Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients
Abstract COVID‐19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role of extracellular vesicles (EVs) in COVID‐19 and their biomarker potential, however, remains largely unknown. Aiming to identify specific EV signatures...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Journal of Extracellular Vesicles |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jev2.12257 |
| _version_ | 1798039423531614208 |
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| author | Tobias Tertel Sergej Tomić Jelena Đokić Dušan Radojević Dejan Stevanović Nataša Ilić Bernd Giebel Maja Kosanović |
| author_facet | Tobias Tertel Sergej Tomić Jelena Đokić Dušan Radojević Dejan Stevanović Nataša Ilić Bernd Giebel Maja Kosanović |
| author_sort | Tobias Tertel |
| collection | DOAJ |
| description | Abstract COVID‐19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role of extracellular vesicles (EVs) in COVID‐19 and their biomarker potential, however, remains largely unknown. Aiming to identify specific EV signatures of patients with mild compared to severe COVID‐19, we characterized the EV composition of 20 mild and 26 severe COVID‐19 patients along with 16 sex and age‐matched healthy donors with a panel of eight different antibodies by imaging flow cytometry (IFCM). We correlated the obtained data with 37 clinical, prerecorded biochemical and immunological parameters. Severe patients’ sera contained increased amounts of CD13+ and CD82+ EVs, which positively correlated with IL‐6‐producing and circulating myeloid‐derived suppressor cells (MDSCs) and with the serum concentration of proinflammatory cytokines, respectively. Sera of mild COVID‐19 patients contained more HLA‐ABC+ EVs than sera of the healthy donors and more CD24+ EVs than severe COVID‐19 patients. Their increased abundance negatively correlated with disease severity and accumulation of MDSCs, being considered as key drivers of immunopathogenesis in COVID‐19. Altogether, our results support the potential of serum EVs as powerful biomarkers for COVID‐19 severity and pave the way for future investigations aiming to unravel the role of EVs in COVID‐19 progression. |
| first_indexed | 2024-04-11T21:53:41Z |
| format | Article |
| id | doaj.art-0845bf63484d4f19b9d8bf1e36480c5e |
| institution | Directory Open Access Journal |
| issn | 2001-3078 |
| language | English |
| last_indexed | 2024-04-11T21:53:41Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Vesicles |
| spelling | doaj.art-0845bf63484d4f19b9d8bf1e36480c5e2022-12-22T04:01:10ZengWileyJournal of Extracellular Vesicles2001-30782022-08-01118n/an/a10.1002/jev2.12257Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patientsTobias Tertel0Sergej Tomić1Jelena Đokić2Dušan Radojević3Dejan Stevanović4Nataša Ilić5Bernd Giebel6Maja Kosanović7Institute for Transfusion Medicine University Hospital Essen, University of Duisburg‐Essen Essen GermanyInstitute for the Application of Nuclear Energy, INEP University of Belgrade Zemun‐Belgrade SerbiaInstitute of Molecular Genetics and Genetic Engineering (IMGGI) University of Belgrade Belgrade SerbiaInstitute of Molecular Genetics and Genetic Engineering (IMGGI) University of Belgrade Belgrade SerbiaClinical Hospital Center Zemun, Faculty of Medicine University of Belgrade Belgrade SerbiaInstitute for the Application of Nuclear Energy, INEP University of Belgrade Zemun‐Belgrade SerbiaInstitute for Transfusion Medicine University Hospital Essen, University of Duisburg‐Essen Essen GermanyInstitute for the Application of Nuclear Energy, INEP University of Belgrade Zemun‐Belgrade SerbiaAbstract COVID‐19 is characterized by a wide spectrum of disease severity, whose indicators and underlying mechanisms need to be identified. The role of extracellular vesicles (EVs) in COVID‐19 and their biomarker potential, however, remains largely unknown. Aiming to identify specific EV signatures of patients with mild compared to severe COVID‐19, we characterized the EV composition of 20 mild and 26 severe COVID‐19 patients along with 16 sex and age‐matched healthy donors with a panel of eight different antibodies by imaging flow cytometry (IFCM). We correlated the obtained data with 37 clinical, prerecorded biochemical and immunological parameters. Severe patients’ sera contained increased amounts of CD13+ and CD82+ EVs, which positively correlated with IL‐6‐producing and circulating myeloid‐derived suppressor cells (MDSCs) and with the serum concentration of proinflammatory cytokines, respectively. Sera of mild COVID‐19 patients contained more HLA‐ABC+ EVs than sera of the healthy donors and more CD24+ EVs than severe COVID‐19 patients. Their increased abundance negatively correlated with disease severity and accumulation of MDSCs, being considered as key drivers of immunopathogenesis in COVID‐19. Altogether, our results support the potential of serum EVs as powerful biomarkers for COVID‐19 severity and pave the way for future investigations aiming to unravel the role of EVs in COVID‐19 progression.https://doi.org/10.1002/jev2.12257biomarkersCOVID‐19extracellular vesiclesIFCMMDSCserum |
| spellingShingle | Tobias Tertel Sergej Tomić Jelena Đokić Dušan Radojević Dejan Stevanović Nataša Ilić Bernd Giebel Maja Kosanović Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients Journal of Extracellular Vesicles biomarkers COVID‐19 extracellular vesicles IFCM MDSC serum |
| title | Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients |
| title_full | Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients |
| title_fullStr | Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients |
| title_full_unstemmed | Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients |
| title_short | Serum‐derived extracellular vesicles: Novel biomarkers reflecting the disease severity of COVID‐19 patients |
| title_sort | serum derived extracellular vesicles novel biomarkers reflecting the disease severity of covid 19 patients |
| topic | biomarkers COVID‐19 extracellular vesicles IFCM MDSC serum |
| url | https://doi.org/10.1002/jev2.12257 |
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