Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels
Three-dimensional (3D) neuronal cultures are valuable models for studying brain complexity in vitro, and the choice of the bulk material in which the neurons grow is a crucial factor in establishing successful cultures. Indeed, neuronal development and network functionality are influenced by the mec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Gels |
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| Online Access: | https://www.mdpi.com/2310-2861/9/8/642 |
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| author | Clara F. López-León Jordi Soriano Ramon Planet |
| author_facet | Clara F. López-León Jordi Soriano Ramon Planet |
| author_sort | Clara F. López-León |
| collection | DOAJ |
| description | Three-dimensional (3D) neuronal cultures are valuable models for studying brain complexity in vitro, and the choice of the bulk material in which the neurons grow is a crucial factor in establishing successful cultures. Indeed, neuronal development and network functionality are influenced by the mechanical properties of the selected material; in turn, these properties may change due to neuron–matrix interactions that alter the microstructure of the material. To advance our understanding of the interplay between neurons and their environment, here we utilized a PEGylated fibrin hydrogel as a scaffold for mouse primary neuronal cultures and carried out a rheological characterization of the scaffold over a three-week period, both with and without cells. We observed that the hydrogels exhibited an elastic response that could be described in terms of the Young’s modulus <i>E</i>. The hydrogels without neurons procured a stable <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>E</mi><mo>≃</mo><mn>420</mn></mrow></semantics></math></inline-formula> Pa, while the neuron-laden hydrogels showed a higher <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>E</mi><mo>≃</mo><mn>590</mn></mrow></semantics></math></inline-formula> Pa during the early stages of development that decreased to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>E</mi><mo>≃</mo><mn>340</mn></mrow></semantics></math></inline-formula> Pa at maturer stages. Our results suggest that neurons and their processes dynamically modify the hydrogel structure during development, potentially compromising both the stability of the material and the functional traits of the developing neuronal network. |
| first_indexed | 2024-03-10T23:54:57Z |
| format | Article |
| id | doaj.art-08474894b4bd4786ab3722ea17d79a32 |
| institution | Directory Open Access Journal |
| issn | 2310-2861 |
| language | English |
| last_indexed | 2024-03-10T23:54:57Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gels |
| spelling | doaj.art-08474894b4bd4786ab3722ea17d79a322023-11-19T01:13:41ZengMDPI AGGels2310-28612023-08-019864210.3390/gels9080642Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin HydrogelsClara F. López-León0Jordi Soriano1Ramon Planet2Departament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, SpainDepartament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, SpainDepartament de Física de la Matèria Condensada, Universitat de Barcelona, E-08028 Barcelona, SpainThree-dimensional (3D) neuronal cultures are valuable models for studying brain complexity in vitro, and the choice of the bulk material in which the neurons grow is a crucial factor in establishing successful cultures. Indeed, neuronal development and network functionality are influenced by the mechanical properties of the selected material; in turn, these properties may change due to neuron–matrix interactions that alter the microstructure of the material. To advance our understanding of the interplay between neurons and their environment, here we utilized a PEGylated fibrin hydrogel as a scaffold for mouse primary neuronal cultures and carried out a rheological characterization of the scaffold over a three-week period, both with and without cells. We observed that the hydrogels exhibited an elastic response that could be described in terms of the Young’s modulus <i>E</i>. The hydrogels without neurons procured a stable <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>E</mi><mo>≃</mo><mn>420</mn></mrow></semantics></math></inline-formula> Pa, while the neuron-laden hydrogels showed a higher <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>E</mi><mo>≃</mo><mn>590</mn></mrow></semantics></math></inline-formula> Pa during the early stages of development that decreased to <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>E</mi><mo>≃</mo><mn>340</mn></mrow></semantics></math></inline-formula> Pa at maturer stages. Our results suggest that neurons and their processes dynamically modify the hydrogel structure during development, potentially compromising both the stability of the material and the functional traits of the developing neuronal network.https://www.mdpi.com/2310-2861/9/8/642biomaterialsneuronal culturesrheological characterizationhydrogels |
| spellingShingle | Clara F. López-León Jordi Soriano Ramon Planet Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels Gels biomaterials neuronal cultures rheological characterization hydrogels |
| title | Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels |
| title_full | Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels |
| title_fullStr | Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels |
| title_full_unstemmed | Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels |
| title_short | Rheological Characterization of Three-Dimensional Neuronal Cultures Embedded in PEGylated Fibrin Hydrogels |
| title_sort | rheological characterization of three dimensional neuronal cultures embedded in pegylated fibrin hydrogels |
| topic | biomaterials neuronal cultures rheological characterization hydrogels |
| url | https://www.mdpi.com/2310-2861/9/8/642 |
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