Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve
Schwann cells (SCs) are constituents of the peripheral nervous system. The differentiation of SCs in injured peripheral nerves is critical for regeneration after injury. Methylcobalamin (MeCbl) is a vitamin B12 analog that is necessary for the maintenance of the peripheral nervous system. In this st...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-08-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00298/full |
| _version_ | 1818858081152401408 |
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| author | Shunsuke eNishimoto Hiroyuki eTanaka Michio eOkamoto Kiyoshi eOkada Kiyoshi eOkada Tsuyoshi eMurase Hideki eYoshikawa |
| author_facet | Shunsuke eNishimoto Hiroyuki eTanaka Michio eOkamoto Kiyoshi eOkada Kiyoshi eOkada Tsuyoshi eMurase Hideki eYoshikawa |
| author_sort | Shunsuke eNishimoto |
| collection | DOAJ |
| description | Schwann cells (SCs) are constituents of the peripheral nervous system. The differentiation of SCs in injured peripheral nerves is critical for regeneration after injury. Methylcobalamin (MeCbl) is a vitamin B12 analog that is necessary for the maintenance of the peripheral nervous system. In this study, we estimated the effect of MeCbl on SCs. We showed that MeCbl downregulated the activity of Erk1/2 and promoted the expression of the myelin basic protein in SCs. In a dorsal root ganglion neuron–SC coculture system, myelination was promoted by MeCbl. In a focal demyelination rat model, MeCbl promoted remyelination and motor and sensory functional regeneration. MeCbl promoted the in vitro differentiation of SCs and in vivo myelination in a rat demyelination model and may be a novel therapy for several types of nervous disorders. |
| first_indexed | 2024-12-19T08:50:37Z |
| format | Article |
| id | doaj.art-081e1eb173164a72b4e975f880f435f4 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-19T08:50:37Z |
| publishDate | 2015-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-081e1eb173164a72b4e975f880f435f42022-12-21T20:28:44ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022015-08-01910.3389/fncel.2015.00298150637Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerveShunsuke eNishimoto0Hiroyuki eTanaka1Michio eOkamoto2Kiyoshi eOkada3Kiyoshi eOkada4Tsuyoshi eMurase5Hideki eYoshikawa6Osaka University Graduate School of MedicineOsaka University Graduate School of MedicineToyonaka Municipal HospitalOsaka University Graduate School of MedicineOsaka University HospitalOsaka University Graduate School of MedicineOsaka University Graduate School of MedicineSchwann cells (SCs) are constituents of the peripheral nervous system. The differentiation of SCs in injured peripheral nerves is critical for regeneration after injury. Methylcobalamin (MeCbl) is a vitamin B12 analog that is necessary for the maintenance of the peripheral nervous system. In this study, we estimated the effect of MeCbl on SCs. We showed that MeCbl downregulated the activity of Erk1/2 and promoted the expression of the myelin basic protein in SCs. In a dorsal root ganglion neuron–SC coculture system, myelination was promoted by MeCbl. In a focal demyelination rat model, MeCbl promoted remyelination and motor and sensory functional regeneration. MeCbl promoted the in vitro differentiation of SCs and in vivo myelination in a rat demyelination model and may be a novel therapy for several types of nervous disorders.http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00298/fullPeripheral Nervous SystemcAMPMyelinationERK signalingMyelin Basic Proteindemyelinating disease |
| spellingShingle | Shunsuke eNishimoto Hiroyuki eTanaka Michio eOkamoto Kiyoshi eOkada Kiyoshi eOkada Tsuyoshi eMurase Hideki eYoshikawa Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve Frontiers in Cellular Neuroscience Peripheral Nervous System cAMP Myelination ERK signaling Myelin Basic Protein demyelinating disease |
| title | Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve |
| title_full | Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve |
| title_fullStr | Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve |
| title_full_unstemmed | Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve |
| title_short | Methylcobalamin promotes the differentiation of Schwann cells and remyelination in lysophosphatidylcholine-induced demyelination of the rat sciatic nerve |
| title_sort | methylcobalamin promotes the differentiation of schwann cells and remyelination in lysophosphatidylcholine induced demyelination of the rat sciatic nerve |
| topic | Peripheral Nervous System cAMP Myelination ERK signaling Myelin Basic Protein demyelinating disease |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00298/full |
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