Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol
Ambient air pollution is one of the leading five health risks worldwide. One of the most harmful air pollutants is particulate matter (PM), which has different physical characteristics (particle size and number, surface area and morphology) and a highly complex and variable chemical composition. Our...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673561/?tool=EBI |
| _version_ | 1818116340765949952 |
|---|---|
| author | Zaira Leni Laure Estelle Cassagnes Kaspar R. Daellenbach Imad El Haddad Athanasia Vlachou Gaelle Uzu André S. H. Prévôt Jean-Luc Jaffrezo Nathalie Baumlin Matthias Salathe Urs Baltensperger Josef Dommen Marianne Geiser Rodrigo Franco |
| author_facet | Zaira Leni Laure Estelle Cassagnes Kaspar R. Daellenbach Imad El Haddad Athanasia Vlachou Gaelle Uzu André S. H. Prévôt Jean-Luc Jaffrezo Nathalie Baumlin Matthias Salathe Urs Baltensperger Josef Dommen Marianne Geiser Rodrigo Franco |
| author_sort | Zaira Leni |
| collection | DOAJ |
| description | Ambient air pollution is one of the leading five health risks worldwide. One of the most harmful air pollutants is particulate matter (PM), which has different physical characteristics (particle size and number, surface area and morphology) and a highly complex and variable chemical composition. Our goal was first to comparatively assess the effects of exposure to PM regarding cytotoxicity, release of pro-inflammatory mediators and gene expression in human bronchial epithelia (HBE) reflecting normal and compromised health status. Second, we aimed at evaluating the impact of various PM components from anthropogenic and biogenic sources on the cellular responses. Air-liquid interface (ALI) cultures of fully differentiated HBE derived from normal and cystic fibrosis (CF) donor lungs were exposed at the apical cell surface to water-soluble PM filter extracts for 4 h. The particle dose deposited on cells was 0.9–2.5 and 8.8–25.4 μg per cm2 of cell culture area for low and high PM doses, respectively. Both normal and CF HBE show a clear dose-response relationship with increasing cytotoxicity at higher PM concentrations. The concurrently enhanced release of pro-inflammatory mediators at higher PM exposure levels links cytotoxicity to inflammatory processes. Further, the PM exposure deregulates genes involved in oxidative stress and inflammatory pathways leading to an imbalance of the antioxidant system. Moreover, we identify compromised defense against PM in CF epithelia promoting exacerbation and aggravation of disease. We also demonstrate that the adverse health outcome induced by PM exposure in normal and particularly in susceptible bronchial epithelia is magnified by anthropogenic PM components. Thus, including health-relevant PM components in regulatory guidelines will result in substantial human health benefits and improve protection of the vulnerable population. |
| first_indexed | 2024-12-11T04:20:58Z |
| format | Article |
| id | doaj.art-084f8f19369942f59e09e0d5d2d0d7f6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T04:20:58Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-084f8f19369942f59e09e0d5d2d0d7f62022-12-22T01:21:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011511Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosolZaira LeniLaure Estelle CassagnesKaspar R. DaellenbachImad El HaddadAthanasia VlachouGaelle UzuAndré S. H. PrévôtJean-Luc JaffrezoNathalie BaumlinMatthias SalatheUrs BaltenspergerJosef DommenMarianne GeiserRodrigo FrancoAmbient air pollution is one of the leading five health risks worldwide. One of the most harmful air pollutants is particulate matter (PM), which has different physical characteristics (particle size and number, surface area and morphology) and a highly complex and variable chemical composition. Our goal was first to comparatively assess the effects of exposure to PM regarding cytotoxicity, release of pro-inflammatory mediators and gene expression in human bronchial epithelia (HBE) reflecting normal and compromised health status. Second, we aimed at evaluating the impact of various PM components from anthropogenic and biogenic sources on the cellular responses. Air-liquid interface (ALI) cultures of fully differentiated HBE derived from normal and cystic fibrosis (CF) donor lungs were exposed at the apical cell surface to water-soluble PM filter extracts for 4 h. The particle dose deposited on cells was 0.9–2.5 and 8.8–25.4 μg per cm2 of cell culture area for low and high PM doses, respectively. Both normal and CF HBE show a clear dose-response relationship with increasing cytotoxicity at higher PM concentrations. The concurrently enhanced release of pro-inflammatory mediators at higher PM exposure levels links cytotoxicity to inflammatory processes. Further, the PM exposure deregulates genes involved in oxidative stress and inflammatory pathways leading to an imbalance of the antioxidant system. Moreover, we identify compromised defense against PM in CF epithelia promoting exacerbation and aggravation of disease. We also demonstrate that the adverse health outcome induced by PM exposure in normal and particularly in susceptible bronchial epithelia is magnified by anthropogenic PM components. Thus, including health-relevant PM components in regulatory guidelines will result in substantial human health benefits and improve protection of the vulnerable population.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673561/?tool=EBI |
| spellingShingle | Zaira Leni Laure Estelle Cassagnes Kaspar R. Daellenbach Imad El Haddad Athanasia Vlachou Gaelle Uzu André S. H. Prévôt Jean-Luc Jaffrezo Nathalie Baumlin Matthias Salathe Urs Baltensperger Josef Dommen Marianne Geiser Rodrigo Franco Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol PLoS ONE |
| title | Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol |
| title_full | Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol |
| title_fullStr | Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol |
| title_full_unstemmed | Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol |
| title_short | Oxidative stress-induced inflammation in susceptible airways by anthropogenic aerosol |
| title_sort | oxidative stress induced inflammation in susceptible airways by anthropogenic aerosol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7673561/?tool=EBI |
| work_keys_str_mv | AT zairaleni oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT laureestellecassagnes oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT kasparrdaellenbach oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT imadelhaddad oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT athanasiavlachou oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT gaelleuzu oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT andreshprevot oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT jeanlucjaffrezo oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT nathaliebaumlin oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT matthiassalathe oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT ursbaltensperger oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT josefdommen oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT mariannegeiser oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol AT rodrigofranco oxidativestressinducedinflammationinsusceptibleairwaysbyanthropogenicaerosol |