Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot
Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonucle...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2002-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013 |
| _version_ | 1811207335761149952 |
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| author | Santos Carlos A.F. Simon Philipp W. |
| author_facet | Santos Carlos A.F. Simon Philipp W. |
| author_sort | Santos Carlos A.F. |
| collection | DOAJ |
| description | Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six conserved AFLPs only one mapped to different linkage groups in the two populations while four of the five less-conserved bands mapped to different linkage groups. Of eight SCAR (sequence characterized amplified regions) primers tested, one conserved AFLP resulted in co-dominant markers in both populations. Screening among 14 carrot inbreds or cultivars with three AFLP-SCAR primers revealed clear and polymorphic PCR products, with similar molecular sizes on agarose gels. The development of co-dominant markers based on conserved AFLP fragments will be useful to detect seed mixtures among hybrids, to improve and to merge linkage maps and to study diversity and phylogenetic relationships. |
| first_indexed | 2024-04-12T04:01:19Z |
| format | Article |
| id | doaj.art-084931fbd93341d3b6c9dfba4d8dd20d |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-04-12T04:01:19Z |
| publishDate | 2002-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-084931fbd93341d3b6c9dfba4d8dd20d2022-12-22T03:48:42ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852002-01-01252195201Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrotSantos Carlos A.F.Simon Philipp W.Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six conserved AFLPs only one mapped to different linkage groups in the two populations while four of the five less-conserved bands mapped to different linkage groups. Of eight SCAR (sequence characterized amplified regions) primers tested, one conserved AFLP resulted in co-dominant markers in both populations. Screening among 14 carrot inbreds or cultivars with three AFLP-SCAR primers revealed clear and polymorphic PCR products, with similar molecular sizes on agarose gels. The development of co-dominant markers based on conserved AFLP fragments will be useful to detect seed mixtures among hybrids, to improve and to merge linkage maps and to study diversity and phylogenetic relationships.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013Daucus carotaevolutionmolecular markersSCAR |
| spellingShingle | Santos Carlos A.F. Simon Philipp W. Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot Genetics and Molecular Biology Daucus carota evolution molecular markers SCAR |
| title | Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot |
| title_full | Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot |
| title_fullStr | Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot |
| title_full_unstemmed | Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot |
| title_short | Some AFLP amplicons are highly conserved DNA sequences mapping to the same linkage groups in two F2 populations of carrot |
| title_sort | some aflp amplicons are highly conserved dna sequences mapping to the same linkage groups in two f2 populations of carrot |
| topic | Daucus carota evolution molecular markers SCAR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013 |
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