Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc
Abstract Background Non-small cell lung cancer (NSCLC) causes numerous deaths worldwide. however, biomarkers for NSCLC prognosis are scarce for its heterogeneity. Proteins containing the RING finger domain RING finger protein 180 (RNF180) is a key mediator for ubiquitination, which controls cell cyc...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | World Journal of Surgical Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12957-022-02599-x |
| _version_ | 1818240782606270464 |
|---|---|
| author | Yi Ding Yi Lu Xinjie Xie Lei Cao Shiying Zheng |
| author_facet | Yi Ding Yi Lu Xinjie Xie Lei Cao Shiying Zheng |
| author_sort | Yi Ding |
| collection | DOAJ |
| description | Abstract Background Non-small cell lung cancer (NSCLC) causes numerous deaths worldwide. however, biomarkers for NSCLC prognosis are scarce for its heterogeneity. Proteins containing the RING finger domain RING finger protein 180 (RNF180) is a key mediator for ubiquitination, which controls cell cycle and regulates progression in certain human tumors. However, the detailed function of RNF180 in NSCLC remains unclear. In the present study, we aimed to investigate the role of RNF180 and its molecule network in NSCLC. Methods Quantitative real-time polymerase chain reaction and immunohistochemical staining were used to analyze RNF180 levels. RNA interference and lentiviral-mediated vector transfections were performed to silence and overexpress RNF180 in NSCLC cells. Furthermore, Cell Counting Kit-8 was used for assessing biological function of RNF180 in cell proliferation and a xenograft model for examining its function in vivo. The activity of glycolysis was determined by examining the level of the extracellular acidification rate (ECAR). Results RNF180 expression decreased in NSCLC tissues, and its expression was positively correlated with the survival rate of patients with NSCLC. Moreover, RNF180 overexpression suppressed the proliferation and glycolytic activities in NSCLC cells and restricted its tumorigenicity in vivo. Furthermore, RNF180 silencing promoted the proliferation and glycolysis metabolism of NSCLC cells, whereas C-myc inhibitor disrupted these effects. The underlying anti-oncogene of RNF180 involved in C-myc downregulation via ubiquitin-dependent degradation. Conclusions Together, these results firstly indicated the anti-tumor properties of RNF180 and its correlation with NSCLC progression, thereby endorsing the potential role of RNF180 as an efficient prognostic biomarker for tumor recurrence. |
| first_indexed | 2024-12-12T13:18:55Z |
| format | Article |
| id | doaj.art-0839a12f332d49759871f771e0b4e31b |
| institution | Directory Open Access Journal |
| issn | 1477-7819 |
| language | English |
| last_indexed | 2024-12-12T13:18:55Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | World Journal of Surgical Oncology |
| spelling | doaj.art-0839a12f332d49759871f771e0b4e31b2022-12-22T00:23:20ZengBMCWorld Journal of Surgical Oncology1477-78192022-05-0120111010.1186/s12957-022-02599-xRing finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-mycYi Ding0Yi Lu1Xinjie Xie2Lei Cao3Shiying Zheng4Department of Thoracic Surgery, The First Affiliated Hospital of Soochow UniversityDepartment of Thoracic Surgery, Shanghai Pudong New Area People’s Hospital, Shanghai University of Medicine and Health SciencesDepartment of Thoracic Surgery, Shanghai Pudong New Area People’s Hospital, Shanghai University of Medicine and Health SciencesDepartment of Pathology, Shanghai Pudong New Area People’s Hospital, Shanghai University of Medicine and Health SciencesDepartment of Thoracic Surgery, The First Affiliated Hospital of Soochow UniversityAbstract Background Non-small cell lung cancer (NSCLC) causes numerous deaths worldwide. however, biomarkers for NSCLC prognosis are scarce for its heterogeneity. Proteins containing the RING finger domain RING finger protein 180 (RNF180) is a key mediator for ubiquitination, which controls cell cycle and regulates progression in certain human tumors. However, the detailed function of RNF180 in NSCLC remains unclear. In the present study, we aimed to investigate the role of RNF180 and its molecule network in NSCLC. Methods Quantitative real-time polymerase chain reaction and immunohistochemical staining were used to analyze RNF180 levels. RNA interference and lentiviral-mediated vector transfections were performed to silence and overexpress RNF180 in NSCLC cells. Furthermore, Cell Counting Kit-8 was used for assessing biological function of RNF180 in cell proliferation and a xenograft model for examining its function in vivo. The activity of glycolysis was determined by examining the level of the extracellular acidification rate (ECAR). Results RNF180 expression decreased in NSCLC tissues, and its expression was positively correlated with the survival rate of patients with NSCLC. Moreover, RNF180 overexpression suppressed the proliferation and glycolytic activities in NSCLC cells and restricted its tumorigenicity in vivo. Furthermore, RNF180 silencing promoted the proliferation and glycolysis metabolism of NSCLC cells, whereas C-myc inhibitor disrupted these effects. The underlying anti-oncogene of RNF180 involved in C-myc downregulation via ubiquitin-dependent degradation. Conclusions Together, these results firstly indicated the anti-tumor properties of RNF180 and its correlation with NSCLC progression, thereby endorsing the potential role of RNF180 as an efficient prognostic biomarker for tumor recurrence.https://doi.org/10.1186/s12957-022-02599-xNon-small cell lung cancerRING finger protein 180C-mycCell proliferationGlycolysisUbiquitination |
| spellingShingle | Yi Ding Yi Lu Xinjie Xie Lei Cao Shiying Zheng Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc World Journal of Surgical Oncology Non-small cell lung cancer RING finger protein 180 C-myc Cell proliferation Glycolysis Ubiquitination |
| title | Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc |
| title_full | Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc |
| title_fullStr | Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc |
| title_full_unstemmed | Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc |
| title_short | Ring finger protein 180 suppresses cell proliferation and energy metabolism of non-small cell lung cancer through downregulating C-myc |
| title_sort | ring finger protein 180 suppresses cell proliferation and energy metabolism of non small cell lung cancer through downregulating c myc |
| topic | Non-small cell lung cancer RING finger protein 180 C-myc Cell proliferation Glycolysis Ubiquitination |
| url | https://doi.org/10.1186/s12957-022-02599-x |
| work_keys_str_mv | AT yiding ringfingerprotein180suppressescellproliferationandenergymetabolismofnonsmallcelllungcancerthroughdownregulatingcmyc AT yilu ringfingerprotein180suppressescellproliferationandenergymetabolismofnonsmallcelllungcancerthroughdownregulatingcmyc AT xinjiexie ringfingerprotein180suppressescellproliferationandenergymetabolismofnonsmallcelllungcancerthroughdownregulatingcmyc AT leicao ringfingerprotein180suppressescellproliferationandenergymetabolismofnonsmallcelllungcancerthroughdownregulatingcmyc AT shiyingzheng ringfingerprotein180suppressescellproliferationandenergymetabolismofnonsmallcelllungcancerthroughdownregulatingcmyc |