Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface
It has been demonstrated that geometry can affect cell behaviors. Though curvature-sensitive proteins at the nanoscale are studied, it is unclear how cells sense curvature at the cellular and multicellular levels. To characterize and determine the mechanisms of curvature-dependent cell behaviors, we...
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| Format: | Article |
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MDPI AG
2019-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/8/813 |
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| author | Wei-Hung Jung Khalid Elawad Sung Hoon Kang Yun Chen |
| author_facet | Wei-Hung Jung Khalid Elawad Sung Hoon Kang Yun Chen |
| author_sort | Wei-Hung Jung |
| collection | DOAJ |
| description | It has been demonstrated that geometry can affect cell behaviors. Though curvature-sensitive proteins at the nanoscale are studied, it is unclear how cells sense curvature at the cellular and multicellular levels. To characterize and determine the mechanisms of curvature-dependent cell behaviors, we grow cells on open channels of the 60-µm radius. We found that cortical F-actin is 1.2-fold more enriched in epithelial cells grown on the curved surface compared to the flat control. We observed that myosin activity is required to promote cortical F-actin formation. Furthermore, cell−cell contact was shown to be indispensable for curvature-dependent cortical actin assembly. Our results indicate that the actomyosin network coupled with adherens junctions is involved in curvature-sensing at the multi-cellular level. |
| first_indexed | 2024-03-12T06:07:19Z |
| format | Article |
| id | doaj.art-084c1070fc1c47e998639e2051b356c0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T06:07:19Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-084c1070fc1c47e998639e2051b356c02023-09-03T03:25:34ZengMDPI AGCells2073-44092019-08-018881310.3390/cells8080813cells8080813Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved SurfaceWei-Hung Jung0Khalid Elawad1Sung Hoon Kang2Yun Chen3Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USADepartment of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USADepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USADepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218, USAIt has been demonstrated that geometry can affect cell behaviors. Though curvature-sensitive proteins at the nanoscale are studied, it is unclear how cells sense curvature at the cellular and multicellular levels. To characterize and determine the mechanisms of curvature-dependent cell behaviors, we grow cells on open channels of the 60-µm radius. We found that cortical F-actin is 1.2-fold more enriched in epithelial cells grown on the curved surface compared to the flat control. We observed that myosin activity is required to promote cortical F-actin formation. Furthermore, cell−cell contact was shown to be indispensable for curvature-dependent cortical actin assembly. Our results indicate that the actomyosin network coupled with adherens junctions is involved in curvature-sensing at the multi-cellular level.https://www.mdpi.com/2073-4409/8/8/813curvature sensingadherens junctionE-cadherincortical actinmyosin |
| spellingShingle | Wei-Hung Jung Khalid Elawad Sung Hoon Kang Yun Chen Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface Cells curvature sensing adherens junction E-cadherin cortical actin myosin |
| title | Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface |
| title_full | Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface |
| title_fullStr | Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface |
| title_full_unstemmed | Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface |
| title_short | Cell–Cell Adhesion and Myosin Activity Regulate Cortical Actin Assembly in Mammary Gland Epithelium on Concaved Surface |
| title_sort | cell cell adhesion and myosin activity regulate cortical actin assembly in mammary gland epithelium on concaved surface |
| topic | curvature sensing adherens junction E-cadherin cortical actin myosin |
| url | https://www.mdpi.com/2073-4409/8/8/813 |
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