Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood
Extracellular vesicles (EVs) have great potential as biomarkers since their composition and concentration in biofluids are disease state dependent and their cargo can contain disease-related information. Large tumor-derived EVs (tdEVs, >1 μm) in blood from cancer patients are associated with...
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.00608/full |
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| author | L. G. Rikkert L. G. Rikkert L. G. Rikkert P. Beekman P. Beekman P. Beekman J. Caro F. A. W. Coumans F. A. W. Coumans A. Enciso-Martinez G. Jenster S. Le Gac W. Lee T. G. van Leeuwen T. G. van Leeuwen G. B. Loozen A. Nanou R. Nieuwland R. Nieuwland H. L. Offerhaus C. Otto D. M. Pegtel M. C. Piontek E. van der Pol E. van der Pol E. van der Pol L. de Rond L. de Rond L. de Rond W. H. Roos R. B. M. Schasfoort M. H. M. Wauben H. Zuilhof H. Zuilhof L. W. M. M. Terstappen |
| author_facet | L. G. Rikkert L. G. Rikkert L. G. Rikkert P. Beekman P. Beekman P. Beekman J. Caro F. A. W. Coumans F. A. W. Coumans A. Enciso-Martinez G. Jenster S. Le Gac W. Lee T. G. van Leeuwen T. G. van Leeuwen G. B. Loozen A. Nanou R. Nieuwland R. Nieuwland H. L. Offerhaus C. Otto D. M. Pegtel M. C. Piontek E. van der Pol E. van der Pol E. van der Pol L. de Rond L. de Rond L. de Rond W. H. Roos R. B. M. Schasfoort M. H. M. Wauben H. Zuilhof H. Zuilhof L. W. M. M. Terstappen |
| author_sort | L. G. Rikkert |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) have great potential as biomarkers since their composition and concentration in biofluids are disease state dependent and their cargo can contain disease-related information. Large tumor-derived EVs (tdEVs, >1 μm) in blood from cancer patients are associated with poor outcome, and changes in their number can be used to monitor therapy effectiveness. Whereas, small tumor-derived EVs (<1 μm) are likely to outnumber their larger counterparts, thereby offering better statistical significance, identification and quantification of small tdEVs are more challenging. In the blood of cancer patients, a subpopulation of EVs originate from tumor cells, but these EVs are outnumbered by non-EV particles and EVs from other origin. In the Dutch NWO Perspectief Cancer-ID program, we developed and evaluated detection and characterization techniques to distinguish EVs from non-EV particles and other EVs. Despite low signal amplitudes, we identified characteristics of these small tdEVs that may enable the enumeration of small tdEVs and extract relevant information. The insights obtained from Cancer-ID can help to explore the full potential of tdEVs in the clinic. |
| first_indexed | 2024-12-17T03:17:00Z |
| format | Article |
| id | doaj.art-082acfe9a39b48b295204f818f431623 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-17T03:17:00Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-082acfe9a39b48b295204f818f4316232022-12-21T22:05:38ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-06-011010.3389/fonc.2020.00608527735Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in BloodL. G. Rikkert0L. G. Rikkert1L. G. Rikkert2P. Beekman3P. Beekman4P. Beekman5J. Caro6F. A. W. Coumans7F. A. W. Coumans8A. Enciso-Martinez9G. Jenster10S. Le Gac11W. Lee12T. G. van Leeuwen13T. G. van Leeuwen14G. B. Loozen15A. Nanou16R. Nieuwland17R. Nieuwland18H. L. Offerhaus19C. Otto20D. M. Pegtel21M. C. Piontek22E. van der Pol23E. van der Pol24E. van der Pol25L. de Rond26L. de Rond27L. de Rond28W. H. Roos29R. B. M. Schasfoort30M. H. M. Wauben31H. Zuilhof32H. Zuilhof33L. W. M. M. Terstappen34Department of Medical Cell Biophysics, University of Twente, Enschede, NetherlandsLaboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsVesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsDepartment of Medical Cell Biophysics, University of Twente, Enschede, NetherlandsLaboratory of Organic Chemistry, Wageningen University, Wageningen, NetherlandsApplied Microfluidics for Bioengineering Research, University of Twente, Enschede, NetherlandsDepartment of Imaging Physics, Delft University of Technology, Delft, NetherlandsLaboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsVesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsDepartment of Medical Cell Biophysics, University of Twente, Enschede, NetherlandsDepartment of Urology, Erasmus University Medical Center, Rotterdam, NetherlandsApplied Microfluidics for Bioengineering Research, University of Twente, Enschede, NetherlandsOptical Sciences Group, Department