Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028
The emergence of multidrug-resistant microorganisms represents a global challenge that has led to a search for new antimicrobial compounds. Essential oils (EOs) from medicinal aromatic plants are a potential alternative for conventional antibiotics. In this study, the antimicrobial and anti-biofilm...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/10/10/1191 |
| _version_ | 1797515468794232832 |
|---|---|
| author | Yuliany Guillín Marlon Cáceres Rodrigo Torres Elena Stashenko Claudia Ortiz |
| author_facet | Yuliany Guillín Marlon Cáceres Rodrigo Torres Elena Stashenko Claudia Ortiz |
| author_sort | Yuliany Guillín |
| collection | DOAJ |
| description | The emergence of multidrug-resistant microorganisms represents a global challenge that has led to a search for new antimicrobial compounds. Essential oils (EOs) from medicinal aromatic plants are a potential alternative for conventional antibiotics. In this study, the antimicrobial and anti-biofilm potential of 15 EOs was evaluated on planktonic and biofilm-associated cells of <i>Salmonella enterica</i> serovar Enteritidis ATCC 13076 (<i>S. enteritidis</i>) and <i>Salmonella enterica</i> serovar Typhimurium ATCC 14028 (<i>S. typhimurium</i>). In total, 4 out of 15 EOs showed antimicrobial activity and 6 EOs showed anti-biofilm activity against both strains. The EO from the <i>Lippia origanoides</i> chemotype thymol-carvacrol II (LTC II) presented the lowest minimum inhibitory concentration (MIC<sub>50</sub> = 0.37 mg mL<sup>−1</sup>) and minimum bactericidal concentration (MBC = 0.75 mg mL<sup>−1</sup>) values. This EO also presented the highest percentage of biofilm inhibition (>65%) on both microorganisms, which could be confirmed by scanning electron microscopy (SEM) images. Transcriptional analysis showed significant changes in the expression of the genes related to quorum sensing and the formation of the biofilm. EOs could inhibit the expression of genes involved in the quorum sensing mechanism (<i>luxR, luxS</i>, <i>qseB</i>, <i>sdiA</i>) and biofilm formation (<i>csgA</i>, <i>csgB,</i> <i>csgD</i>, <i>flhD</i>, <i>fliZ</i>, and <i>motB</i>), indicating their potential use as anti-biofilm antimicrobial agents. However, further studies are needed to elucidate the action mechanisms of essential oils on the bacterial cells under study. |
| first_indexed | 2024-03-10T06:45:55Z |
| format | Article |
| id | doaj.art-083bb97e9e104c4eb24ffb0e155ace7a |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T06:45:55Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-083bb97e9e104c4eb24ffb0e155ace7a2023-11-22T17:13:30ZengMDPI AGAntibiotics2079-63822021-10-011010119110.3390/antibiotics10101191Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028Yuliany Guillín0Marlon Cáceres1Rodrigo Torres2Elena Stashenko3Claudia Ortiz4Escuela de Biología, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaEscuela de Medicina, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaGrupo de Investigación en Bioquímica y Microbiología, Bucaramanga 680002, ColombiaCentro de Cromatografía y Espectrometría de Masas, CROM-MASS-CENIVAM, Facultad de Ciencias, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaEscuela de Microbiología y Bioanálisis, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaThe emergence of multidrug-resistant microorganisms represents a global challenge that has led to a search for new antimicrobial compounds. Essential oils (EOs) from medicinal aromatic plants are a potential alternative for conventional antibiotics. In this study, the antimicrobial and anti-biofilm potential of 15 EOs was evaluated on planktonic and biofilm-associated cells of <i>Salmonella enterica</i> serovar Enteritidis ATCC 13076 (<i>S. enteritidis</i>) and <i>Salmonella enterica</i> serovar Typhimurium ATCC 14028 (<i>S. typhimurium</i>). In total, 4 out of 15 EOs showed antimicrobial activity and 6 EOs showed anti-biofilm activity against both strains. The EO from the <i>Lippia origanoides</i> chemotype thymol-carvacrol II (LTC II) presented the lowest minimum inhibitory concentration (MIC<sub>50</sub> = 0.37 mg mL<sup>−1</sup>) and minimum bactericidal concentration (MBC = 0.75 mg mL<sup>−1</sup>) values. This EO also presented the highest percentage of biofilm inhibition (>65%) on both microorganisms, which could be confirmed by scanning electron microscopy (SEM) images. Transcriptional analysis showed significant changes in the expression of the genes related to quorum sensing and the formation of the biofilm. EOs could inhibit the expression of genes involved in the quorum sensing mechanism (<i>luxR, luxS</i>, <i>qseB</i>, <i>sdiA</i>) and biofilm formation (<i>csgA</i>, <i>csgB,</i> <i>csgD</i>, <i>flhD</i>, <i>fliZ</i>, and <i>motB</i>), indicating their potential use as anti-biofilm antimicrobial agents. However, further studies are needed to elucidate the action mechanisms of essential oils on the bacterial cells under study.https://www.mdpi.com/2079-6382/10/10/1191essential oilsbiofilm<i>Salmonella</i>antimicrobialsRT-qPCR |
| spellingShingle | Yuliany Guillín Marlon Cáceres Rodrigo Torres Elena Stashenko Claudia Ortiz Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028 Antibiotics essential oils biofilm <i>Salmonella</i> antimicrobials RT-qPCR |
| title | Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028 |
| title_full | Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028 |
| title_fullStr | Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028 |
| title_full_unstemmed | Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028 |
| title_short | Effect of Essential Oils on the Inhibition of Biofilm and Quorum Sensing in <i>Salmonella enteritidis</i> 13076 and <i>Salmonella typhimurium</i> 14028 |
| title_sort | effect of essential oils on the inhibition of biofilm and quorum sensing in i salmonella enteritidis i 13076 and i salmonella typhimurium i 14028 |
| topic | essential oils biofilm <i>Salmonella</i> antimicrobials RT-qPCR |
| url | https://www.mdpi.com/2079-6382/10/10/1191 |
| work_keys_str_mv | AT yulianyguillin effectofessentialoilsontheinhibitionofbiofilmandquorumsensinginisalmonellaenteritidisi13076andisalmonellatyphimuriumi14028 AT marloncaceres effectofessentialoilsontheinhibitionofbiofilmandquorumsensinginisalmonellaenteritidisi13076andisalmonellatyphimuriumi14028 AT rodrigotorres effectofessentialoilsontheinhibitionofbiofilmandquorumsensinginisalmonellaenteritidisi13076andisalmonellatyphimuriumi14028 AT elenastashenko effectofessentialoilsontheinhibitionofbiofilmandquorumsensinginisalmonellaenteritidisi13076andisalmonellatyphimuriumi14028 AT claudiaortiz effectofessentialoilsontheinhibitionofbiofilmandquorumsensinginisalmonellaenteritidisi13076andisalmonellatyphimuriumi14028 |