Locational memory of macrovessel vascular cells is transcriptionally imprinted
Abstract Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-38880-6 |
| _version_ | 1797452491216912384 |
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| author | Talitha C. F. Spanjersberg Loes A. Oosterhoff Hedwig S. Kruitwagen Noortje A. M. van den Dungen Johannes C. M. Vernooij Folkert W. Asselbergs Michal Mokry Bart Spee Magdalena Harakalova Frank G. van Steenbeek |
| author_facet | Talitha C. F. Spanjersberg Loes A. Oosterhoff Hedwig S. Kruitwagen Noortje A. M. van den Dungen Johannes C. M. Vernooij Folkert W. Asselbergs Michal Mokry Bart Spee Magdalena Harakalova Frank G. van Steenbeek |
| author_sort | Talitha C. F. Spanjersberg |
| collection | DOAJ |
| description | Abstract Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We observed that organ-specific expression patterns persist in vitro, indicating that these genes are not regulated by blood flow or surrounding cell types but are likely fixed in the epigenetic memory. We further demonstrated the preserved location-specific expression of GATA4 protein in cultured cells and in the primary adult vessel. On a functional level, arterial and venous endothelial cells differed in vascular network morphology as the arterial networks maintained a higher complexity. Our findings prompt the rethinking of the extrapolation of results from single-origin endothelial cell systems. |
| first_indexed | 2024-03-09T15:09:26Z |
| format | Article |
| id | doaj.art-084d9af137ee4c1b8e300e79be3a0038 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:09:26Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-084d9af137ee4c1b8e300e79be3a00382023-11-26T13:25:28ZengNature PortfolioScientific Reports2045-23222023-08-0113111410.1038/s41598-023-38880-6Locational memory of macrovessel vascular cells is transcriptionally imprintedTalitha C. F. Spanjersberg0Loes A. Oosterhoff1Hedwig S. Kruitwagen2Noortje A. M. van den Dungen3Johannes C. M. Vernooij4Folkert W. Asselbergs5Michal Mokry6Bart Spee7Magdalena Harakalova8Frank G. van Steenbeek9Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht UniversityRegenerative Medicine Centre Utrecht, University Medical Center Utrecht, Utrecht UniversityDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht UniversityCentral Diagnostics Laboratory, University Medical Center Utrecht, Utrecht UniversityDepartment of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht UniversityDepartment of Cardiology, Amsterdam University Medical Centers, University of AmsterdamCentral Diagnostics Laboratory, University Medical Center Utrecht, Utrecht UniversityDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht UniversityRegenerative Medicine Centre Utrecht, University Medical Center Utrecht, Utrecht UniversityDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht UniversityAbstract Vascular pathologies show locational predisposition throughout the body; further insights into the transcriptomics basis of this vascular heterogeneity are needed. We analyzed transcriptomes from cultured endothelial cells and vascular smooth muscle cells from nine adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We observed that organ-specific expression patterns persist in vitro, indicating that these genes are not regulated by blood flow or surrounding cell types but are likely fixed in the epigenetic memory. We further demonstrated the preserved location-specific expression of GATA4 protein in cultured cells and in the primary adult vessel. On a functional level, arterial and venous endothelial cells differed in vascular network morphology as the arterial networks maintained a higher complexity. Our findings prompt the rethinking of the extrapolation of results from single-origin endothelial cell systems.https://doi.org/10.1038/s41598-023-38880-6 |
| spellingShingle | Talitha C. F. Spanjersberg Loes A. Oosterhoff Hedwig S. Kruitwagen Noortje A. M. van den Dungen Johannes C. M. Vernooij Folkert W. Asselbergs Michal Mokry Bart Spee Magdalena Harakalova Frank G. van Steenbeek Locational memory of macrovessel vascular cells is transcriptionally imprinted Scientific Reports |
| title | Locational memory of macrovessel vascular cells is transcriptionally imprinted |
| title_full | Locational memory of macrovessel vascular cells is transcriptionally imprinted |
| title_fullStr | Locational memory of macrovessel vascular cells is transcriptionally imprinted |
| title_full_unstemmed | Locational memory of macrovessel vascular cells is transcriptionally imprinted |
| title_short | Locational memory of macrovessel vascular cells is transcriptionally imprinted |
| title_sort | locational memory of macrovessel vascular cells is transcriptionally imprinted |
| url | https://doi.org/10.1038/s41598-023-38880-6 |
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