Non-Specific Reactions during Immunomagnetic Separation of Listeria
Problems occurring during the immunomagnetic separation (IMS) of Listeria using immunomagnetic particles Dynabeads® anti-Listeria (Dynal Biotech, Norway) were specified. Characteristics of these particles were compared with anti-Listeria spp. magnetite particles (Quantum Magnetics, USA). Pure cultur...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2005-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/163011 |
| _version_ | 1797424892115681280 |
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| author | Iveta Zachová Libor Červenka Jana Štepánková Marcela Pejchalová Jarmila Vytrasová |
| author_facet | Iveta Zachová Libor Červenka Jana Štepánková Marcela Pejchalová Jarmila Vytrasová |
| author_sort | Iveta Zachová |
| collection | DOAJ |
| description | Problems occurring during the immunomagnetic separation (IMS) of Listeria using immunomagnetic particles Dynabeads® anti-Listeria (Dynal Biotech, Norway) were specified. Characteristics of these particles were compared with anti-Listeria spp. magnetite particles (Quantum Magnetics, USA). Pure cultures of Listeria innocua, Arthrobacter spp., Bacillus subtilis, Citrobacter braakii, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae and Staphylococcus aureus were used to evaluate non-specific reactions during IMS. Gram-positive microorganisms, especially Staphylococcus aureus and Arthrobacter spp., were found to be responsible for non-specific reactions in most cases. The capacity of Dynabeads® anti-Listeria particles was determined to be about 10 % of the initial pure cultures of Listeria spp., after 10 min of incubation. Non-specific reactions during IMS of Listeria were examined on the artificially inoculated food samples in which Gram-positive bacteria showed the highest percentage of capture. Influence of washing in two buffers was also studied. |
| first_indexed | 2024-03-09T08:08:13Z |
| format | Article |
| id | doaj.art-083462ca1037481091a84df53ada527e |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T08:08:13Z |
| publishDate | 2005-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-083462ca1037481091a84df53ada527e2023-12-02T23:42:25ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062005-01-01434397341Non-Specific Reactions during Immunomagnetic Separation of ListeriaIveta Zachová0Libor Červenka1Jana Štepánková2Marcela Pejchalová3Jarmila Vytrasová4Department of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Štrossova 239, CZ-530 03 Pardubice, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, nám. Čs. legií 565, CZ-532 10 Pardubice, Czech RepublicDepartment of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, nám. Čs. legií 565, CZ-532 10 Pardubice, Czech RepublicDepartment of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Štrossova 239, CZ-530 03 Pardubice, Czech RepublicDepartment of Biological and Biochemical Sciences, Faculty of Chemical Technology, University of Pardubice, Štrossova 239, CZ-530 03 Pardubice, Czech RepublicProblems occurring during the immunomagnetic separation (IMS) of Listeria using immunomagnetic particles Dynabeads® anti-Listeria (Dynal Biotech, Norway) were specified. Characteristics of these particles were compared with anti-Listeria spp. magnetite particles (Quantum Magnetics, USA). Pure cultures of Listeria innocua, Arthrobacter spp., Bacillus subtilis, Citrobacter braakii, Escherichia coli, Enterobacter aerogenes, Enterobacter cloacae and Staphylococcus aureus were used to evaluate non-specific reactions during IMS. Gram-positive microorganisms, especially Staphylococcus aureus and Arthrobacter spp., were found to be responsible for non-specific reactions in most cases. The capacity of Dynabeads® anti-Listeria particles was determined to be about 10 % of the initial pure cultures of Listeria spp., after 10 min of incubation. Non-specific reactions during IMS of Listeria were examined on the artificially inoculated food samples in which Gram-positive bacteria showed the highest percentage of capture. Influence of washing in two buffers was also studied.http://hrcak.srce.hr/file/163011immunomagnetic separationimmunomagnetic particlesnon-specific reactionsListeria |
| spellingShingle | Iveta Zachová Libor Červenka Jana Štepánková Marcela Pejchalová Jarmila Vytrasová Non-Specific Reactions during Immunomagnetic Separation of Listeria Food Technology and Biotechnology immunomagnetic separation immunomagnetic particles non-specific reactions Listeria |
| title | Non-Specific Reactions during Immunomagnetic Separation of Listeria |
| title_full | Non-Specific Reactions during Immunomagnetic Separation of Listeria |
| title_fullStr | Non-Specific Reactions during Immunomagnetic Separation of Listeria |
| title_full_unstemmed | Non-Specific Reactions during Immunomagnetic Separation of Listeria |
| title_short | Non-Specific Reactions during Immunomagnetic Separation of Listeria |
| title_sort | non specific reactions during immunomagnetic separation of listeria |
| topic | immunomagnetic separation immunomagnetic particles non-specific reactions Listeria |
| url | http://hrcak.srce.hr/file/163011 |
| work_keys_str_mv | AT ivetazachova nonspecificreactionsduringimmunomagneticseparationoflisteria AT liborcervenka nonspecificreactionsduringimmunomagneticseparationoflisteria AT janastepankova nonspecificreactionsduringimmunomagneticseparationoflisteria AT marcelapejchalova nonspecificreactionsduringimmunomagneticseparationoflisteria AT jarmilavytrasova nonspecificreactionsduringimmunomagneticseparationoflisteria |