The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression
Recently, the possibility of cross-kingdom gene expression regulation by miRNAs from other species (“xenomiRs”), specifically from plants, has acquired scientific meaning. Based on the one of oldest methods for dealing with inflammation via the use of cabbage leaf compresses, we investigated the eff...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.846830/full |
| _version_ | 1811271641629458432 |
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| author | Kaja Kasarello Iwona Köhling Anna Kosowska Katarzyna Pucia Anna Lukasik Agnieszka Cudnoch-Jedrzejewska Leszek Paczek Leszek Paczek Urszula Zielenkiewicz Piotr Zielenkiewicz Piotr Zielenkiewicz |
| author_facet | Kaja Kasarello Iwona Köhling Anna Kosowska Katarzyna Pucia Anna Lukasik Agnieszka Cudnoch-Jedrzejewska Leszek Paczek Leszek Paczek Urszula Zielenkiewicz Piotr Zielenkiewicz Piotr Zielenkiewicz |
| author_sort | Kaja Kasarello |
| collection | DOAJ |
| description | Recently, the possibility of cross-kingdom gene expression regulation by miRNAs from other species (“xenomiRs”), specifically from plants, has acquired scientific meaning. Based on the one of oldest methods for dealing with inflammation via the use of cabbage leaf compresses, we investigated the effects of Brassica oleracea derived miR172a on the potential human target gene encoding FAN (Factor Associated with Neutral Sphingomyelinase Activation) protein. In vitro experiments showed a decrease in FAN protein levels in both human and mouse cells transfected with bol-miRNA172a. As the FAN protein mediates inflammatory responses, the potential of miR172a to mitigate the inflammatory process was tested in a mouse model of rheumatoid arthritis. Animal studies showed the decreased oedema of inflamed paws in mouse with rheumatoid arthritis model induced after treatment with miR172a. |
| first_indexed | 2024-04-12T22:24:41Z |
| format | Article |
| id | doaj.art-0842c2b67ce64b9ba005cd2e529b9abe |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T22:24:41Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-0842c2b67ce64b9ba005cd2e529b9abe2022-12-22T03:14:13ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-03-011310.3389/fphar.2022.846830846830The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN ExpressionKaja Kasarello0Iwona Köhling1Anna Kosowska2Katarzyna Pucia3Anna Lukasik4Agnieszka Cudnoch-Jedrzejewska5Leszek Paczek6Leszek Paczek7Urszula Zielenkiewicz8Piotr Zielenkiewicz9Piotr Zielenkiewicz10Centre for Preclinical Research, Department of Experimental and Clinical Physiology, Medical University of Warsaw, Warsaw, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Histology and Embryology, Medical University of Warsaw, Warsaw, PolandDepartment of Histology and Embryology, Medical University of Warsaw, Warsaw, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandCentre for Preclinical Research, Department of Experimental and Clinical Physiology, Medical University of Warsaw, Warsaw, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandDepartment of Immunology, Transplantology and Internal Diseases, Medical University of Warsaw, Warsaw, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, PolandFaculty of Biology, University of Warsaw, Warsaw, PolandRecently, the possibility of cross-kingdom gene expression regulation by miRNAs from other species (“xenomiRs”), specifically from plants, has acquired scientific meaning. Based on the one of oldest methods for dealing with inflammation via the use of cabbage leaf compresses, we investigated the effects of Brassica oleracea derived miR172a on the potential human target gene encoding FAN (Factor Associated with Neutral Sphingomyelinase Activation) protein. In vitro experiments showed a decrease in FAN protein levels in both human and mouse cells transfected with bol-miRNA172a. As the FAN protein mediates inflammatory responses, the potential of miR172a to mitigate the inflammatory process was tested in a mouse model of rheumatoid arthritis. Animal studies showed the decreased oedema of inflamed paws in mouse with rheumatoid arthritis model induced after treatment with miR172a.https://www.frontiersin.org/articles/10.3389/fphar.2022.846830/fullxenomiRsrheumatoid arthritisinflammationcollagen-induced arthritisFAN protein |
| spellingShingle | Kaja Kasarello Iwona Köhling Anna Kosowska Katarzyna Pucia Anna Lukasik Agnieszka Cudnoch-Jedrzejewska Leszek Paczek Leszek Paczek Urszula Zielenkiewicz Piotr Zielenkiewicz Piotr Zielenkiewicz The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression Frontiers in Pharmacology xenomiRs rheumatoid arthritis inflammation collagen-induced arthritis FAN protein |
| title | The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression |
| title_full | The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression |
| title_fullStr | The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression |
| title_full_unstemmed | The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression |
| title_short | The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression |
| title_sort | anti inflammatory effect of cabbage leaves explained by the influence of bol mirna172a on fan expression |
| topic | xenomiRs rheumatoid arthritis inflammation collagen-induced arthritis FAN protein |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.846830/full |
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