CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS
The paper presents experimental study results of the cytopathic effect of peste de petits ruminants virus on a goat gonad continuous cell line (YaDK-04). The interaction of peste de petits ruminants virus with cells at different stages of its reproduction was shown using a combination of phase-contr...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2019-06-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/414 |
| _version_ | 1797224848657743872 |
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| author | B. L. Manin L. V. Malakhova A. B. Sarbasov N. V. Moroz |
| author_facet | B. L. Manin L. V. Malakhova A. B. Sarbasov N. V. Moroz |
| author_sort | B. L. Manin |
| collection | DOAJ |
| description | The paper presents experimental study results of the cytopathic effect of peste de petits ruminants virus on a goat gonad continuous cell line (YaDK-04). The interaction of peste de petits ruminants virus with cells at different stages of its reproduction was shown using a combination of phase-contrast and luminescent microscopy. It was found that at the initial stage of interaction (20–24 hours) the cells became rounded and de-adhered, and the monolayer was partially loosened. On day 2 post reproduction the most part of the culture monolayer affected by the virus began to destruct, and the cell nuclei were displaced to periphery. At the terminal stage (72 hours) the destruction of monolayer cells and cytoplasmic matrix, deformation and partial lysis of the nuclei and cytoplasm, aggregation of detritus occurred. At the final stage of reproduction (96 hours) the peste de petits ruminants virus diffused into the culture medium, the fluorescence in the yellow spectrum decreased significantly, but the virus titer reached 6.89 lg TCD50/cm3. |
| first_indexed | 2024-03-09T02:48:53Z |
| format | Article |
| id | doaj.art-08421f2b8e0e4331b69e4cd51db24669 |
| institution | Directory Open Access Journal |
| issn | 2304-196X 2658-6959 |
| language | English |
| last_indexed | 2024-04-24T13:59:38Z |
| publishDate | 2019-06-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj.art-08421f2b8e0e4331b69e4cd51db246692024-04-03T12:48:49ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-06-0102414510.29326/2304-196X-2019-2-29-41-45407CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUSB. L. Manin0L. V. Malakhova1A. B. Sarbasov2N. V. Moroz3FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe paper presents experimental study results of the cytopathic effect of peste de petits ruminants virus on a goat gonad continuous cell line (YaDK-04). The interaction of peste de petits ruminants virus with cells at different stages of its reproduction was shown using a combination of phase-contrast and luminescent microscopy. It was found that at the initial stage of interaction (20–24 hours) the cells became rounded and de-adhered, and the monolayer was partially loosened. On day 2 post reproduction the most part of the culture monolayer affected by the virus began to destruct, and the cell nuclei were displaced to periphery. At the terminal stage (72 hours) the destruction of monolayer cells and cytoplasmic matrix, deformation and partial lysis of the nuclei and cytoplasm, aggregation of detritus occurred. At the final stage of reproduction (96 hours) the peste de petits ruminants virus diffused into the culture medium, the fluorescence in the yellow spectrum decreased significantly, but the virus titer reached 6.89 lg TCD50/cm3.https://veterinary.arriah.ru/jour/article/view/414yadc-04 cell culturepeste de petits ruminants viruscytopathic effectluminescent and phase-contrast microscopy |
| spellingShingle | B. L. Manin L. V. Malakhova A. B. Sarbasov N. V. Moroz CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS Ветеринария сегодня yadc-04 cell culture peste de petits ruminants virus cytopathic effect luminescent and phase-contrast microscopy |
| title | CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS |
| title_full | CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS |
| title_fullStr | CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS |
| title_full_unstemmed | CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS |
| title_short | CYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS |
| title_sort | cytomorphological transformations in yadk 04 cells during interaction with peste de petits ruminants virus |
| topic | yadc-04 cell culture peste de petits ruminants virus cytopathic effect luminescent and phase-contrast microscopy |
| url | https://veterinary.arriah.ru/jour/article/view/414 |
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