The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus <i>Conidiobolus coronatus</i>. In this study, <i>Sarcophaga argy...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/11/970 |
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| author | Agata Kaczmarek Mieczysława Irena Boguś |
| author_facet | Agata Kaczmarek Mieczysława Irena Boguś |
| author_sort | Agata Kaczmarek |
| collection | DOAJ |
| description | The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus <i>Conidiobolus coronatus</i>. In this study, <i>Sarcophaga argyrostoma</i> flies were exposed to sporulating <i>C. coronatus</i> colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with <i>C. coronatus</i> exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi. |
| first_indexed | 2024-03-10T05:24:17Z |
| format | Article |
| id | doaj.art-083ebd7306494843bf3026cb0426c03a |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T05:24:17Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-083ebd7306494843bf3026cb0426c03a2023-11-22T23:46:32ZengMDPI AGInsects2075-44502021-10-01121197010.3390/insects12110970The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>Agata Kaczmarek0Mieczysława Irena Boguś1Witold Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, PolandWitold Stefański Institute of Parasitology, Polish Academy of Sciences, Twarda 51/55, 00-818 Warsaw, PolandThe chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus <i>Conidiobolus coronatus</i>. In this study, <i>Sarcophaga argyrostoma</i> flies were exposed to sporulating <i>C. coronatus</i> colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with <i>C. coronatus</i> exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi.https://www.mdpi.com/2075-4450/12/11/970cuticleentomopathogensfree fatty acidsfungal infectionpolyunsaturated fatty acids |
| spellingShingle | Agata Kaczmarek Mieczysława Irena Boguś The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i> Insects cuticle entomopathogens free fatty acids fungal infection polyunsaturated fatty acids |
| title | The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i> |
| title_full | The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i> |
| title_fullStr | The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i> |
| title_full_unstemmed | The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i> |
| title_short | The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i> |
| title_sort | impact of the entomopathogenic fungus i conidiobolus coronatus i on the free fatty acid profile of the flesh fly i sarcophaga argyrostoma i |
| topic | cuticle entomopathogens free fatty acids fungal infection polyunsaturated fatty acids |
| url | https://www.mdpi.com/2075-4450/12/11/970 |
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