Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis
Katokkon pepper plant is originated from Toraja, South Sulawesi, Indonesia. This endemic pepper variety has unique hot and spicy characters with a distinctive bell pepper aroma, and aesthetic shape similar to paprika, but with smaller size. This research was conducted to identify ploidy level and pl...
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| Format: | Article |
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Universitas Brawijaya
2023-06-01
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| Series: | AGRIVITA Journal of Agricultural Science |
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| Online Access: | https://agrivita.ub.ac.id/index.php/agrivita/article/view/3633 |
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| author | Rinaldi Sjahril Muh. Riadi Ifayanti Ridwan Saleh Novitasari Novitasari Ernitha A. Galla Kasmiati Kasmiati A. R. Trisnawaty Nurhaya J. Panga |
| author_facet | Rinaldi Sjahril Muh. Riadi Ifayanti Ridwan Saleh Novitasari Novitasari Ernitha A. Galla Kasmiati Kasmiati A. R. Trisnawaty Nurhaya J. Panga |
| author_sort | Rinaldi Sjahril |
| collection | DOAJ |
| description | Katokkon pepper plant is originated from Toraja, South Sulawesi, Indonesia. This endemic pepper variety has unique hot and spicy characters with a distinctive bell pepper aroma, and aesthetic shape similar to paprika, but with smaller size. This research was conducted to identify ploidy level and plant morphology of colchicine induced Katokkon pepper at Laboratory of Plant Reproduction Bioscience and Biotechnology, Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin and experimental field of Agricultural Extension System Vocational High School Santo Paulus Tana Toraja (S 03°04’177” E 119°51’526”). Two weeks old seedlings were immersed for 4.5 hours in colchicine concentration (0.00%, 0.0125%, 0.025%, 0.05% and 0.10%). Flow cytometry analysis was carried out using Partec Cy-Flow SpaceTM. Result showed that colchicine concentration (0.025%, 0.05% and 0.1%) produced mixoploid plants with two set of chromosomes (2n=24, 4n=48). This study also found morphological differences between mixoploids plants induced by 0.025%, 0.05% and 0.1% colchicine and diploid plants (0% and 0.0125% colchicine) during first two juvenile leaves phase. However, this difference did not occur further, and eventually morphology of adult mixoploid plant was not significantly different from control (diploid), which concurred to grading mixoploid grade 2. The mixoploid plants analyzed consist of higher diploids cells than tetraploid. |
| first_indexed | 2024-03-11T20:31:55Z |
| format | Article |
| id | doaj.art-083a157c17cc4c39ad672790684cbe33 |
| institution | Directory Open Access Journal |
| issn | 0126-0537 2477-8516 |
| language | English |
| last_indexed | 2024-03-11T20:31:55Z |
| publishDate | 2023-06-01 |
| publisher | Universitas Brawijaya |
| record_format | Article |
| series | AGRIVITA Journal of Agricultural Science |
| spelling | doaj.art-083a157c17cc4c39ad672790684cbe332023-10-02T07:41:38ZengUniversitas BrawijayaAGRIVITA Journal of Agricultural Science0126-05372477-85162023-06-0145228829510.17503/agrivita.v45i2.3633742Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry AnalysisRinaldi Sjahril0Muh. Riadi1Ifayanti Ridwan Saleh2Novitasari Novitasari3Ernitha A. Galla4Kasmiati Kasmiati5A. R. Trisnawaty6Nurhaya J. Panga7Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin, Makassar, South Sulawesi, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Universitas Hasanuddin, Makassar, South Sulawesi, IndonesiaDepartment of Agronomy, Faculty of Agriculture, Universitas Hasanuddin, Makassar, South Sulawesi, IndonesiaStudy Program of Magister Agrotechnology, Faculty of Agriculture, Universitas Hasanuddin, Makassar, South Sulawesi, IndonesiaFaculty of Agriculture, Universitas Kristen Indonesia Toraja, Makale, Tana Toraja, South Sulawesi, IndonesiaLaboratory of Bioscience and Plant Reproduction Biotechnology, Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin, Makassar, South Sulawesi, IndonesiaDepartment of Agrotechnology, Faculty of Science and Technology, Universitas Muhammadiyah Sidenreng Rappang, Sidenreng Rappang, South Sulawesi, IndonesiaDepartment of Agrotechnology, Faculty of Agriculture, Universitas Musamus, Merauke, Papua, IndonesiaKatokkon pepper plant is originated from Toraja, South Sulawesi, Indonesia. This endemic pepper variety has unique hot and spicy characters with a distinctive bell pepper aroma, and aesthetic shape similar to paprika, but with smaller size. This research was conducted to identify ploidy level and plant morphology of colchicine induced Katokkon pepper at Laboratory of Plant Reproduction Bioscience and Biotechnology, Department of Agronomy, Faculty of Agriculture, Universitas Hasanuddin and experimental field of Agricultural Extension System Vocational High School Santo Paulus Tana Toraja (S 03°04’177” E 119°51’526”). Two weeks old seedlings were immersed for 4.5 hours in colchicine concentration (0.00%, 0.0125%, 0.025%, 0.05% and 0.10%). Flow cytometry analysis was carried out using Partec Cy-Flow SpaceTM. Result showed that colchicine concentration (0.025%, 0.05% and 0.1%) produced mixoploid plants with two set of chromosomes (2n=24, 4n=48). This study also found morphological differences between mixoploids plants induced by 0.025%, 0.05% and 0.1% colchicine and diploid plants (0% and 0.0125% colchicine) during first two juvenile leaves phase. However, this difference did not occur further, and eventually morphology of adult mixoploid plant was not significantly different from control (diploid), which concurred to grading mixoploid grade 2. The mixoploid plants analyzed consist of higher diploids cells than tetraploid.https://agrivita.ub.ac.id/index.php/agrivita/article/view/3633colchicineflow cytometrykatokkon peppertoraja |
| spellingShingle | Rinaldi Sjahril Muh. Riadi Ifayanti Ridwan Saleh Novitasari Novitasari Ernitha A. Galla Kasmiati Kasmiati A. R. Trisnawaty Nurhaya J. Panga Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis AGRIVITA Journal of Agricultural Science colchicine flow cytometry katokkon pepper toraja |
| title | Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis |
| title_full | Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis |
| title_fullStr | Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis |
| title_full_unstemmed | Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis |
| title_short | Katokkon Pepper (Capsicum chinense Jacq.) Ploidy Determination by Morphological Characteristic and Flow Cytometry Analysis |
| title_sort | katokkon pepper capsicum chinense jacq ploidy determination by morphological characteristic and flow cytometry analysis |
| topic | colchicine flow cytometry katokkon pepper toraja |
| url | https://agrivita.ub.ac.id/index.php/agrivita/article/view/3633 |
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