Kahraman |
| collection | DOAJ |
| description | Objective: Methotrexate (MTX) is known to have deleterious side effects on lung tissue. We aimed to investigate the effects of erythropoietin (EPO) and N-acetyl-cysteine (NAC) on MTX-induced lung injury in rats.
Study Design: Animal experiment.
Material and Methods: Twenty-six female Sprague-Dawley rats were divided into 4 groups. Sham group, 0.3 mL saline; MTX group, 5 mg/kg MTX; EPO group, 5mg/kg MTX and 2000 IU/kg EPO; NAC group, 5 mg/kg MTX and 200 mg/kg NAC were administered once daily for 4 consecutive days. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and inflammation and congestion scores in lung tissues were evaluated.
Results: In MTX group MDA were significantly higher, CAT and SOD were significantly lower than in sham, EPO and NAC groups (p<0.005). In EPO group MDA, CAT, and SOD were higher, but not significant than those in group NAC (p>0.005). In group MTX both scores were significantly higher than in sham (p<0.005). The congestion score of group MTX was significantly higher than those of group EPO and NAC (p<0.005).
Conclusion: EPO and NAC have significant preventive effects on MTX-induced lung injury in rats. Decreased antioxidant capacity and increased MDA level may cause the oxidative damage in MTX group. Also, higher antioxidant capacity and lower MDA level may be a response to oxidative stress in EPO and NAC groups. |
| first_indexed | 2024-04-10T10:41:47Z |
| format | Article |
| id | doaj.art-080c4a4ca9b54967806bf0496d34faad |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T10:41:47Z |
| publishDate | 2013-03-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-080c4a4ca9b54967806bf0496d34faad2023-02-15T16:20:29ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312013-03-013019910410.5152/balkanmedj.2012.078Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in RatsHasan Kahraman0Nurhan Köksal1Ergül Kurutaş2Betül Kabakcı3Mahmut Tokur4Selim Bozkurt5Harun Çıralık6Vedat Balkan7Department of Pulmonary, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Pulmonary, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Biochemistry, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Biochemistry, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Chest Surgery, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Emergency, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Pathology, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyDepartment of Pediatric Surgery, Faculty of Medicine, Kahramanmaraş Sütçü İmam University, Kahramanmaraş, TurkeyObjective: Methotrexate (MTX) is known to have deleterious side effects on lung tissue. We aimed to investigate the effects of erythropoietin (EPO) and N-acetyl-cysteine (NAC) on MTX-induced lung injury in rats. Study Design: Animal experiment. Material and Methods: Twenty-six female Sprague-Dawley rats were divided into 4 groups. Sham group, 0.3 mL saline; MTX group, 5 mg/kg MTX; EPO group, 5mg/kg MTX and 2000 IU/kg EPO; NAC group, 5 mg/kg MTX and 200 mg/kg NAC were administered once daily for 4 consecutive days. Malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and inflammation and congestion scores in lung tissues were evaluated. Results: In MTX group MDA were significantly higher, CAT and SOD were significantly lower than in sham, EPO and NAC groups (p<0.005). In EPO group MDA, CAT, and SOD were higher, but not significant than those in group NAC (p>0.005). In group MTX both scores were significantly higher than in sham (p<0.005). The congestion score of group MTX was significantly higher than those of group EPO and NAC (p<0.005). Conclusion: EPO and NAC have significant preventive effects on MTX-induced lung injury in rats. Decreased antioxidant capacity and increased MDA level may cause the oxidative damage in MTX group. Also, higher antioxidant capacity and lower MDA level may be a response to oxidative stress in EPO and NAC groups.http://balkanmedicaljournal.org/text.php?lang=en&id=431 |
| spellingShingle | Hasan Kahraman Nurhan Köksal Ergül Kurutaş Betül Kabakcı Mahmut Tokur Selim Bozkurt Harun Çıralık Vedat Balkan Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats Balkan Medical Journal |
| title | Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats |
| title_full | Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats |
| title_fullStr | Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats |
| title_full_unstemmed | Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats |
| title_short | Protective Effects of Erythropoietin and N-Acetylcysteine on Methotrexate-Induced Lung Injury in Rats |
| title_sort | protective effects of erythropoietin and n acetylcysteine on methotrexate induced lung injury in rats |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=431 |
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