Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada)
The periodical cicadas of the genus Magicicada Davis, 1925 inhabiting eastern United States are characterized by a long juvenile period of 17 or 13 years and periodical mass emergence of adults. In this genus, only seven species are distinguished and are distributed into three species groups, Decim,...
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Institute of Entomology, Biology Centre, Czech Academy of Science
2020-12-01
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| Series: | European Journal of Entomology |
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| Online Access: | https://www.eje.cz/artkey/eje-202001-0050_cytogenetic_characterization_of_periodical_cicadas_hemiptera_cicadidae_magicicada.php |
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| author | Gayane KARAGYAN Natalia GOLUB Teiji SOTA |
| author_facet | Gayane KARAGYAN Natalia GOLUB Teiji SOTA |
| author_sort | Gayane KARAGYAN |
| collection | DOAJ |
| description | The periodical cicadas of the genus Magicicada Davis, 1925 inhabiting eastern United States are characterized by a long juvenile period of 17 or 13 years and periodical mass emergence of adults. In this genus, only seven species are distinguished and are distributed into three species groups, Decim, Cassini and Decula. We performed comparative cytogenetic study of all seven Magicicada species, and 6 of them were explored for the first time. The techniques used included standard chromosome staining, differential chromosome staining techniques (C-banding, AgNOR-banding and fluorochrome DAPI-staining) and FISH (fluorescence in situ hybridization) with 18S rDNA and (TTAGG)n-telomeric probes. All these species have holokinetic chromosomes, like other Hemiptera. Karyotypes of all species appeared to be remarkably conserved with 2n = 19/20 (18 + X0/XX) (male/female); one very large pair of autosomes and all other chromosomes constituting a decreasing size series; X chromosomes were indistinguishable, at least, at mitotic metaphases. The nucleolus organizer regions (NORs) revealed on one of the medium-sized chromosome pairs in all species and were shown to coincide with the rDNA clusters revealed by FISH. In all species, very small blocks of C-heterochromatin were variously located on chromosomes clearly using DAPI but less distinctly by C-banding. The chromosomes of all the species studied were confirmed to contain the "insect type" motif of telomeres (TTAGG)n. |
| first_indexed | 2024-12-23T04:34:16Z |
| format | Article |
| id | doaj.art-0822d924d102476aaca8201434c965cd |
| institution | Directory Open Access Journal |
| issn | 1210-5759 1802-8829 |
| language | English |
| last_indexed | 2024-12-23T04:34:16Z |
| publishDate | 2020-12-01 |
| publisher | Institute of Entomology, Biology Centre, Czech Academy of Science |
| record_format | Article |
| series | European Journal of Entomology |
| spelling | doaj.art-0822d924d102476aaca8201434c965cd2022-12-21T17:59:56ZengInstitute of Entomology, Biology Centre, Czech Academy of ScienceEuropean Journal of Entomology1210-57591802-88292020-12-01117147448010.14411/eje.2020.050eje-202001-0050Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada)Gayane KARAGYAN0Natalia GOLUB1Teiji SOTA2Scientific Center of Zoology and Hydroecology, NAS RA, P. Sevak 7, Yerevan, 0014 Armenia; e-mail: gaykaragyan@yahoo.comZoological Institute, Russian Academy of Sciences, Universitetskaya nab. 1, 199034 St. Petersburg, Russia; e-mail: nvgolub@mail.ruDepartment of Zoology, Graduate School of Science, Kyoto University, Sakyo, Kyoto, 606-8502 Japan; e-mail: sota@terra.zool.kyoto-u.ac.jpThe periodical cicadas of the genus Magicicada Davis, 1925 inhabiting eastern United States are characterized by a long juvenile period of 17 or 13 years and periodical mass emergence of adults. In this genus, only seven species are distinguished and are distributed into three species groups, Decim, Cassini and Decula. We performed comparative cytogenetic study of all seven Magicicada species, and 6 of them were explored for the first time. The techniques used included standard chromosome staining, differential chromosome staining techniques (C-banding, AgNOR-banding and fluorochrome DAPI-staining) and FISH (fluorescence in situ hybridization) with 18S rDNA and (TTAGG)n-telomeric probes. All these species have holokinetic chromosomes, like other Hemiptera. Karyotypes of all species appeared to be remarkably conserved with 2n = 19/20 (18 + X0/XX) (male/female); one very large pair of autosomes and all other chromosomes constituting a decreasing size series; X chromosomes were indistinguishable, at least, at mitotic metaphases. The nucleolus organizer regions (NORs) revealed on one of the medium-sized chromosome pairs in all species and were shown to coincide with the rDNA clusters revealed by FISH. In all species, very small blocks of C-heterochromatin were variously located on chromosomes clearly using DAPI but less distinctly by C-banding. The chromosomes of all the species studied were confirmed to contain the "insect type" motif of telomeres (TTAGG)n.https://www.eje.cz/artkey/eje-202001-0050_cytogenetic_characterization_of_periodical_cicadas_hemiptera_cicadidae_magicicada.phphemipteracicadidaemagicicadaperiodical cicadaschromosome numberskaryotypec-heterochromatinnucleolus organizer regionsat-rich regions18s rdnatelomeres |
| spellingShingle | Gayane KARAGYAN Natalia GOLUB Teiji SOTA Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada) European Journal of Entomology hemiptera cicadidae magicicada periodical cicadas chromosome numbers karyotype c-heterochromatin nucleolus organizer regions at-rich regions 18s rdna telomeres |
| title | Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada) |
| title_full | Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada) |
| title_fullStr | Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada) |
| title_full_unstemmed | Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada) |
| title_short | Cytogenetic characterization of periodical cicadas (Hemiptera: Cicadidae: Magicicada) |
| title_sort | cytogenetic characterization of periodical cicadas hemiptera cicadidae magicicada |
| topic | hemiptera cicadidae magicicada periodical cicadas chromosome numbers karyotype c-heterochromatin nucleolus organizer regions at-rich regions 18s rdna telomeres |
| url | https://www.eje.cz/artkey/eje-202001-0050_cytogenetic_characterization_of_periodical_cicadas_hemiptera_cicadidae_magicicada.php |
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