Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco
<i>Salicornia brachiata</i> is an extreme halophyte that commonly grows on marsh conditions and is also considered a promising resource for drought and salt-responsive genes. To unveil a glimpse of stress endurance by plants, it is of the utmost importance to develop an understanding of...
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MDPI AG
2021-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/1/62 |
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| author | Rajesh Kumar Jha Avinash Mishra |
| author_facet | Rajesh Kumar Jha Avinash Mishra |
| author_sort | Rajesh Kumar Jha |
| collection | DOAJ |
| description | <i>Salicornia brachiata</i> is an extreme halophyte that commonly grows on marsh conditions and is also considered a promising resource for drought and salt-responsive genes. To unveil a glimpse of stress endurance by plants, it is of the utmost importance to develop an understanding of stress tolerance mechanisms. ‘Early Responsive to Dehydration’ (ERD) genes are defined as a group of genes involved in stress tolerance and the development of plants. To increase this understanding, parallel to this expedited thought, a novel <i>SbERD4</i> gene was cloned from <i>S. brachiata</i>, characterized, and functionally validated in the model plant tobacco. The study showed that <i>SbERD4</i> is a plasma-membrane bound protein, and its overexpression in tobacco plants improved salinity and osmotic stress tolerance. Transgenic plants showed high relative water, chlorophylls, sugars, starch, polyphenols, proline, free amino acids, and low electrolyte leakage and H<sub>2</sub>O<sub>2</sub> content compared to control plants (wild type and vector control) under different abiotic stress conditions. Furthermore, the transcript expression of antioxidant enzyme encoding genes <i>NtCAT</i>, <i>NtSOD</i>, <i>NtGR</i>, and <i>NtAPX</i> showed higher expression in transgenic compared to wild-type and vector controls under varying stress conditions. Overall, the overexpression of a novel early responsive to dehydration stress protein 4-encoding gene (<i>SbERD4</i>) enhanced the tolerance of the plant against multiple abiotic stresses. In conclusion, the overexpression of the <i>SbERD4</i> gene mitigates plant physiology by enduring stress tolerance and might be considered as a promising key gene for engineering salinity and drought stress tolerance in crops. |
| first_indexed | 2024-03-10T03:46:01Z |
| format | Article |
| id | doaj.art-0843828a9b424707a7a48907affa7b23 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T03:46:01Z |
| publishDate | 2021-12-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-0843828a9b424707a7a48907affa7b232023-11-23T11:19:36ZengMDPI AGCells2073-44092021-12-011116210.3390/cells11010062Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic TobaccoRajesh Kumar Jha0Avinash Mishra1Division of Applied Phycology and Biotechnology, CSIR–Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, IndiaDivision of Applied Phycology and Biotechnology, CSIR–Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, India<i>Salicornia brachiata</i> is an extreme halophyte that commonly grows on marsh conditions and is also considered a promising resource for drought and salt-responsive genes. To unveil a glimpse of stress endurance by plants, it is of the utmost importance to develop an understanding of stress tolerance mechanisms. ‘Early Responsive to Dehydration’ (ERD) genes are defined as a group of genes involved in stress tolerance and the development of plants. To increase this understanding, parallel to this expedited thought, a novel <i>SbERD4</i> gene was cloned from <i>S. brachiata</i>, characterized, and functionally validated in the model plant tobacco. The study showed that <i>SbERD4</i> is a plasma-membrane bound protein, and its overexpression in tobacco plants improved salinity and osmotic stress tolerance. Transgenic plants showed high relative water, chlorophylls, sugars, starch, polyphenols, proline, free amino acids, and low electrolyte leakage and H<sub>2</sub>O<sub>2</sub> content compared to control plants (wild type and vector control) under different abiotic stress conditions. Furthermore, the transcript expression of antioxidant enzyme encoding genes <i>NtCAT</i>, <i>NtSOD</i>, <i>NtGR</i>, and <i>NtAPX</i> showed higher expression in transgenic compared to wild-type and vector controls under varying stress conditions. Overall, the overexpression of a novel early responsive to dehydration stress protein 4-encoding gene (<i>SbERD4</i>) enhanced the tolerance of the plant against multiple abiotic stresses. In conclusion, the overexpression of the <i>SbERD4</i> gene mitigates plant physiology by enduring stress tolerance and might be considered as a promising key gene for engineering salinity and drought stress tolerance in crops.https://www.mdpi.com/2073-4409/11/1/62abiotic stressdroughtgenetic transformationhalophytesalinitytransgenic |
| spellingShingle | Rajesh Kumar Jha Avinash Mishra Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco Cells abiotic stress drought genetic transformation halophyte salinity transgenic |
| title | Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco |
| title_full | Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco |
| title_fullStr | Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco |
| title_full_unstemmed | Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco |
| title_short | Introgression of <i>SbERD4</i> Gene Encodes an Early-Responsive Dehydration-Stress Protein That Confers Tolerance against Different Types of Abiotic Stresses in Transgenic Tobacco |
| title_sort | introgression of i sberd4 i gene encodes an early responsive dehydration stress protein that confers tolerance against different types of abiotic stresses in transgenic tobacco |
| topic | abiotic stress drought genetic transformation halophyte salinity transgenic |
| url | https://www.mdpi.com/2073-4409/11/1/62 |
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