of Science and Technology, University of Twente, Enschede, NetherlandsVesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsBiomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsDepartment of Imaging Physics, Delft University of Technology, Delft, NetherlandsDepartment of Medical Cell Biophysics, University of Twente, Enschede, NetherlandsLaboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsVesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsOptical Sciences Group, Department of Science and Technology, University of Twente, Enschede, NetherlandsDepartment of Medical Cell Biophysics, University of Twente, Enschede, Netherlands0Department of Pathology, Amsterdam UMC, VU University Amsterdam, Amsterdam, Netherlands1Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, NetherlandsLaboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsVesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsBiomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsLaboratory of Experimental Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsVesicle Observation Center, Amsterdam UMC, University of Amsterdam, Amsterdam, NetherlandsBiomedical Engineering and Physics, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands1Molecular Biophysics, Zernike Institute, University of Groningen, Groningen, NetherlandsDepartment of Medical Cell Biophysics, University of Twente, Enschede, Netherlands2Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NetherlandsLaboratory of Organic Chemistry, Wageningen University, Wageningen, Netherlands3School of Pharmaceutical Sciences and Technology, Tianjin University, Tianjin, ChinaDepartment of Medical Cell Biophysics, University of Twente, Enschede, NetherlandsExtracellular vesicles (EVs) have great potential as biomarkers since their composition and concentration in biofluids are disease state dependent and their cargo can contain disease-related information. Large tumor-derived EVs (tdEVs, >1 μm) in blood from cancer patients are associated with poor outcome, and changes in their number can be used to monitor therapy effectiveness. Whereas, small tumor-derived EVs (<1 μm) are likely to outnumber their larger counterparts, thereby offering better statistical significance, identification and quantification of small tdEVs are more challenging. In the blood of cancer patients, a subpopulation of EVs originate from tumor cells, but these EVs are outnumbered by non-EV particles and EVs from other origin. In the Dutch NWO Perspectief Cancer-ID program, we developed and evaluated detection and characterization techniques to distinguish EVs from non-EV particles and other EVs. Despite low signal amplitudes, we identified characteristics of these small tdEVs that may enable the enumeration of small tdEVs and extract relevant information. The insights obtained from Cancer-ID can help to explore the full potential of tdEVs in the clinic.https://www.frontiersin.org/article/10.3389/fonc.2020.00608/fullatomic force microscopyelectrochemistryelectron microscopyextracellular vesiclesflow cytometryfluorescence microscopy |
| spellingShingle | L. G. Rikkert L. G. Rikkert L. G. Rikkert P. Beekman P. Beekman P. Beekman J. Caro F. A. W. Coumans F. A. W. Coumans A. Enciso-Martinez G. Jenster S. Le Gac W. Lee T. G. van Leeuwen T. G. van Leeuwen G. B. Loozen A. Nanou R. Nieuwland R. Nieuwland H. L. Offerhaus C. Otto D. M. Pegtel M. C. Piontek E. van der Pol E. van der Pol E. van der Pol L. de Rond L. de Rond L. de Rond W. H. Roos R. B. M. Schasfoort M. H. M. Wauben H. Zuilhof H. Zuilhof L. W. M. M. Terstappen Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood Frontiers in Oncology atomic force microscopy electrochemistry electron microscopy extracellular vesicles flow cytometry fluorescence microscopy |
| title | Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood |
| title_full | Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood |
| title_fullStr | Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood |
| title_full_unstemmed | Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood |
| title_short | Cancer-ID: Toward Identification of Cancer by Tumor-Derived Extracellular Vesicles in Blood |
| title_sort | cancer id toward identification of cancer by tumor derived extracellular vesicles in blood |
| topic | atomic force microscopy electrochemistry electron microscopy extracellular vesicles flow cytometry fluorescence microscopy |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.00608/full |
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