Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions
Microencapsulation is a promising method that has considerable effects on protection of probiotic viability. A variety of coating materials have been utilized to enhance the stability of probiotic microorganisms during the transition through gastrointestinal tract. The aim of this research was to de...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2019-02-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2483 |
| _version_ | 1797800613403164672 |
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| author | Emel UNAL TURHAN |
| author_facet | Emel UNAL TURHAN |
| author_sort | Emel UNAL TURHAN |
| collection | DOAJ |
| description | Microencapsulation is a promising method that has considerable effects on protection of probiotic viability. A variety of coating materials
have been utilized to enhance the stability of probiotic microorganisms during the transition through gastrointestinal tract. The aim of
this research was to determine optimum coating material combinations for probiotic microencapsulation against gastric conditions.
Fructooligosaccharides, peptide, sodium alginate, gelatin and gellan gum were used as entrapment substances to microencapsulate
Lactobacillus plantarum and Lactobacillus casei Shirota with extrusion technique. The response surface technique was applied to detect
the optimum proportion of encapsulation substances against gastric condition. Microencapsulation protected probiotic cultures against
stress factors such as simulated gastric juice and bile-salt solution. Optimum rate of encapsulation substances varied according to the type
of probiotic bacteria. Test results showed that L. plantarum should be coated with 1.5% alginate, 0.92% gellan gum, 0.18% gelatin, 0.36%
peptide and 1.31% fructooligosaccharides for highest protection. L. casei Shirota should also be coated with 2% alginate, 0.98% gellan gum,
0.51% gelatin, 0.86% peptide and 1.98% fructooligosaccharides for highest protection. This research concluded that microencapsulation
with encapsulation materials at optimum concentration provided improved protection for the probiotics. |
| first_indexed | 2024-03-13T04:37:54Z |
| format | Article |
| id | doaj.art-084b86eb38a7447793d9cc8a792bb0dd |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:54Z |
| publishDate | 2019-02-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-084b86eb38a7447793d9cc8a792bb0dd2023-06-19T06:34:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-02-0125453153710.9775/kvfd.2018.212742483Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditionsEmel UNAL TURHAN0Osmaniye Korkut Ata University, Kadirli Applied Sciences School, Department of Food Technology, TR-80760 Osmaniye - TURKEYMicroencapsulation is a promising method that has considerable effects on protection of probiotic viability. A variety of coating materials have been utilized to enhance the stability of probiotic microorganisms during the transition through gastrointestinal tract. The aim of this research was to determine optimum coating material combinations for probiotic microencapsulation against gastric conditions. Fructooligosaccharides, peptide, sodium alginate, gelatin and gellan gum were used as entrapment substances to microencapsulate Lactobacillus plantarum and Lactobacillus casei Shirota with extrusion technique. The response surface technique was applied to detect the optimum proportion of encapsulation substances against gastric condition. Microencapsulation protected probiotic cultures against stress factors such as simulated gastric juice and bile-salt solution. Optimum rate of encapsulation substances varied according to the type of probiotic bacteria. Test results showed that L. plantarum should be coated with 1.5% alginate, 0.92% gellan gum, 0.18% gelatin, 0.36% peptide and 1.31% fructooligosaccharides for highest protection. L. casei Shirota should also be coated with 2% alginate, 0.98% gellan gum, 0.51% gelatin, 0.86% peptide and 1.98% fructooligosaccharides for highest protection. This research concluded that microencapsulation with encapsulation materials at optimum concentration provided improved protection for the probiotics.https://vetdergikafkas.org/pdf.php?id=2483lactobacillus casei shirotalactobacillus plantarummicroencapsulationresponse surface methodextrusion |
| spellingShingle | Emel UNAL TURHAN Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions Kafkas Universitesi Veteriner Fakültesi Dergisi lactobacillus casei shirota lactobacillus plantarum microencapsulation response surface method extrusion |
| title | Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions |
| title_full | Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions |
| title_fullStr | Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions |
| title_full_unstemmed | Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions |
| title_short | Optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions |
| title_sort | optimization of entrapment substances for microencapsulation of lactobacillus plantarum and lactobacillus casei shirota against gastric conditions |
| topic | lactobacillus casei shirota lactobacillus plantarum microencapsulation response surface method extrusion |
| url | https://vetdergikafkas.org/pdf.php?id=2483 |
| work_keys_str_mv | AT emelunalturhan optimizationofentrapmentsubstancesformicroencapsulationoflactobacillusplantarumandlactobacilluscaseishirotaagainstgastricconditions |