A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L.
Baculoviruses can persist in insect host organisms after infection and may be vertically transmitted to the next generation, in which they may be reactivated. The goal of the present study was to compare the efficiency of the vertical transmission of high- and low-virulence strains and the subsequen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/7/455 |
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| author | Yuriy B. Akhanaev Irina A. Belousova Darya A. Lebedeva Sergey V. Pavlushin Vyacheslav V. Martemyanov |
| author_facet | Yuriy B. Akhanaev Irina A. Belousova Darya A. Lebedeva Sergey V. Pavlushin Vyacheslav V. Martemyanov |
| author_sort | Yuriy B. Akhanaev |
| collection | DOAJ |
| description | Baculoviruses can persist in insect host organisms after infection and may be vertically transmitted to the next generation, in which they may be reactivated. The goal of the present study was to compare the efficiency of the vertical transmission of high- and low-virulence strains and the subsequent reactivation of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) in the offspring of <i>Lymantria dispar</i> L. adults who survived after viral infection. As a result of parental infection, the fecundity of survived females, pupae weight, and fertility were significantly different compared to the untreated insects. However, differences in these parameters between high- and low-virulence strains were not observed. The prevalence of virus strains in the offspring measured by quantitative polymerase chain reaction also did not differ. When the larvae reached the fourth instar, they were starved to activate the vertically transmitted virus. The frequency of virus activation in the experiment was not dependent on the virulence of the virus strains. These results are helpful for understanding the strategy of virus survival in nature and for the selection of the most effective strains with transgenerational effects in the years following pest treatment. |
| first_indexed | 2024-03-10T18:21:33Z |
| format | Article |
| id | doaj.art-0841ad25a6de46159bffa585e25c2b84 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T18:21:33Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-0841ad25a6de46159bffa585e25c2b842023-11-20T07:16:41ZengMDPI AGInsects2075-44502020-07-0111745510.3390/insects11070455A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L.Yuriy B. Akhanaev0Irina A. Belousova1Darya A. Lebedeva2Sergey V. Pavlushin3Vyacheslav V. Martemyanov4Laboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk 630091, RussiaLaboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk 630091, RussiaLaboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk 630091, RussiaLaboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk 630091, RussiaLaboratory of Ecological Physiology, Institute of Systematics and Ecology of Animals SB RAS, Frunze str. 11, Novosibirsk 630091, RussiaBaculoviruses can persist in insect host organisms after infection and may be vertically transmitted to the next generation, in which they may be reactivated. The goal of the present study was to compare the efficiency of the vertical transmission of high- and low-virulence strains and the subsequent reactivation of Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV) in the offspring of <i>Lymantria dispar</i> L. adults who survived after viral infection. As a result of parental infection, the fecundity of survived females, pupae weight, and fertility were significantly different compared to the untreated insects. However, differences in these parameters between high- and low-virulence strains were not observed. The prevalence of virus strains in the offspring measured by quantitative polymerase chain reaction also did not differ. When the larvae reached the fourth instar, they were starved to activate the vertically transmitted virus. The frequency of virus activation in the experiment was not dependent on the virulence of the virus strains. These results are helpful for understanding the strategy of virus survival in nature and for the selection of the most effective strains with transgenerational effects in the years following pest treatment.https://www.mdpi.com/2075-4450/11/7/455Lymantria disparbaculovirusvertical transmissioncovert infectionreactivation |
| spellingShingle | Yuriy B. Akhanaev Irina A. Belousova Darya A. Lebedeva Sergey V. Pavlushin Vyacheslav V. Martemyanov A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L. Insects Lymantria dispar baculovirus vertical transmission covert infection reactivation |
| title | A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L. |
| title_full | A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L. |
| title_fullStr | A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L. |
| title_full_unstemmed | A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L. |
| title_short | A Comparison of the Vertical Transmission of High- and Low-Virulence Nucleopolyhedrovirus Strains in <i>Lymantria Dispar</i> L. |
| title_sort | comparison of the vertical transmission of high and low virulence nucleopolyhedrovirus strains in i lymantria dispar i l |
| topic | Lymantria dispar baculovirus vertical transmission covert infection reactivation |
| url | https://www.mdpi.com/2075-4450/11/7/455 |
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