Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells
Abstract Introduction Ubiquitously found in all life forms, inorganic polyphosphates (polyP) are linear polymers of repeated orthophosphate units. Present in intervertebral disc tissue, polyP was previously shown to increase extracellular matrix production in nucleus pulposus (NP) cells. However, th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-06-01
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| Series: | JOR Spine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jsp2.1143 |
| _version_ | 1818618358122151936 |
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| author | Xiangjiang Wang Rahul Gawri Changbin Lei Joon Lee Gwendolyn Sowa Rita Kandel Nam Vo |
| author_facet | Xiangjiang Wang Rahul Gawri Changbin Lei Joon Lee Gwendolyn Sowa Rita Kandel Nam Vo |
| author_sort | Xiangjiang Wang |
| collection | DOAJ |
| description | Abstract Introduction Ubiquitously found in all life forms, inorganic polyphosphates (polyP) are linear polymers of repeated orthophosphate units. Present in intervertebral disc tissue, polyP was previously shown to increase extracellular matrix production in nucleus pulposus (NP) cells. However, the effects of polyP on human annulus fibrosus (hAF) cell metabolism is not known. Methods and Results Here, hAF cells cultured in the presence of 0.5 to 1 mM polyP, chain length 22 (polyP‐22), showed an increase in glycosaminoglycan content, proteoglycan and collagen synthesis, and aggrecan and collagen type 1 gene expression. Gene expression level of matrix metalloproteinases 1 was reduced while matrix metalloproteinases 3 level was increased in hAF cells treated with 1 mM polyP. Adenosine triphosphate (ATP) synthesis was also significantly increased in hAF cell culture 72 hours after the exposure to 1 mM polyP‐22. Conclusions PolyP thus has both anabolic and bioenergetic effects in AF cells, similar to that observed in NP cells. Together, these results suggest polyP as a potential energy source and a metabolic regulator of disc cells. |
| first_indexed | 2024-12-16T17:20:19Z |
| format | Article |
| id | doaj.art-081ec7a9208640fab6dc39348b0abf1f |
| institution | Directory Open Access Journal |
| issn | 2572-1143 |
| language | English |
| last_indexed | 2024-12-16T17:20:19Z |
| publishDate | 2021-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | JOR Spine |
| spelling | doaj.art-081ec7a9208640fab6dc39348b0abf1f2022-12-21T22:23:10ZengWileyJOR Spine2572-11432021-06-0142n/an/a10.1002/jsp2.1143Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cellsXiangjiang Wang0Rahul Gawri1Changbin Lei2Joon Lee3Gwendolyn Sowa4Rita Kandel5Nam Vo6McGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh Pennsylvania USALunenfeld‐Tanenbaum Research Institute Mount Sinai Hospital Toronto CanadaDepartment of Orthopaedic Surgery University of Pittsburgh Pittsburgh Pennsylvania USAMcGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh Pennsylvania USADepartment of Orthopaedic Surgery University of Pittsburgh Pittsburgh Pennsylvania USALunenfeld‐Tanenbaum Research Institute Mount Sinai Hospital Toronto CanadaMcGowan Institute for Regenerative Medicine University of Pittsburgh Pittsburgh Pennsylvania USAAbstract Introduction Ubiquitously found in all life forms, inorganic polyphosphates (polyP) are linear polymers of repeated orthophosphate units. Present in intervertebral disc tissue, polyP was previously shown to increase extracellular matrix production in nucleus pulposus (NP) cells. However, the effects of polyP on human annulus fibrosus (hAF) cell metabolism is not known. Methods and Results Here, hAF cells cultured in the presence of 0.5 to 1 mM polyP, chain length 22 (polyP‐22), showed an increase in glycosaminoglycan content, proteoglycan and collagen synthesis, and aggrecan and collagen type 1 gene expression. Gene expression level of matrix metalloproteinases 1 was reduced while matrix metalloproteinases 3 level was increased in hAF cells treated with 1 mM polyP. Adenosine triphosphate (ATP) synthesis was also significantly increased in hAF cell culture 72 hours after the exposure to 1 mM polyP‐22. Conclusions PolyP thus has both anabolic and bioenergetic effects in AF cells, similar to that observed in NP cells. Together, these results suggest polyP as a potential energy source and a metabolic regulator of disc cells.https://doi.org/10.1002/jsp2.1143annulus fibrosusdisc degenerationinorganic polyphosphateintervertebral discproteoglycan matrix |
| spellingShingle | Xiangjiang Wang Rahul Gawri Changbin Lei Joon Lee Gwendolyn Sowa Rita Kandel Nam Vo Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells JOR Spine annulus fibrosus disc degeneration inorganic polyphosphate intervertebral disc proteoglycan matrix |
| title | Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells |
| title_full | Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells |
| title_fullStr | Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells |
| title_full_unstemmed | Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells |
| title_short | Inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells |
| title_sort | inorganic polyphosphates stimulates matrix production in human annulus fibrosus cells |
| topic | annulus fibrosus disc degeneration inorganic polyphosphate intervertebral disc proteoglycan matrix |
| url | https://doi.org/10.1002/jsp2.1143 |
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