Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging
Cell is the structural and functional unit of organism. It serves as a key research object in various biological processes, such as growth, ontogeny, metabolism and stress. Studying the spatiotemporal distribution and functional activity of specific biological molecules in living cells is crucial fo...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-12-01
|
| Series: | Frontiers in Chemistry |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2020.598013/full |
| _version_ | 1818664321748566016 |
|---|---|
| author | Yutong Zhang Yulin Du Yuting Zhuo Yuting Zhuo Liping Qiu Liping Qiu |
| author_facet | Yutong Zhang Yulin Du Yuting Zhuo Yuting Zhuo Liping Qiu Liping Qiu |
| author_sort | Yutong Zhang |
| collection | DOAJ |
| description | Cell is the structural and functional unit of organism. It serves as a key research object in various biological processes, such as growth, ontogeny, metabolism and stress. Studying the spatiotemporal distribution and functional activity of specific biological molecules in living cells is crucial for exploring the mechanism governing life. It also facilitates the elucidation of pathogenesis, clinical prevention and disease theranostics. In recent years, the fluorescence imaging technique has been greatly exploited for live-cell imaging. However, the development of molecular probes has lagged far behind. Functional nucleic acids (FNAs), for example, aptamer and DNAzyme, possess special chemical and/or biological functions, hence severing as promising molecular tools for cellular imaging. The current mini review focuses on the applications of FNAs in live-cell fluorescence imaging. |
| first_indexed | 2024-12-17T05:30:53Z |
| format | Article |
| id | doaj.art-0826311197864133a3d4cd653aa3f11f |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-17T05:30:53Z |
| publishDate | 2020-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-0826311197864133a3d4cd653aa3f11f2022-12-21T22:01:44ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462020-12-01810.3389/fchem.2020.598013598013Functional Nucleic Acid-Based Live-Cell Fluorescence ImagingYutong Zhang0Yulin Du1Yuting Zhuo2Yuting Zhuo3Liping Qiu4Liping Qiu5Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, ChinaMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, ChinaMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, ChinaNHC Key Laboratory of Birth Defect for Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, ChinaMolecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, ChinaNHC Key Laboratory of Birth Defect for Research and Prevention, Hunan Provincial Maternal and Child Health Care Hospital, Changsha, ChinaCell is the structural and functional unit of organism. It serves as a key research object in various biological processes, such as growth, ontogeny, metabolism and stress. Studying the spatiotemporal distribution and functional activity of specific biological molecules in living cells is crucial for exploring the mechanism governing life. It also facilitates the elucidation of pathogenesis, clinical prevention and disease theranostics. In recent years, the fluorescence imaging technique has been greatly exploited for live-cell imaging. However, the development of molecular probes has lagged far behind. Functional nucleic acids (FNAs), for example, aptamer and DNAzyme, possess special chemical and/or biological functions, hence severing as promising molecular tools for cellular imaging. The current mini review focuses on the applications of FNAs in live-cell fluorescence imaging.https://www.frontiersin.org/articles/10.3389/fchem.2020.598013/fulllive-cell imagingaptamerDNAzymefluorescence probefunctional nucleic acid |
| spellingShingle | Yutong Zhang Yulin Du Yuting Zhuo Yuting Zhuo Liping Qiu Liping Qiu Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging Frontiers in Chemistry live-cell imaging aptamer DNAzyme fluorescence probe functional nucleic acid |
| title | Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging |
| title_full | Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging |
| title_fullStr | Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging |
| title_full_unstemmed | Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging |
| title_short | Functional Nucleic Acid-Based Live-Cell Fluorescence Imaging |
| title_sort | functional nucleic acid based live cell fluorescence imaging |
| topic | live-cell imaging aptamer DNAzyme fluorescence probe functional nucleic acid |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2020.598013/full |
| work_keys_str_mv | AT yutongzhang functionalnucleicacidbasedlivecellfluorescenceimaging AT yulindu functionalnucleicacidbasedlivecellfluorescenceimaging AT yutingzhuo functionalnucleicacidbasedlivecellfluorescenceimaging AT yutingzhuo functionalnucleicacidbasedlivecellfluorescenceimaging AT lipingqiu functionalnucleicacidbasedlivecellfluorescenceimaging AT lipingqiu functionalnucleicacidbasedlivecellfluorescenceimaging |