Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves
Abstract Background In the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression. By using the replicating expression vector derived from bean yellow dwarf virus and green fluorescent protein as a repo...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-12-01
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| Series: | Bioresources and Bioprocessing |
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| Online Access: | https://doi.org/10.1186/s40643-021-00480-5 |
| _version_ | 1819009235023822848 |
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| author | Ying Li Min Sun Xin Wang Yue-Jing Zhang Xiao-Wei Da Ling-Yun Jia Hai-Long Pang Han-Qing Feng |
| author_facet | Ying Li Min Sun Xin Wang Yue-Jing Zhang Xiao-Wei Da Ling-Yun Jia Hai-Long Pang Han-Qing Feng |
| author_sort | Ying Li |
| collection | DOAJ |
| description | Abstract Background In the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression. By using the replicating expression vector derived from bean yellow dwarf virus and green fluorescent protein as a reporter, we investigated the effects of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine, as three common plant growth regulators, on the plant biomass and efficiency of transient expression during the process of transient expression in Nicotiana benthamiana L. leaves. Results With the increase of the concentration of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine (from 0.1 to 1.6 mg/L), the fresh weight, dry weight, and leaf area of the seedlings increased first and then returned to the levels similar to the controls (without chemical treatment). The treatment with α-naphthalene acetic acid at 0.2 and 0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.4 mg/L α-naphthalene acetic acid and was increased about by 19%, compared to the controls. Gibberellins3 at 0.1–0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.2 mg/L gibberellins3 and was increased by 25%. However, the application of 6-benzyladenine led to decrease in the level of transient expression of green fluorescent protein. Conclusions The appropriate plant growth regulators at moderate concentration could be beneficial to the expression of foreign genes from the Agrobacterium-mediated transient expression system in plants. Thus, appropriate plant growth regulators could be considered as exogenous components that are applied for the production of recombinant protein by plant-based transient expression systems. |
| first_indexed | 2024-12-21T00:53:08Z |
| format | Article |
| id | doaj.art-0817d00552734f9cb4115de6cf19517e |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-21T00:53:08Z |
| publishDate | 2021-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-0817d00552734f9cb4115de6cf19517e2022-12-21T19:21:21ZengSpringerOpenBioresources and Bioprocessing2197-43652021-12-01811810.1186/s40643-021-00480-5Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leavesYing Li0Min Sun1Xin Wang2Yue-Jing Zhang3Xiao-Wei Da4Ling-Yun Jia5Hai-Long Pang6Han-Qing Feng7School of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversitySchool of Life Sciences, Northwest Normal UniversityAbstract Background In the last decades, replicating expression vectors based on plant geminivirus have been widely used for enhancing the efficiency of plant transient expression. By using the replicating expression vector derived from bean yellow dwarf virus and green fluorescent protein as a reporter, we investigated the effects of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine, as three common plant growth regulators, on the plant biomass and efficiency of transient expression during the process of transient expression in Nicotiana benthamiana L. leaves. Results With the increase of the concentration of α-naphthalene acetic acid, gibberellins3, and 6-benzyladenine (from 0.1 to 1.6 mg/L), the fresh weight, dry weight, and leaf area of the seedlings increased first and then returned to the levels similar to the controls (without chemical treatment). The treatment with α-naphthalene acetic acid at 0.2 and 0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.4 mg/L α-naphthalene acetic acid and was increased about by 19%, compared to the controls. Gibberellins3 at 0.1–0.4 mg/L can enhance the level of transient expression of green fluorescent protein, which peaked at 0.2 mg/L gibberellins3 and was increased by 25%. However, the application of 6-benzyladenine led to decrease in the level of transient expression of green fluorescent protein. Conclusions The appropriate plant growth regulators at moderate concentration could be beneficial to the expression of foreign genes from the Agrobacterium-mediated transient expression system in plants. Thus, appropriate plant growth regulators could be considered as exogenous components that are applied for the production of recombinant protein by plant-based transient expression systems.https://doi.org/10.1186/s40643-021-00480-5Plant growth regulatorsAgrobacterium-mediated transformationGeminivirus-derived vectorTransient expression |
| spellingShingle | Ying Li Min Sun Xin Wang Yue-Jing Zhang Xiao-Wei Da Ling-Yun Jia Hai-Long Pang Han-Qing Feng Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves Bioresources and Bioprocessing Plant growth regulators Agrobacterium-mediated transformation Geminivirus-derived vector Transient expression |
| title | Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves |
| title_full | Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves |
| title_fullStr | Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves |
| title_full_unstemmed | Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves |
| title_short | Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves |
| title_sort | effects of plant growth regulators on transient expression of foreign gene in nicotiana benthamiana l leaves |
| topic | Plant growth regulators Agrobacterium-mediated transformation Geminivirus-derived vector Transient expression |
| url | https://doi.org/10.1186/s40643-021-00480-5 |
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