In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy
Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacousti...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/21/5/1776 |
| _version_ | 1797414496596131840 |
|---|---|
| author | Thi Thao Mai Su Woong Yoo Suhyun Park Jin Young Kim Kang-Ho Choi Chulhong Kim Seong Young Kwon Jung-Joon Min Changho Lee |
| author_facet | Thi Thao Mai Su Woong Yoo Suhyun Park Jin Young Kim Kang-Ho Choi Chulhong Kim Seong Young Kwon Jung-Joon Min Changho Lee |
| author_sort | Thi Thao Mai |
| collection | DOAJ |
| description | Vascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method. |
| first_indexed | 2024-03-09T05:34:07Z |
| format | Article |
| id | doaj.art-08280ab74abb457392cb5224a6171ba7 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T05:34:07Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-08280ab74abb457392cb5224a6171ba72023-12-03T12:29:37ZengMDPI AGSensors1424-82202021-03-01215177610.3390/s21051776In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic MicroscopyThi Thao Mai0Su Woong Yoo1Suhyun Park2Jin Young Kim3Kang-Ho Choi4Chulhong Kim5Seong Young Kwon6Jung-Joon Min7Changho Lee8Department of Artificial Intelligence Convergence, Chonnam National University, Gwangju 61186, KoreaDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 58128, KoreaInterdisciplinary Program of Molecular Medicine, Chonnam National University, Gwangju 61186, KoreaDepartment of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk-do 37673, KoreaDepartment of Neurology, Chonnam National University Hospital, 8 Hak-dong, Dong-gu, Gwangju 501-757, KoreaDepartment of Creative IT Engineering and Electrical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, Gyeongbuk-do 37673, KoreaDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 58128, KoreaDepartment of Nuclear Medicine, Chonnam National University Hwasun Hospital, Hwasun, Jeollanamdo 58128, KoreaDepartment of Artificial Intelligence Convergence, Chonnam National University, Gwangju 61186, KoreaVascular damage is one of the therapeutic mechanisms of photodynamic therapy (PDT). In particular, short-term PDT treatments can effectively destroy malignant lesions while minimizing damage to nonmalignant tissue. In this study, we investigate the feasibility of label-free quantitative photoacoustic microscopy (PAM) for monitoring the vasculature changes under the effect of PDT in mouse ear melanoma tumors. In particular, quantitative vasculature evaluation was conducted based on Hessian filter segmentation. Three-dimensional morphological PAM and depth-resolved images before and after PDT treatment were acquired. In addition, five quantitative vasculature parameters, including the PA signal, vessel diameter, vessel density, perfused vessel density, and vessel complexity, were analyzed to evaluate the influence of PDT on four different areas: Two melanoma tumors, and control and normal vessel areas. The quantitative and qualitative results successfully demonstrated the potential of the proposed PAM-based quantitative approach to evaluate the effectiveness of the PDT method.https://www.mdpi.com/1424-8220/21/5/1776photodynamic therapyphotoacoustic microscopyquantitative analysis |
| spellingShingle | Thi Thao Mai Su Woong Yoo Suhyun Park Jin Young Kim Kang-Ho Choi Chulhong Kim Seong Young Kwon Jung-Joon Min Changho Lee In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy Sensors photodynamic therapy photoacoustic microscopy quantitative analysis |
| title | In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy |
| title_full | In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy |
| title_fullStr | In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy |
| title_full_unstemmed | In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy |
| title_short | In Vivo Quantitative Vasculature Segmentation and Assessment for Photodynamic Therapy Process Monitoring Using Photoacoustic Microscopy |
| title_sort | in vivo quantitative vasculature segmentation and assessment for photodynamic therapy process monitoring using photoacoustic microscopy |
| topic | photodynamic therapy photoacoustic microscopy quantitative analysis |
| url | https://www.mdpi.com/1424-8220/21/5/1776 |
| work_keys_str_mv | AT thithaomai invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT suwoongyoo invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT suhyunpark invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT jinyoungkim invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT kanghochoi invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT chulhongkim invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT seongyoungkwon invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT jungjoonmin invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy AT changholee invivoquantitativevasculaturesegmentationandassessmentforphotodynamictherapyprocessmonitoringusingphotoacousticmicroscopy |