Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the <i>Gardnerella</i>-Specific Endolysin PM-477, Directly in Vaginal Samples from Women with Bacterial Vaginosis

Optimization of Propidium Monoazide qPCR (Viability-qPCR) to Quantify the Killing by the <i>Gardnerella</i>-Specific Endolysin PM-477, Directly in Vaginal Samples from Women with Bacterial Vaginosis

Quantification of the number of living cells in biofilm or after eradication treatments of biofilm, is problematic for different reasons. We assessed the performance of pre-treatment of DNA, planktonic cells and ex vivo vaginal biofilms of <i>Gardnerella</i> with propidium monoazide (PMA...

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Bibliographic Details
Main Authors: Agnieszka Latka, Leen Van Simaey, Marijke Reynders, Piet Cools, Tess Rogier, Barbara Lebbe, Lorenzo Corsini, Christine Landlinger, Mario Vaneechoutte
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Antibiotics
Subjects:
endolysin
bacterial vaginosis
biofilm
<i>Gardnerella</i>
viability-qPCR
Online Access:https://www.mdpi.com/2079-6382/11/1/111

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