Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection
Abstract Sepsis is life-threatening organ dysfunction and is considered a major cause of health loss. However, since the current biomarkers of sepsis reflect the host’s immune response to microorganisms, they would inevitably cause a time-lag. This means that there is still no truly reliable biomark...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-50864-0 |
| _version_ | 1797355764264730624 |
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| author | Akio Miyakoshi Hideki Niimi Tomohiro Ueno Masahiro Wakasugi Yoshitsugu Higashi Yuki Miyajima Masashi Mori Homare Tabata Hiroshi Minami Akinori Takaoka Atsushi Hayashi Yoshihiro Yamamoto Isao Kitajima |
| author_facet | Akio Miyakoshi Hideki Niimi Tomohiro Ueno Masahiro Wakasugi Yoshitsugu Higashi Yuki Miyajima Masashi Mori Homare Tabata Hiroshi Minami Akinori Takaoka Atsushi Hayashi Yoshihiro Yamamoto Isao Kitajima |
| author_sort | Akio Miyakoshi |
| collection | DOAJ |
| description | Abstract Sepsis is life-threatening organ dysfunction and is considered a major cause of health loss. However, since the current biomarkers of sepsis reflect the host’s immune response to microorganisms, they would inevitably cause a time-lag. This means that there is still no truly reliable biomarker of sepsis. In the present study, we developed a novel method for identifying and quantifying unknown pathogenic bacteria within four hours of sample collection. The most important point of this study is that the novel method can be used to determine the number of bacteria in a sample as a novel biomarker of infectious diseases. Indeed, based on the number of bacteria, we were able to accurately estimate the severity of microbial infection. Furthermore, using the time-dependent changes in the number of bacteria, we were able to monitor the therapeutic effect accurately. The rapid identification and quantification of bacteria may change our approach to medical care. |
| first_indexed | 2024-03-08T14:16:44Z |
| format | Article |
| id | doaj.art-080df6a060674ba4b2287656c06faa19 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T14:16:44Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-080df6a060674ba4b2287656c06faa192024-01-14T12:21:16ZengNature PortfolioScientific Reports2045-23222024-01-0114111010.1038/s41598-023-50864-0Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collectionAkio Miyakoshi0Hideki Niimi1Tomohiro Ueno2Masahiro Wakasugi3Yoshitsugu Higashi4Yuki Miyajima5Masashi Mori6Homare Tabata7Hiroshi Minami8Akinori Takaoka9Atsushi Hayashi10Yoshihiro Yamamoto11Isao Kitajima12Department of Ophthalmology, Toyama University HospitalClinical Laboratory Center, Toyama University HospitalClinical Laboratory Center, Toyama University HospitalDisaster and Emergency Center, Toyama University HospitalDepartment of Clinical Infectious Diseases, Toyama University HospitalDepartment of Clinical Infectious Diseases, Toyama University HospitalResearch Institute for Bioresources and Biotechnology, Ishikawa Prefectural UniversityLife Science Center, Hokkaido Mitsui Chemicals, Inc.Life Science Center, Hokkaido Mitsui Chemicals, Inc.Institute for Genetic Medicine, Hokkaido UniversityDepartment of Ophthalmology, Toyama University HospitalDepartment of Clinical Infectious Diseases, Toyama University HospitalAdministrative Office, University of ToyamaAbstract Sepsis is life-threatening organ dysfunction and is considered a major cause of health loss. However, since the current biomarkers of sepsis reflect the host’s immune response to microorganisms, they would inevitably cause a time-lag. This means that there is still no truly reliable biomarker of sepsis. In the present study, we developed a novel method for identifying and quantifying unknown pathogenic bacteria within four hours of sample collection. The most important point of this study is that the novel method can be used to determine the number of bacteria in a sample as a novel biomarker of infectious diseases. Indeed, based on the number of bacteria, we were able to accurately estimate the severity of microbial infection. Furthermore, using the time-dependent changes in the number of bacteria, we were able to monitor the therapeutic effect accurately. The rapid identification and quantification of bacteria may change our approach to medical care.https://doi.org/10.1038/s41598-023-50864-0 |
| spellingShingle | Akio Miyakoshi Hideki Niimi Tomohiro Ueno Masahiro Wakasugi Yoshitsugu Higashi Yuki Miyajima Masashi Mori Homare Tabata Hiroshi Minami Akinori Takaoka Atsushi Hayashi Yoshihiro Yamamoto Isao Kitajima Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection Scientific Reports |
| title | Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection |
| title_full | Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection |
| title_fullStr | Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection |
| title_full_unstemmed | Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection |
| title_short | Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection |
| title_sort | novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection |
| url | https://doi.org/10.1038/s41598-023-50864-0 |
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