Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy
BackgroundPrevious studies showed that patients with Graves’ orbitopathy (GO) had concomitant mucosal abnormality within the paranasal sinuses. It remains unknown whether the immunological reactions in sinus mucosa affect the orbit inflammation in GO.MethodsPatients with GO underwent sinus computed...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-02-01
|
| Series: | Frontiers in Endocrinology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2020.621321/full |
| _version_ | 1818342739965640704 |
|---|---|
| author | Yi Lu Yi Lu Yu Wu Yu Wu Yazhuo Huang Yazhuo Huang Sijie Fang Sijie Fang Yinwei Li Yinwei Li Jing Sun Jing Sun Huifang Zhou Huifang Zhou |
| author_facet | Yi Lu Yi Lu Yu Wu Yu Wu Yazhuo Huang Yazhuo Huang Sijie Fang Sijie Fang Yinwei Li Yinwei Li Jing Sun Jing Sun Huifang Zhou Huifang Zhou |
| author_sort | Yi Lu |
| collection | DOAJ |
| description | BackgroundPrevious studies showed that patients with Graves’ orbitopathy (GO) had concomitant mucosal abnormality within the paranasal sinuses. It remains unknown whether the immunological reactions in sinus mucosa affect the orbit inflammation in GO.MethodsPatients with GO underwent sinus computed tomography (CT) scans for sinus mucosal disease by two independent reviewers using the Lund-MacKay systems. Ethmoid mucosal samples were collected during orbital decompression surgeries for patients with GO and correction surgeries for patients with old orbital fractures as controls. Histological analysis and immunofluorescence were performed in all sinus mucosa tissues. Flow cytometry analysis was used to examine the immunological features of sinus mucosa in both GO and control groups.ResultsImmunohistochemistry showed that the paranasal sinus mucosa of patients with GO grew swelling, with goblet cell and small vessel proliferation, endothelial cell swelling, and inflammatory cell infiltration. The number of T helper (Th)1, Th17, and gamma-delta T cells in nasal sinus mucosa of patients with GO increased significantly compared with those from controls. Further, the proportion of Th1 cells was significantly correlated with clinical activity score. In addition, there was a decreased number of regulatory T cells in patients with GO. The number of Th2 cells showed no significant difference between the two groups. Finally, the proportion of interleukin-22-producing cell subsets in gamma-delta T cells of patients with GO was significantly increased compared with those from controls.ConclusionsOur observations illustrated a potential pathogenic role of mucosal-infiltrating T cells, which may have the possibility to aggravate inflammatory responses in GO. |
| first_indexed | 2024-12-13T16:19:29Z |
| format | Article |
| id | doaj.art-08379392b2034e86b79fccfba1474ea6 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-13T16:19:29Z |
| publishDate | 2021-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-08379392b2034e86b79fccfba1474ea62022-12-21T23:38:45ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-02-011110.3389/fendo.2020.621321621321Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ OrbitopathyYi Lu0Yi Lu1Yu Wu2Yu Wu3Yazhuo Huang4Yazhuo Huang5Sijie Fang6Sijie Fang7Yinwei Li8Yinwei Li9Jing Sun10Jing Sun11Huifang Zhou12Huifang Zhou13Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaDepartment of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaDepartment of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaDepartment of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaDepartment of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaDepartment of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaDepartment of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, ChinaShanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai Ninth People's Hospital, Shanghai, ChinaBackgroundPrevious studies showed that patients with Graves’ orbitopathy (GO) had concomitant mucosal abnormality within the paranasal sinuses. It remains unknown whether the immunological reactions in sinus mucosa affect the orbit inflammation in GO.MethodsPatients with GO underwent sinus computed tomography (CT) scans for sinus mucosal disease by two independent reviewers using the Lund-MacKay systems. Ethmoid mucosal samples were collected during orbital decompression surgeries for patients with GO and correction surgeries for patients with old orbital fractures as controls. Histological analysis and immunofluorescence were performed in all sinus mucosa tissues. Flow cytometry analysis was used to examine the immunological features of sinus mucosa in both GO and control groups.ResultsImmunohistochemistry showed that the paranasal sinus mucosa of patients with GO grew swelling, with goblet cell and small vessel proliferation, endothelial cell swelling, and inflammatory cell infiltration. The number of T helper (Th)1, Th17, and gamma-delta T cells in nasal sinus mucosa of patients with GO increased significantly compared with those from controls. Further, the proportion of Th1 cells was significantly correlated with clinical activity score. In addition, there was a decreased number of regulatory T cells in patients with GO. The number of Th2 cells showed no significant difference between the two groups. Finally, the proportion of interleukin-22-producing cell subsets in gamma-delta T cells of patients with GO was significantly increased compared with those from controls.ConclusionsOur observations illustrated a potential pathogenic role of mucosal-infiltrating T cells, which may have the possibility to aggravate inflammatory responses in GO.https://www.frontiersin.org/articles/10.3389/fendo.2020.621321/fullimmuneT cellsparanasal sinusmucosaGraves’ orbitopathy |
| spellingShingle | Yi Lu Yi Lu Yu Wu Yu Wu Yazhuo Huang Yazhuo Huang Sijie Fang Sijie Fang Yinwei Li Yinwei Li Jing Sun Jing Sun Huifang Zhou Huifang Zhou Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy Frontiers in Endocrinology immune T cells paranasal sinus mucosa Graves’ orbitopathy |
| title | Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy |
| title_full | Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy |
| title_fullStr | Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy |
| title_full_unstemmed | Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy |
| title_short | Immunological Features of Paranasal Sinus Mucosa in Patients with Graves’ Orbitopathy |
| title_sort | immunological features of paranasal sinus mucosa in patients with graves orbitopathy |
| topic | immune T cells paranasal sinus mucosa Graves’ orbitopathy |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2020.621321/full |
| work_keys_str_mv | AT yilu immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yilu immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yuwu immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yuwu immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yazhuohuang immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yazhuohuang immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT sijiefang immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT sijiefang immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yinweili immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT yinweili immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT jingsun immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT jingsun immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT huifangzhou immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy AT huifangzhou immunologicalfeaturesofparanasalsinusmucosainpatientswithgravesorbitopathy |