Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract
Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the...
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| Materiálatiipa: | Artihkal |
| Giella: | English |
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Universitas Indonesia
2019-08-01
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| Ráidu: | Makara Journal of Health Research |
| Fáttát: | |
| Liŋkkat: | https://scholarhub.ui.ac.id/mjhr/vol23/iss2/10/ |
| _version_ | 1828003458126446592 |
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| author | Widya Lestari Wan N A W Yusry Siti H. Iskandar Solachuddin J A Ichwan Nining I. Irfan Wastuti H. Suriyah |
| author_facet | Widya Lestari Wan N A W Yusry Siti H. Iskandar Solachuddin J A Ichwan Nining I. Irfan Wastuti H. Suriyah |
| author_sort | Widya Lestari |
| collection | DOAJ |
| description | Background: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2. Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathways |
| first_indexed | 2024-04-10T06:57:49Z |
| format | Article |
| id | doaj.art-081303edcab345a0a24925bdd37c2d6a |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-04-10T06:57:49Z |
| publishDate | 2019-08-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-081303edcab345a0a24925bdd37c2d6a2023-02-28T04:30:50ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562019-08-0123212112610.7454/msk.v23i2.10632Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extractWidya Lestari0Wan N A W Yusry1Siti H. Iskandar2Solachuddin J A Ichwan3Nining I. Irfan4Wastuti H. Suriyah5Kulliyyah of Dentistry, International Islamic University Malaysia, MalaysiaKulliyyah of Dentistry, International Islamic University Malaysia, MalaysiaKulliyyah of Dentistry, International Islamic University Malaysia, MalaysiaKulliyyah of Dentistry, International Islamic University Malaysia, MalaysiaInternational Institute of Halal Research and Training, International Islamic University Malaysia, MalaysiaKulliyyah of Pharmacy, International Islamic University Malaysia, MalaysiaBackground: Myrmecodia pendans (M. pendans), or Sarang Semut, is an epiphyte with anticancer potential. It was recently reported that it induces apoptotic activity in the human oral squamous carcinoma (HSC-3) cell line. This study aimed to investigate the effect of M. pendans treated samples on the expression of apoptotic markers, Bax and Bcl-2. Methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was dried thoroughly and then extracted aqueously. The apoptotic activity was detected via flow cytometry. Bax and Bcl-2 expression was analyzed by quantitative real-time polymerase chain reaction. Results: Results of our cell cycle analysis reveal that aqueous extract of M. pendans induced apoptosis in 2.5 and 5 mg/mL but no change between these two concentrations. Apoptosis was observed at 24 h but not at 48 h. Bax and Bcl-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, upregulation of Bax was observed at 2.5 mg/mL. However, after 48 h, Bax expression showed no changes at any concentration. Bcl-2 was significantly downregulated after 48 h of treatment. Conclusions: M. pendans extract induced apoptosis in HSC-3 cells, which might occur via the proapoptotic (Bax) and antiapoptotic (Bcl-2) pathwayshttps://scholarhub.ui.ac.id/mjhr/vol23/iss2/10/apoptosiscell cycleflow cytometryreal-time polymerase chain reaction |
| spellingShingle | Widya Lestari Wan N A W Yusry Siti H. Iskandar Solachuddin J A Ichwan Nining I. Irfan Wastuti H. Suriyah Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract Makara Journal of Health Research apoptosis cell cycle flow cytometry real-time polymerase chain reaction |
| title | Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_full | Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_fullStr | Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_full_unstemmed | Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_short | Gene expression of selected apoptotic markers in human oral squamous carcinoma HSC-3 cell line treated with Myrmecodia pendans plant extract |
| title_sort | gene expression of selected apoptotic markers in human oral squamous carcinoma hsc 3 cell line treated with myrmecodia pendans plant extract |
| topic | apoptosis cell cycle flow cytometry real-time polymerase chain reaction |
| url | https://scholarhub.ui.ac.id/mjhr/vol23/iss2/10/ |
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