Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology
Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular metabolism, the optimization of metabolic pathways for maximal...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2018-06-01
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| Series: | Synthetic and Systems Biotechnology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405805X17301308 |
| _version_ | 1797204436240564224 |
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| author | Lihong Jiang Jiarun Zhao Jiazhang Lian Zhinan Xu |
| author_facet | Lihong Jiang Jiarun Zhao Jiazhang Lian Zhinan Xu |
| author_sort | Lihong Jiang |
| collection | DOAJ |
| description | Advances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular metabolism, the optimization of metabolic pathways for maximal production represents a grand challenge and an unavoidable barrier for metabolic engineering. Recently, cell-free protein synthesis system (CFPS) has been emerging as an enabling alternative to address challenges in biomanufacturing. This review summarizes the recent progresses of CFPS in rapid prototyping of biosynthetic pathways and genetic circuits (biosensors) to speed up design-build-test (DBT) cycles of metabolic engineering and synthetic biology. Keywords: Cell-free protein synthesis, Metabolic pathway optimization, Genetic circuits, Metabolic engineering, Synthetic biology |
| first_indexed | 2024-04-24T08:35:12Z |
| format | Article |
| id | doaj.art-0836c3ebf65b4b36aa87f6756b4a0e55 |
| institution | Directory Open Access Journal |
| issn | 2405-805X |
| language | English |
| last_indexed | 2024-04-24T08:35:12Z |
| publishDate | 2018-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Synthetic and Systems Biotechnology |
| spelling | doaj.art-0836c3ebf65b4b36aa87f6756b4a0e552024-04-16T17:33:09ZengKeAi Communications Co., Ltd.Synthetic and Systems Biotechnology2405-805X2018-06-01329096Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biologyLihong Jiang0Jiarun Zhao1Jiazhang Lian2Zhinan Xu3Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaKey Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaCorresponding author.; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaCorresponding author.; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, ChinaAdvances in metabolic engineering and synthetic biology have facilitated the manufacturing of many valuable-added compounds and commodity chemicals using microbial cell factories in the past decade. However, due to complexity of cellular metabolism, the optimization of metabolic pathways for maximal production represents a grand challenge and an unavoidable barrier for metabolic engineering. Recently, cell-free protein synthesis system (CFPS) has been emerging as an enabling alternative to address challenges in biomanufacturing. This review summarizes the recent progresses of CFPS in rapid prototyping of biosynthetic pathways and genetic circuits (biosensors) to speed up design-build-test (DBT) cycles of metabolic engineering and synthetic biology. Keywords: Cell-free protein synthesis, Metabolic pathway optimization, Genetic circuits, Metabolic engineering, Synthetic biologyhttp://www.sciencedirect.com/science/article/pii/S2405805X17301308 |
| spellingShingle | Lihong Jiang Jiarun Zhao Jiazhang Lian Zhinan Xu Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology Synthetic and Systems Biotechnology |
| title | Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology |
| title_full | Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology |
| title_fullStr | Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology |
| title_full_unstemmed | Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology |
| title_short | Cell-free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology |
| title_sort | cell free protein synthesis enabled rapid prototyping for metabolic engineering and synthetic biology |
| url | http://www.sciencedirect.com/science/article/pii/S2405805X17301308 |
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