| Summary: | Preeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet completely understood and without curative therapy. The histone modifications H3K4me<sup>3</sup> and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of the development of PE, the influence of Gal-2 on histone modification in trophoblasts and in syncytialisation was investigated. Immunohistochemical stains of 13 PE and 13 control placentas were correlated, followed by cell culture experiments. An analysis of H3K4me<sup>3</sup> and H3K9ac was conducted, as well as cell fusion staining with E-cadherin and β-catenin—both after incubation with Gal-2. The expression of H3K4me<sup>3</sup> and H3K9ac correlated significantly with the expression of Gal-2. Furthermore, we detected an increase in H3K4me<sup>3</sup> and H3K9ac after the addition of Gal-2 to BeWo/HVT cells. Moreover, there was increased fusion of HVT cells after incubation with Gal-2. Gal-2 is associated with the histone modifications H3K4me<sup>3</sup> and H3K9ac in trophoblasts. Furthermore, syncytialisation increased after incubation with Gal-2. Therefore, we postulate that Gal-2 stimulates syncytialisation, possibly mediated by H3K4me<sup>3</sup> and H3K9ac. Since Gal-2, as well as H3K4me<sup>3</sup> and H3K9ac, are decreased in PE, the induction of Gal-2 might be a promising therapeutic target.
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