SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon
Identification of molecular markers revealing polymorphism among the parental lines are prerequisite for mapping QTLs and genes for desired traits. The genomic regions which contributes to the accumulation of grain iron and zinc in rice could greatly help in rice bio fortification programs. A BC4F1...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2020-09-01
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| Series: | Electronic Journal of Plant Breeding |
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| _version_ | 1811190162276745216 |
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| author | Gowthami Chandu, Krishnam Raju Addanki, Divya Balakrishnan, Satendra K Mangrauthia, P. Sudhakar, A. Krishna Satya and Sarla Neelamraju |
| author_facet | Gowthami Chandu, Krishnam Raju Addanki, Divya Balakrishnan, Satendra K Mangrauthia, P. Sudhakar, A. Krishna Satya and Sarla Neelamraju |
| author_sort | Gowthami Chandu, Krishnam Raju Addanki, Divya Balakrishnan, Satendra K Mangrauthia, P. Sudhakar, A. Krishna Satya and Sarla Neelamraju |
| collection | DOAJ |
| description | Identification of molecular markers revealing polymorphism among the parental lines are prerequisite for mapping QTLs and genes for desired traits. The genomic regions which contributes to the accumulation of grain iron and zinc in rice could greatly help in rice bio fortification programs. A BC4F10 mapping population was earlier developed from the cross between an elite fine-grain Oryza sativa indica cultivar, BPT5204 and a wild progenitor specie O. rufipogon WR119. A total of 800 randomly selected SSR markers distributed on all the 12 chromosomes of rice including 50 gene specific markers related to grain iron, zinc and yield traits were used to identify the polymorphic loci between the two genotypes. In all, 166 markers (20.75 %) showed distinct polymorphism. 149 SSR markers (19%) out of 750 SSRs and 17 out of 50 gene-specific markers (36%) were polymorphic. The 17 polymorphic gene-specific markers were related to gene families OsZIP, OsYSL, OsNRAMP, OsNAAT, OsFRO, OsFDH, OsGSTU and OsPDR which are involved in metal transport and homeostasis in rice. Among the markers reported to be significantly associated with QTLs for grain iron, zinc and yield related traits, RM517, RM81A, RM264, OsYSL-7, RM5460, RM3874 were polymorphic in this study. |
| first_indexed | 2024-04-11T14:45:38Z |
| format | Article |
| id | doaj.art-0848c390888b40b28d827d22ddb3a8c2 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-04-11T14:45:38Z |
| publishDate | 2020-09-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-0848c390888b40b28d827d22ddb3a8c22022-12-22T04:17:38ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2020-09-0111384184710.37992/2020.1103.138SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogonGowthami Chandu, Krishnam Raju Addanki, Divya Balakrishnan, Satendra K Mangrauthia, P. Sudhakar, A. Krishna Satya and Sarla NeelamrajuIdentification of molecular markers revealing polymorphism among the parental lines are prerequisite for mapping QTLs and genes for desired traits. The genomic regions which contributes to the accumulation of grain iron and zinc in rice could greatly help in rice bio fortification programs. A BC4F10 mapping population was earlier developed from the cross between an elite fine-grain Oryza sativa indica cultivar, BPT5204 and a wild progenitor specie O. rufipogon WR119. A total of 800 randomly selected SSR markers distributed on all the 12 chromosomes of rice including 50 gene specific markers related to grain iron, zinc and yield traits were used to identify the polymorphic loci between the two genotypes. In all, 166 markers (20.75 %) showed distinct polymorphism. 149 SSR markers (19%) out of 750 SSRs and 17 out of 50 gene-specific markers (36%) were polymorphic. The 17 polymorphic gene-specific markers were related to gene families OsZIP, OsYSL, OsNRAMP, OsNAAT, OsFRO, OsFDH, OsGSTU and OsPDR which are involved in metal transport and homeostasis in rice. Among the markers reported to be significantly associated with QTLs for grain iron, zinc and yield related traits, RM517, RM81A, RM264, OsYSL-7, RM5460, RM3874 were polymorphic in this study.bpt5204wild ricebio fortificationssr markerspolymorphismironzinc |
| spellingShingle | Gowthami Chandu, Krishnam Raju Addanki, Divya Balakrishnan, Satendra K Mangrauthia, P. Sudhakar, A. Krishna Satya and Sarla Neelamraju SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon Electronic Journal of Plant Breeding bpt5204 wild rice bio fortification ssr markers polymorphism iron zinc |
| title | SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon |
| title_full | SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon |
| title_fullStr | SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon |
| title_full_unstemmed | SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon |
| title_short | SSR markers for grain iron zinc and yield-related traits polymorphic between Samba Mahsuri (BPT5204) and a wild rice Oryza rufipogon |
| title_sort | ssr markers for grain iron zinc and yield related traits polymorphic between samba mahsuri bpt5204 and a wild rice oryza rufipogon |
| topic | bpt5204 wild rice bio fortification ssr markers polymorphism iron zinc |
| work_keys_str_mv | AT gowthamichandukrishnamrajuaddankidivyabalakrishnansatendrakmangrauthiapsudhakarakrishnasatyaandsarlaneelamraju ssrmarkersforgrainironzincandyieldrelatedtraitspolymorphicbetweensambamahsuribpt5204andawildriceoryzarufipogon |