The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body
Defects in how excess nutrients are stored as triglycerides can result in several diseases including obesity, heart disease, and diabetes. Understanding the genes responsible for normal lipid homeostasis will help understand the pathogenesis of these diseases. RNAi screens performed in <i>Dros...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Medical Sciences |
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| Online Access: | https://www.mdpi.com/2076-3271/11/1/5 |
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| author | Austin M. Fruin Kelly E. Leon Justin R. DiAngelo |
| author_facet | Austin M. Fruin Kelly E. Leon Justin R. DiAngelo |
| author_sort | Austin M. Fruin |
| collection | DOAJ |
| description | Defects in how excess nutrients are stored as triglycerides can result in several diseases including obesity, heart disease, and diabetes. Understanding the genes responsible for normal lipid homeostasis will help understand the pathogenesis of these diseases. RNAi screens performed in <i>Drosophila</i> cells identified genes involved in vesicle formation and protein sorting as important for the formation of lipid droplets; however, all of the vesicular trafficking proteins that regulate lipid storage are unknown. Here, we characterize the function of the <i>Drosophila</i> Charged multivesicular protein 1 (<i>Chmp1</i>) gene in regulating fat storage. <i>Chmp1</i> is a member of the ESCRT-III complex that targets membrane localized signaling receptors to intralumenal vesicles in the multivesicular body of the endosome and then ultimately to the lysosome for degradation. When <i>Chmp1</i> levels are decreased specifically in the fly fat body, triglyceride accumulates while fat-body-specific <i>Chmp1</i> overexpression decreases triglycerides. <i>Chmp1</i> controls triglyceride storage by regulating the number and size of fat body cells produced and not by altering food consumption or lipid metabolic enzyme gene expression. Together, these data uncover a novel function for <i>Chmp1</i> in controlling lipid storage in <i>Drosophila</i> and supports the role of the endomembrane system in regulating metabolic homeostasis. |
| first_indexed | 2024-03-11T06:11:58Z |
| format | Article |
| id | doaj.art-0814e91c6c474d60b0975a5e4aeece16 |
| institution | Directory Open Access Journal |
| issn | 2076-3271 |
| language | English |
| last_indexed | 2024-03-11T06:11:58Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medical Sciences |
| spelling | doaj.art-0814e91c6c474d60b0975a5e4aeece162023-11-17T12:33:40ZengMDPI AGMedical Sciences2076-32712022-12-01111510.3390/medsci11010005The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat BodyAustin M. Fruin0Kelly E. Leon1Justin R. DiAngelo2Division of Science, Pennsylvania State University, Berks Campus, Reading, PA 19610, USADivision of Science, Pennsylvania State University, Berks Campus, Reading, PA 19610, USADivision of Science, Pennsylvania State University, Berks Campus, Reading, PA 19610, USADefects in how excess nutrients are stored as triglycerides can result in several diseases including obesity, heart disease, and diabetes. Understanding the genes responsible for normal lipid homeostasis will help understand the pathogenesis of these diseases. RNAi screens performed in <i>Drosophila</i> cells identified genes involved in vesicle formation and protein sorting as important for the formation of lipid droplets; however, all of the vesicular trafficking proteins that regulate lipid storage are unknown. Here, we characterize the function of the <i>Drosophila</i> Charged multivesicular protein 1 (<i>Chmp1</i>) gene in regulating fat storage. <i>Chmp1</i> is a member of the ESCRT-III complex that targets membrane localized signaling receptors to intralumenal vesicles in the multivesicular body of the endosome and then ultimately to the lysosome for degradation. When <i>Chmp1</i> levels are decreased specifically in the fly fat body, triglyceride accumulates while fat-body-specific <i>Chmp1</i> overexpression decreases triglycerides. <i>Chmp1</i> controls triglyceride storage by regulating the number and size of fat body cells produced and not by altering food consumption or lipid metabolic enzyme gene expression. Together, these data uncover a novel function for <i>Chmp1</i> in controlling lipid storage in <i>Drosophila</i> and supports the role of the endomembrane system in regulating metabolic homeostasis.https://www.mdpi.com/2076-3271/11/1/5triglyceride<i>Drosophila</i><i>Chmp1</i>fat body |
| spellingShingle | Austin M. Fruin Kelly E. Leon Justin R. DiAngelo The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body Medical Sciences triglyceride <i>Drosophila</i> <i>Chmp1</i> fat body |
| title | The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body |
| title_full | The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body |
| title_fullStr | The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body |
| title_full_unstemmed | The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body |
| title_short | The ESCRT-III Protein <i>Chmp1</i> Regulates Lipid Storage in the <i>Drosophila</i> Fat Body |
| title_sort | escrt iii protein i chmp1 i regulates lipid storage in the i drosophila i fat body |
| topic | triglyceride <i>Drosophila</i> <i>Chmp1</i> fat body |
| url | https://www.mdpi.com/2076-3271/11/1/5 |
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