In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium
This study evaluates in vitro tolerance of white poplar genotypes to the presence of lead in an acidic rooting medium. Lead was supplemented in form of Pb(NO3)2, in the following concentrations: 0 M (Control), 10-6 M, 10-5 M, 10-4 M and 10-3 M. After four weeks of cultivation, the following characte...
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| Format: | Article |
| Language: | English |
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Croatian Forest Research Institute
2020-01-01
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| Series: | South-East European Forestry |
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| Online Access: | https://hrcak.srce.hr/file/361239 |
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| author | Branislav Kovačević Gordana Tišma Nataša Nikolić Marina Milović Vanja Vuksanović Saša Orlović |
| author_facet | Branislav Kovačević Gordana Tišma Nataša Nikolić Marina Milović Vanja Vuksanović Saša Orlović |
| author_sort | Branislav Kovačević |
| collection | DOAJ |
| description | This study evaluates in vitro tolerance of white poplar genotypes to the presence of lead in an acidic rooting medium. Lead was supplemented in form of Pb(NO3)2, in the following concentrations: 0 M (Control), 10-6 M, 10-5 M, 10-4 M and 10-3 M. After four weeks of cultivation, the following characters were measured: number of roots, the length of the longest root, the shoot height, dry root mass and dry shoot mass, and parameters related to photosynthetic pigments: content of chlorophyll a, b, a+b, and of carotenoids in fresh mass, as well as chlorophyll a/b ratio. For further statistical analysis, tolerance indices by Turner and Marshal (TI) were calculated for each measured character. The strongest inhibitory effect was achieved on the medium with 10-4 M Pb(NO3)2, but the best differentiation between genotypes was achieved on the medium with 10-5 M Pb(NO3)2. The highest tolerance indices for the length of the longest root and shoot height had genotypes L-12 and LBM, and for root and shoot dry mass genotypes LCM and L-12. There were no significant differences between genotypes in tolerance indices by any of the examined photosynthetic parameters. The obtained results suggest that both low pH and the presence of citric acid as chelating agent improved evaluation of lead tolerance in comparison with the results obtained on media with standard pH and without citric acid in similar studies. In vitro tests on acidic medium with citric acid can efficiently differentiate examined genotypes for lead tolerance, which could be important for their use in phytoextraction projects, especially on acidic soils. |
| first_indexed | 2024-04-24T09:16:38Z |
| format | Article |
| id | doaj.art-083ff0e6086c4fa2b4daec28b2e5fd70 |
| institution | Directory Open Access Journal |
| issn | 1847-6481 1849-0891 |
| language | English |
| last_indexed | 2024-04-24T09:16:38Z |
| publishDate | 2020-01-01 |
| publisher | Croatian Forest Research Institute |
| record_format | Article |
| series | South-East European Forestry |
| spelling | doaj.art-083ff0e6086c4fa2b4daec28b2e5fd702024-04-15T16:39:14ZengCroatian Forest Research InstituteSouth-East European Forestry1847-64811849-08912020-01-0111215316010.15177/seefor.20-18In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic MediumBranislav Kovačević0Gordana Tišma1Nataša Nikolić2Marina Milović3Vanja Vuksanović4Saša Orlović5University of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13, RS-21000 Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Sciences, Trg Dositeja Obradovića 3, RS-21001 Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Sciences, Trg Dositeja Obradovića 3, RS-21001 Novi Sad, SerbiaUniversity of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13, RS-21000 Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, RS-21002 Novi Sad, SerbiaUniversity of Novi Sad, Institute of Lowland Forestry and Environment, Antona Čehova 13, RS-21000 Novi Sad, SerbiaThis study evaluates in vitro tolerance of white poplar genotypes to the presence of lead in an acidic rooting medium. Lead was supplemented in form of Pb(NO3)2, in the following concentrations: 0 M (Control), 10-6 M, 10-5 M, 10-4 M and 10-3 M. After four weeks of cultivation, the following characters were measured: number of roots, the length of the longest root, the shoot height, dry root mass and dry shoot mass, and parameters related to photosynthetic pigments: content of chlorophyll a, b, a+b, and of carotenoids in fresh mass, as well as chlorophyll a/b ratio. For further statistical analysis, tolerance indices by Turner and Marshal (TI) were calculated for each measured character. The strongest inhibitory effect was achieved on the medium with 10-4 M Pb(NO3)2, but the best differentiation between genotypes was achieved on the medium with 10-5 M Pb(NO3)2. The highest tolerance indices for the length of the longest root and shoot height had genotypes L-12 and LBM, and for root and shoot dry mass genotypes LCM and L-12. There were no significant differences between genotypes in tolerance indices by any of the examined photosynthetic parameters. The obtained results suggest that both low pH and the presence of citric acid as chelating agent improved evaluation of lead tolerance in comparison with the results obtained on media with standard pH and without citric acid in similar studies. In vitro tests on acidic medium with citric acid can efficiently differentiate examined genotypes for lead tolerance, which could be important for their use in phytoextraction projects, especially on acidic soils.https://hrcak.srce.hr/file/361239Populus albaheavy metalslow pHmicrowave sterilization |
| spellingShingle | Branislav Kovačević Gordana Tišma Nataša Nikolić Marina Milović Vanja Vuksanović Saša Orlović In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium South-East European Forestry Populus alba heavy metals low pH microwave sterilization |
| title | In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium |
| title_full | In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium |
| title_fullStr | In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium |
| title_full_unstemmed | In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium |
| title_short | In Vitro Lead Tolerance Testing in White Poplar Genotypes on Acidic Medium |
| title_sort | in vitro lead tolerance testing in white poplar genotypes on acidic medium |
| topic | Populus alba heavy metals low pH microwave sterilization |
| url | https://hrcak.srce.hr/file/361239 |
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