MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer
Background/Aims: To investigate the cellular effects and clinical significance of microRNA-223 (miR-223) in breast cancer by targeting stromal interaction molecule1 (STIM1). Methods: Breast cancer cell lines (T47D, MCF-7, SKB-R3, MDA-MB-231 and MDA-MB-435) and a normal breast epithelial cell line (M...
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| Format: | Article |
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Cell Physiol Biochem Press GmbH & Co KG
2018-01-01
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| Series: | Cellular Physiology and Biochemistry |
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| Online Access: | https://www.karger.com/Article/FullText/487180 |
| _version_ | 1819263018838523904 |
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| author | Yanfang Yang Zhansheng Jiang Ning Ma Bin Wang Jun Liu Lina Zhang Lin Gu |
| author_facet | Yanfang Yang Zhansheng Jiang Ning Ma Bin Wang Jun Liu Lina Zhang Lin Gu |
| author_sort | Yanfang Yang |
| collection | DOAJ |
| description | Background/Aims: To investigate the cellular effects and clinical significance of microRNA-223 (miR-223) in breast cancer by targeting stromal interaction molecule1 (STIM1). Methods: Breast cancer cell lines (T47D, MCF-7, SKB-R3, MDA-MB-231 and MDA-MB-435) and a normal breast epithelial cell line (MCF-10A) were prepared for this study. MiR-223 mimics, anti-miR-223 and pcDNA 3.1-STIM1 were transiently transfected into cancer cells independently or together, and then RT-qPCR was performed to detect the expressions of miR-223 and STIM1 mRNA, dual-luciferase reporter assay was conducted to examine the effects of miR-223 on STIM1, Western blotting was used to measure the expressions of the STIM1 proteins, MTT and Trans-well assays were performed to detect cell proliferation and invasion. Finally, the correlation of miR-223 and STIM1 was investigated by detecting with ISH and IHC in breast cancer specimens or the corresponding adjacent normal tissues. Results: Compared with normal cells and tissues, breast cancer tissues and cells exhibited significantly lower expression of miR-223, but higher expression of STIM1. MiR-223 could inhibit the proliferation and invasiveness of breast cancer cells by negatively regulating the expressions of STIM1. Reimplantation with STIM1 partially rescued the miRNA-223-induced inhibition of breast cancer cells. Clinical data revealed that high expression of STIM1 and miR-223 was respectively detrimental and beneficial factor impacting patient’s disease-free survival (DFS) rather than overall survival (OS). Moreover, Pearson correlation analysis also confirmed that STIM1 was inversely correlated with miR-223. Conclusion: MiR-223 inhibits the proliferation and invasion of breast cancer by targeting STIM1. The miR-223/STIM1 axis could possibly be a potential therapeutic target for treating breast cancer patients. |
| first_indexed | 2024-12-23T20:06:55Z |
| format | Article |
| id | doaj.art-08174d118c744fb39bf83674900c5e31 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-23T20:06:55Z |
| publishDate | 2018-01-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-08174d118c744fb39bf83674900c5e312022-12-21T17:32:54ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-01-0145285686610.1159/000487180487180MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast CancerYanfang YangZhansheng JiangNing MaBin WangJun LiuLina ZhangLin GuBackground/Aims: To investigate the cellular effects and clinical significance of microRNA-223 (miR-223) in breast cancer by targeting stromal interaction molecule1 (STIM1). Methods: Breast cancer cell lines (T47D, MCF-7, SKB-R3, MDA-MB-231 and MDA-MB-435) and a normal breast epithelial cell line (MCF-10A) were prepared for this study. MiR-223 mimics, anti-miR-223 and pcDNA 3.1-STIM1 were transiently transfected into cancer cells independently or together, and then RT-qPCR was performed to detect the expressions of miR-223 and STIM1 mRNA, dual-luciferase reporter assay was conducted to examine the effects of miR-223 on STIM1, Western blotting was used to measure the expressions of the STIM1 proteins, MTT and Trans-well assays were performed to detect cell proliferation and invasion. Finally, the correlation of miR-223 and STIM1 was investigated by detecting with ISH and IHC in breast cancer specimens or the corresponding adjacent normal tissues. Results: Compared with normal cells and tissues, breast cancer tissues and cells exhibited significantly lower expression of miR-223, but higher expression of STIM1. MiR-223 could inhibit the proliferation and invasiveness of breast cancer cells by negatively regulating the expressions of STIM1. Reimplantation with STIM1 partially rescued the miRNA-223-induced inhibition of breast cancer cells. Clinical data revealed that high expression of STIM1 and miR-223 was respectively detrimental and beneficial factor impacting patient’s disease-free survival (DFS) rather than overall survival (OS). Moreover, Pearson correlation analysis also confirmed that STIM1 was inversely correlated with miR-223. Conclusion: MiR-223 inhibits the proliferation and invasion of breast cancer by targeting STIM1. The miR-223/STIM1 axis could possibly be a potential therapeutic target for treating breast cancer patients.https://www.karger.com/Article/FullText/487180MicrornaMiR-223Stromal interaction molecule1Breast cancerProliferationInvasion |
| spellingShingle | Yanfang Yang Zhansheng Jiang Ning Ma Bin Wang Jun Liu Lina Zhang Lin Gu MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer Cellular Physiology and Biochemistry Microrna MiR-223 Stromal interaction molecule1 Breast cancer Proliferation Invasion |
| title | MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer |
| title_full | MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer |
| title_fullStr | MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer |
| title_full_unstemmed | MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer |
| title_short | MicroRNA-223 Targeting STIM1 Inhibits the Biological Behavior of Breast Cancer |
| title_sort | microrna 223 targeting stim1 inhibits the biological behavior of breast cancer |
| topic | Microrna MiR-223 Stromal interaction molecule1 Breast cancer Proliferation Invasion |
| url | https://www.karger.com/Article/FullText/487180 |
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