Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions
The proliferation of extravillous trophoblasts (EVT) and their further migration, invasion, and differentiation into the decidual and myometrial vasculature are vital for spiral artery remodeling. These physiological functions of EVT are also essential steps in the implantation of the human embryo a...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023001652 |
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| author | Zhenni Mu Sinan Shen Li Tang Yingdie Liu Ziwei Zhou Lei Lei |
| author_facet | Zhenni Mu Sinan Shen Li Tang Yingdie Liu Ziwei Zhou Lei Lei |
| author_sort | Zhenni Mu |
| collection | DOAJ |
| description | The proliferation of extravillous trophoblasts (EVT) and their further migration, invasion, and differentiation into the decidual and myometrial vasculature are vital for spiral artery remodeling. These physiological functions of EVT are also essential steps in the implantation of the human embryo and the formation of the placenta and are closely related to pregnancy maintenance and the occurrence of abortion. Hyperin is a flavonoid with anti-inflammatory, pro-proliferative, and anti-apoptotic properties. Consequently, we investigated the previously unexplored effects of hyperin on the proliferation, migration, and invasion of HTR-8/SVneo cells. Human extravillous trophoblast-derived HTR-8/SVneo cells were incubated with different concentrations of hyperin (0, 5, 10, 25, 50, and 100 μM) to observe the changes in cell proliferation, migration, invasive capacity, and pathway activation. Proliferation, migration, and invasion were promoted by activating the JAK1/STAT3 pathway in HTR-8/SVneo cells treated with hyperin. In addition, brepocitinib (PF-06700841) significantly inhibited the proliferation, migration, and invasion effects of hyperin on HTR-8/SVneo cells. In vivo experiments confirmed that hyperin reduces the embryo loss rate in recurrent spontaneous abortion (RSA) model mice. Furthermore, our study revealed that hyperin promoted the proliferation, migration, and invasion of HTR-8/SVneo cells via activation of the JAK1/STAT3 pathway, further improving pregnancy outcomes in RSA. |
| first_indexed | 2024-04-10T17:44:59Z |
| format | Article |
| id | doaj.art-083305f624824745a7ed1217ece6e5ba |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T17:44:59Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-083305f624824745a7ed1217ece6e5ba2023-02-03T04:59:54ZengElsevierHeliyon2405-84402023-01-0191e12958Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortionsZhenni Mu0Sinan Shen1Li Tang2Yingdie Liu3Ziwei Zhou4Lei Lei5College of Integrated Traditional and Western Medicine, Hunan University of Chinese Medicine, Changsha, ChinaCollege of Integrated Traditional and Western Medicine, Hunan University of Chinese Medicine, Changsha, ChinaCollege of Integrated Traditional and Western Medicine, Hunan University of Chinese Medicine, Changsha, ChinaThe First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, ChinaThe First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, ChinaCollege of Integrated Traditional and Western Medicine, Hunan University of Chinese Medicine, Changsha, China; Corresponding author.The proliferation of extravillous trophoblasts (EVT) and their further migration, invasion, and differentiation into the decidual and myometrial vasculature are vital for spiral artery remodeling. These physiological functions of EVT are also essential steps in the implantation of the human embryo and the formation of the placenta and are closely related to pregnancy maintenance and the occurrence of abortion. Hyperin is a flavonoid with anti-inflammatory, pro-proliferative, and anti-apoptotic properties. Consequently, we investigated the previously unexplored effects of hyperin on the proliferation, migration, and invasion of HTR-8/SVneo cells. Human extravillous trophoblast-derived HTR-8/SVneo cells were incubated with different concentrations of hyperin (0, 5, 10, 25, 50, and 100 μM) to observe the changes in cell proliferation, migration, invasive capacity, and pathway activation. Proliferation, migration, and invasion were promoted by activating the JAK1/STAT3 pathway in HTR-8/SVneo cells treated with hyperin. In addition, brepocitinib (PF-06700841) significantly inhibited the proliferation, migration, and invasion effects of hyperin on HTR-8/SVneo cells. In vivo experiments confirmed that hyperin reduces the embryo loss rate in recurrent spontaneous abortion (RSA) model mice. Furthermore, our study revealed that hyperin promoted the proliferation, migration, and invasion of HTR-8/SVneo cells via activation of the JAK1/STAT3 pathway, further improving pregnancy outcomes in RSA.http://www.sciencedirect.com/science/article/pii/S2405844023001652HyperinProliferationMigrationInvasionJAK1/STAT3Recurrent spontaneous abortions |
| spellingShingle | Zhenni Mu Sinan Shen Li Tang Yingdie Liu Ziwei Zhou Lei Lei Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions Heliyon Hyperin Proliferation Migration Invasion JAK1/STAT3 Recurrent spontaneous abortions |
| title | Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions |
| title_full | Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions |
| title_fullStr | Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions |
| title_full_unstemmed | Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions |
| title_short | Hyperin promotes proliferation, migration, and invasion of HTR-8/SVneo trophoblast cells via activation of JAK1/STAT3 pathway in recurrent spontaneous abortions |
| title_sort | hyperin promotes proliferation migration and invasion of htr 8 svneo trophoblast cells via activation of jak1 stat3 pathway in recurrent spontaneous abortions |
| topic | Hyperin Proliferation Migration Invasion JAK1/STAT3 Recurrent spontaneous abortions |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023001652 |
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