Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia
Preeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet completely understood and without curative therapy. The histone modifications H3K4me<sup>3</sup> and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of t...
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MDPI AG
2022-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/5/707 |
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| author | Laura Hahn Sarah Meister Mareike Mannewitz Susanne Beyer Stefanie Corradini Uwe Hasbargen Sven Mahner Udo Jeschke Thomas Kolben Alexander Burges |
| author_facet | Laura Hahn Sarah Meister Mareike Mannewitz Susanne Beyer Stefanie Corradini Uwe Hasbargen Sven Mahner Udo Jeschke Thomas Kolben Alexander Burges |
| author_sort | Laura Hahn |
| collection | DOAJ |
| description | Preeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet completely understood and without curative therapy. The histone modifications H3K4me<sup>3</sup> and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of the development of PE, the influence of Gal-2 on histone modification in trophoblasts and in syncytialisation was investigated. Immunohistochemical stains of 13 PE and 13 control placentas were correlated, followed by cell culture experiments. An analysis of H3K4me<sup>3</sup> and H3K9ac was conducted, as well as cell fusion staining with E-cadherin and β-catenin—both after incubation with Gal-2. The expression of H3K4me<sup>3</sup> and H3K9ac correlated significantly with the expression of Gal-2. Furthermore, we detected an increase in H3K4me<sup>3</sup> and H3K9ac after the addition of Gal-2 to BeWo/HVT cells. Moreover, there was increased fusion of HVT cells after incubation with Gal-2. Gal-2 is associated with the histone modifications H3K4me<sup>3</sup> and H3K9ac in trophoblasts. Furthermore, syncytialisation increased after incubation with Gal-2. Therefore, we postulate that Gal-2 stimulates syncytialisation, possibly mediated by H3K4me<sup>3</sup> and H3K9ac. Since Gal-2, as well as H3K4me<sup>3</sup> and H3K9ac, are decreased in PE, the induction of Gal-2 might be a promising therapeutic target. |
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| last_indexed | 2024-03-10T03:15:11Z |
| publishDate | 2022-05-01 |
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| series | Biomolecules |
| spelling | doaj.art-0835b14cf78240cd8d43b8b82f70132f2023-11-23T10:14:24ZengMDPI AGBiomolecules2218-273X2022-05-0112570710.3390/biom12050707Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and PreeclampsiaLaura Hahn0Sarah Meister1Mareike Mannewitz2Susanne Beyer3Stefanie Corradini4Uwe Hasbargen5Sven Mahner6Udo Jeschke7Thomas Kolben8Alexander Burges9Department of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Radiation Oncology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyDepartment of Obsterics and Gynecology, University Hospital, Ludwig-Maximilians-Universität Munich, Marchioninistr. 15, 81337 Munich, GermanyPreeclampsia (PE) is a severe pregnancy disorder with a pathophysiology not yet completely understood and without curative therapy. The histone modifications H3K4me<sup>3</sup> and H3K9ac, as well as galectin-2 (Gal-2), are known to be decreased in PE. To gain a better understanding of the development of PE, the influence of Gal-2 on histone modification in trophoblasts and in syncytialisation was investigated. Immunohistochemical stains of 13 PE and 13 control placentas were correlated, followed by cell culture experiments. An analysis of H3K4me<sup>3</sup> and H3K9ac was conducted, as well as cell fusion staining with E-cadherin and β-catenin—both after incubation with Gal-2. The expression of H3K4me<sup>3</sup> and H3K9ac correlated significantly with the expression of Gal-2. Furthermore, we detected an increase in H3K4me<sup>3</sup> and H3K9ac after the addition of Gal-2 to BeWo/HVT cells. Moreover, there was increased fusion of HVT cells after incubation with Gal-2. Gal-2 is associated with the histone modifications H3K4me<sup>3</sup> and H3K9ac in trophoblasts. Furthermore, syncytialisation increased after incubation with Gal-2. Therefore, we postulate that Gal-2 stimulates syncytialisation, possibly mediated by H3K4me<sup>3</sup> and H3K9ac. Since Gal-2, as well as H3K4me<sup>3</sup> and H3K9ac, are decreased in PE, the induction of Gal-2 might be a promising therapeutic target.https://www.mdpi.com/2218-273X/12/5/707preeclampsiahistone modificationH3K4me<sup>3</sup>H3K9acgalectin-2syncytialisation |
| spellingShingle | Laura Hahn Sarah Meister Mareike Mannewitz Susanne Beyer Stefanie Corradini Uwe Hasbargen Sven Mahner Udo Jeschke Thomas Kolben Alexander Burges Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia Biomolecules preeclampsia histone modification H3K4me<sup>3</sup> H3K9ac galectin-2 syncytialisation |
| title | Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia |
| title_full | Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia |
| title_fullStr | Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia |
| title_full_unstemmed | Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia |
| title_short | Gal-2 Increases H3K4me<sup>3</sup> and H3K9ac in Trophoblasts and Preeclampsia |
| title_sort | gal 2 increases h3k4me sup 3 sup and h3k9ac in trophoblasts and preeclampsia |
| topic | preeclampsia histone modification H3K4me<sup>3</sup> H3K9ac galectin-2 syncytialisation |
| url | https://www.mdpi.com/2218-273X/12/5/707 |
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