SnoN facilitates axonal regeneration after spinal cord injury.
Adult CNS neurons exhibit a reduced capacity for growth compared to developing neurons, due in part to downregulation of growth-associated genes as development is completed. We tested the hypothesis that SnoN, an embryonically regulated transcription factor that specifies growth of the axonal compar...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3732222?pdf=render |
| _version_ | 1819231488972947456 |
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| author | Jiun L Do Azad Bonni Mark H Tuszynski |
| author_facet | Jiun L Do Azad Bonni Mark H Tuszynski |
| author_sort | Jiun L Do |
| collection | DOAJ |
| description | Adult CNS neurons exhibit a reduced capacity for growth compared to developing neurons, due in part to downregulation of growth-associated genes as development is completed. We tested the hypothesis that SnoN, an embryonically regulated transcription factor that specifies growth of the axonal compartment, can enhance growth in injured adult neurons. In vitro, SnoN overexpression in dissociated adult DRG neuronal cultures significantly enhanced neurite outgrowth. Moreover, TGF-β1, a negative regulator of SnoN, inhibited neurite outgrowth, and SnoN over-expression overcame this inhibition. We then examined whether SnoN influenced axonal regeneration in vivo: indeed, expression of a mutant form of SnoN resistant to degradation significantly enhanced axonal regeneration following cervical spinal cord injury, despite peri-lesional upregulation of TGF-β1. Thus, a developmental mechanism that specifies extension of the axonal compartment also promotes axonal regeneration after adult CNS injury. |
| first_indexed | 2024-12-23T11:45:46Z |
| format | Article |
| id | doaj.art-0817300a011a4785ba3e3d5f4acf8a08 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:45:46Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0817300a011a4785ba3e3d5f4acf8a082022-12-21T17:48:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0188e7190610.1371/journal.pone.0071906SnoN facilitates axonal regeneration after spinal cord injury.Jiun L DoAzad BonniMark H TuszynskiAdult CNS neurons exhibit a reduced capacity for growth compared to developing neurons, due in part to downregulation of growth-associated genes as development is completed. We tested the hypothesis that SnoN, an embryonically regulated transcription factor that specifies growth of the axonal compartment, can enhance growth in injured adult neurons. In vitro, SnoN overexpression in dissociated adult DRG neuronal cultures significantly enhanced neurite outgrowth. Moreover, TGF-β1, a negative regulator of SnoN, inhibited neurite outgrowth, and SnoN over-expression overcame this inhibition. We then examined whether SnoN influenced axonal regeneration in vivo: indeed, expression of a mutant form of SnoN resistant to degradation significantly enhanced axonal regeneration following cervical spinal cord injury, despite peri-lesional upregulation of TGF-β1. Thus, a developmental mechanism that specifies extension of the axonal compartment also promotes axonal regeneration after adult CNS injury.http://europepmc.org/articles/PMC3732222?pdf=render |
| spellingShingle | Jiun L Do Azad Bonni Mark H Tuszynski SnoN facilitates axonal regeneration after spinal cord injury. PLoS ONE |
| title | SnoN facilitates axonal regeneration after spinal cord injury. |
| title_full | SnoN facilitates axonal regeneration after spinal cord injury. |
| title_fullStr | SnoN facilitates axonal regeneration after spinal cord injury. |
| title_full_unstemmed | SnoN facilitates axonal regeneration after spinal cord injury. |
| title_short | SnoN facilitates axonal regeneration after spinal cord injury. |
| title_sort | snon facilitates axonal regeneration after spinal cord injury |
| url | http://europepmc.org/articles/PMC3732222?pdf=render |
| work_keys_str_mv | AT jiunldo snonfacilitatesaxonalregenerationafterspinalcordinjury AT azadbonni snonfacilitatesaxonalregenerationafterspinalcordinjury AT markhtuszynski snonfacilitatesaxonalregenerationafterspinalcordinjury |