Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes
The present study aimed to investigate the genetic variation and genetic structure of the Phenacoccus manihoti Matile-Ferrero, one of the most serious insect pests of cassava worldwide, in populations in Thailand, using mitochondrial and nuclear DNA sequence based analysis. The samples of P. manihot...
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| Format: | Article |
| Language: | English |
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Walailak University
2015-01-01
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| Series: | Walailak Journal of Science and Technology |
| Subjects: | |
| Online Access: | http://wjst.wu.ac.th/index.php/wjst/article/view/1432 |
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| author | Atsalek RATTANAWANNEE Wiboon CHONGRATTANAMETEEKUL |
| author_facet | Atsalek RATTANAWANNEE Wiboon CHONGRATTANAMETEEKUL |
| author_sort | Atsalek RATTANAWANNEE |
| collection | DOAJ |
| description | The present study aimed to investigate the genetic variation and genetic structure of the Phenacoccus manihoti Matile-Ferrero, one of the most serious insect pests of cassava worldwide, in populations in Thailand, using mitochondrial and nuclear DNA sequence based analysis. The samples of P. manihoti were collected from 28 major cassava-growing areas within 18 provinces in Thailand. Our field survey results showed that the northeastern and eastern regions of Thailand were widely and highly infested with P. manihoti. Phylogenetic analysis revealed 2 mitochondrial clades and a single nuclear clade, which corresponded to low genetic variability. This suggests that P. manihoti has a high potential to spread aggressively throughout the cassava-growing areas in Thailand that in which it was first found in 2008. In addition, the generally low genetic divergence observed may be due to the highly prevalent parthenogenetic reproduction of this insect pest species. Further research is therefore necessary to develop proportional prevention and surveillance programs for early detection and rapid response. In addition, the genetic structure and variability of P. manihoti populations from neighboring countries should be studied.
doi:10.14456/WJST.2016.13 |
| first_indexed | 2024-12-11T19:29:20Z |
| format | Article |
| id | doaj.art-082cf9ba275b4bfdadca5c61ae9358e7 |
| institution | Directory Open Access Journal |
| issn | 1686-3933 2228-835X |
| language | English |
| last_indexed | 2024-12-11T19:29:20Z |
| publishDate | 2015-01-01 |
| publisher | Walailak University |
| record_format | Article |
| series | Walailak Journal of Science and Technology |
| spelling | doaj.art-082cf9ba275b4bfdadca5c61ae9358e72022-12-22T00:53:19ZengWalailak UniversityWalailak Journal of Science and Technology1686-39332228-835X2015-01-0113210.14456/vol13iss2ppGenetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear GenesAtsalek RATTANAWANNEE0Wiboon CHONGRATTANAMETEEKUL1Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900The present study aimed to investigate the genetic variation and genetic structure of the Phenacoccus manihoti Matile-Ferrero, one of the most serious insect pests of cassava worldwide, in populations in Thailand, using mitochondrial and nuclear DNA sequence based analysis. The samples of P. manihoti were collected from 28 major cassava-growing areas within 18 provinces in Thailand. Our field survey results showed that the northeastern and eastern regions of Thailand were widely and highly infested with P. manihoti. Phylogenetic analysis revealed 2 mitochondrial clades and a single nuclear clade, which corresponded to low genetic variability. This suggests that P. manihoti has a high potential to spread aggressively throughout the cassava-growing areas in Thailand that in which it was first found in 2008. In addition, the generally low genetic divergence observed may be due to the highly prevalent parthenogenetic reproduction of this insect pest species. Further research is therefore necessary to develop proportional prevention and surveillance programs for early detection and rapid response. In addition, the genetic structure and variability of P. manihoti populations from neighboring countries should be studied. doi:10.14456/WJST.2016.13http://wjst.wu.ac.th/index.php/wjst/article/view/1432Genetic variationcassava mealybugPhenacoccus manihotiDNA sequence |
| spellingShingle | Atsalek RATTANAWANNEE Wiboon CHONGRATTANAMETEEKUL Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes Walailak Journal of Science and Technology Genetic variation cassava mealybug Phenacoccus manihoti DNA sequence |
| title | Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes |
| title_full | Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes |
| title_fullStr | Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes |
| title_full_unstemmed | Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes |
| title_short | Genetic Variation of Cassava Mealybug, Phenacoccus manihoti (Hemiptera: Pseudococcidae), Based on DNA Sequences from Mitochondrial and Nuclear Genes |
| title_sort | genetic variation of cassava mealybug phenacoccus manihoti hemiptera pseudococcidae based on dna sequences from mitochondrial and nuclear genes |
| topic | Genetic variation cassava mealybug Phenacoccus manihoti DNA sequence |
| url | http://wjst.wu.ac.th/index.php/wjst/article/view/1432 |
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