Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production
The mechanism of maintaining myometrial contractions during labor remains unclear. Autophagy has been reported to be activated in laboring myometrium, along with the high expression of Golgi reassembly stacking protein 2 (GORASP2), a protein capable of regulating autophagy activation. This study aim...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/10116 |
| _version_ | 1797594253428260864 |
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| author | Fan Yang Lina Chen Bolun Wen Xiaodi Wang Lele Wang Kaiyuan Ji Huishu Liu |
| author_facet | Fan Yang Lina Chen Bolun Wen Xiaodi Wang Lele Wang Kaiyuan Ji Huishu Liu |
| author_sort | Fan Yang |
| collection | DOAJ |
| description | The mechanism of maintaining myometrial contractions during labor remains unclear. Autophagy has been reported to be activated in laboring myometrium, along with the high expression of Golgi reassembly stacking protein 2 (GORASP2), a protein capable of regulating autophagy activation. This study aimed to investigate the role and mechanism of GORASP2 in uterine contractions during labor. Western blot confirmed the increased expression of GORASP2 in laboring myometrium. Furthermore, the knockdown of <i>GORASP2</i> in primary human myometrial smooth muscle cells (hMSMCs) using siRNA resulted in reduced cell contractility. This phenomenon was independent of the contraction-associated protein and autophagy. Differential mRNAs were analyzed using RNA sequencing. Subsequently, KEGG pathway analysis identified that <i>GORASP2</i> knockdown suppressed several energy metabolism pathways. Furthermore, reduced ATP levels and aerobic respiration impairment were observed in measuring the oxygen consumption rate (OCR). These findings suggest that GORASP2 is up-regulated in the myometrium during labor and modulates myometrial contractility mainly by maintaining ATP production. |
| first_indexed | 2024-03-11T02:20:58Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:20:58Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-081757eb1e424d6598d953f5381c670d2023-11-18T10:48:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124121011610.3390/ijms241210116Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP ProductionFan Yang0Lina Chen1Bolun Wen2Xiaodi Wang3Lele Wang4Kaiyuan Ji5Huishu Liu6School of Medicine, South China University of Technology, Guangzhou 510006, ChinaSchool of Medicine, South China University of Technology, Guangzhou 510006, ChinaGuangzhou Key Laboratory of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaGuangzhou Key Laboratory of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaGuangzhou Key Laboratory of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaGuangzhou Key Laboratory of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510623, ChinaSchool of Medicine, South China University of Technology, Guangzhou 510006, ChinaThe mechanism of maintaining myometrial contractions during labor remains unclear. Autophagy has been reported to be activated in laboring myometrium, along with the high expression of Golgi reassembly stacking protein 2 (GORASP2), a protein capable of regulating autophagy activation. This study aimed to investigate the role and mechanism of GORASP2 in uterine contractions during labor. Western blot confirmed the increased expression of GORASP2 in laboring myometrium. Furthermore, the knockdown of <i>GORASP2</i> in primary human myometrial smooth muscle cells (hMSMCs) using siRNA resulted in reduced cell contractility. This phenomenon was independent of the contraction-associated protein and autophagy. Differential mRNAs were analyzed using RNA sequencing. Subsequently, KEGG pathway analysis identified that <i>GORASP2</i> knockdown suppressed several energy metabolism pathways. Furthermore, reduced ATP levels and aerobic respiration impairment were observed in measuring the oxygen consumption rate (OCR). These findings suggest that GORASP2 is up-regulated in the myometrium during labor and modulates myometrial contractility mainly by maintaining ATP production.https://www.mdpi.com/1422-0067/24/12/10116labormyometrial contractility<i>GORASP2</i>ATP productionpregnancy |
| spellingShingle | Fan Yang Lina Chen Bolun Wen Xiaodi Wang Lele Wang Kaiyuan Ji Huishu Liu Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production International Journal of Molecular Sciences labor myometrial contractility <i>GORASP2</i> ATP production pregnancy |
| title | Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production |
| title_full | Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production |
| title_fullStr | Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production |
| title_full_unstemmed | Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production |
| title_short | Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production |
| title_sort | golgi reassembly stacking protein 2 modulates myometrial contractility during labor by affecting atp production |
| topic | labor myometrial contractility <i>GORASP2</i> ATP production pregnancy |
| url | https://www.mdpi.com/1422-0067/24/12/10116 |
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