Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells
Abstract Background Osteosarcoma is the most common primary malignant bone tumor in children and adolescents and has also been associated with a high degree of malignancy and enhanced metastatic capacity. Curcumin (CUR) is well known for its anti-osteosarcoma activity. However, both demethoxycurcumi...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-020-2857-1 |
| _version_ | 1818034241942847488 |
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| author | Cheng Huang Hsu-Feng Lu Yu-Hsuan Chen Jui-Chieh Chen Wen-Hsiang Chou Hsiu-Chen Huang |
| author_facet | Cheng Huang Hsu-Feng Lu Yu-Hsuan Chen Jui-Chieh Chen Wen-Hsiang Chou Hsiu-Chen Huang |
| author_sort | Cheng Huang |
| collection | DOAJ |
| description | Abstract Background Osteosarcoma is the most common primary malignant bone tumor in children and adolescents and has also been associated with a high degree of malignancy and enhanced metastatic capacity. Curcumin (CUR) is well known for its anti-osteosarcoma activity. However, both demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) are natural curcumin analogues/congeners from turmeric whose role in osteosarcoma development remains unknown. Methods To evaluate the growth inhibitory effects of CUR, DMC and BDMC on osteosarcoma (HOS and U2OS), breast (MDA-MB-231), and melanoma (A2058) cancer cells, we employed the MTT assay, annexin V-FITC /7-AAD staining, and clonogenic assay. Results CUR,DMC, and BDMC all decreased the viability of HOS, U2OS, MDA-MB-231, and A2058 cancer cells. Additionally, CUR,DMC, and BDMC induced the apoptosis of HOS cells through activation of Smad 2/3 or repression of Akt signaling pathway. Furthermore, the combination of CUR,DMC, and BDMC synergistically reduced cell viability, colony formation and increased apoptosis than either two or a single agent in HOS cells. Conclusions The combination of these three compounds could be used as a novel target for the treatment of osteosarcoma. |
| first_indexed | 2024-12-10T06:36:02Z |
| format | Article |
| id | doaj.art-0811f3c8248044b294bf06f7d2e2ae21 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-10T06:36:02Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-0811f3c8248044b294bf06f7d2e2ae212022-12-22T01:58:54ZengBMCBMC Complementary Medicine and Therapies2662-76712020-03-0120111110.1186/s12906-020-2857-1Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cellsCheng Huang0Hsu-Feng Lu1Yu-Hsuan Chen2Jui-Chieh Chen3Wen-Hsiang Chou4Hsiu-Chen Huang5Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming UniversityDepartments of Clinical Pathology, Cheng Hsin General HospitalDepartment of Applied Science, National Tsing Hua University South CampusDepartment of Biochemical Science and Technology, National Chiayi UniversitySchool of Medicine, National Defense Medical CenterDepartment of Applied Science, National Tsing Hua University South CampusAbstract Background Osteosarcoma is the most common primary malignant bone tumor in children and adolescents and has also been associated with a high degree of malignancy and enhanced metastatic capacity. Curcumin (CUR) is well known for its anti-osteosarcoma activity. However, both demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) are natural curcumin analogues/congeners from turmeric whose role in osteosarcoma development remains unknown. Methods To evaluate the growth inhibitory effects of CUR, DMC and BDMC on osteosarcoma (HOS and U2OS), breast (MDA-MB-231), and melanoma (A2058) cancer cells, we employed the MTT assay, annexin V-FITC /7-AAD staining, and clonogenic assay. Results CUR,DMC, and BDMC all decreased the viability of HOS, U2OS, MDA-MB-231, and A2058 cancer cells. Additionally, CUR,DMC, and BDMC induced the apoptosis of HOS cells through activation of Smad 2/3 or repression of Akt signaling pathway. Furthermore, the combination of CUR,DMC, and BDMC synergistically reduced cell viability, colony formation and increased apoptosis than either two or a single agent in HOS cells. Conclusions The combination of these three compounds could be used as a novel target for the treatment of osteosarcoma.http://link.springer.com/article/10.1186/s12906-020-2857-1CurcuminDemethoxycurcuminBisdemethoxycurcuminApoptosisOsteosarcoma |
| spellingShingle | Cheng Huang Hsu-Feng Lu Yu-Hsuan Chen Jui-Chieh Chen Wen-Hsiang Chou Hsiu-Chen Huang Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells BMC Complementary Medicine and Therapies Curcumin Demethoxycurcumin Bisdemethoxycurcumin Apoptosis Osteosarcoma |
| title | Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells |
| title_full | Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells |
| title_fullStr | Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells |
| title_full_unstemmed | Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells |
| title_short | Curcumin, demethoxycurcumin, and bisdemethoxycurcumin induced caspase-dependent and –independent apoptosis via Smad or Akt signaling pathways in HOS cells |
| title_sort | curcumin demethoxycurcumin and bisdemethoxycurcumin induced caspase dependent and independent apoptosis via smad or akt signaling pathways in hos cells |
| topic | Curcumin Demethoxycurcumin Bisdemethoxycurcumin Apoptosis Osteosarcoma |
| url | http://link.springer.com/article/10.1186/s12906-020-2857-1 |
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