The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque
Dextranase, a hydrolase that specifically hydrolyzes α-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of <i>Catenovulum agarivorans</i> MNH15 was screened from marine samples. When the temperature, initial...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/10/592 |
| _version_ | 1811262335947374592 |
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| author | Xiaohua Lai Xin Liu Xueqin Liu Tian Deng Yanli Feng Xiaopeng Tian Mingsheng Lyu Shujun Wang |
| author_facet | Xiaohua Lai Xin Liu Xueqin Liu Tian Deng Yanli Feng Xiaopeng Tian Mingsheng Lyu Shujun Wang |
| author_sort | Xiaohua Lai |
| collection | DOAJ |
| description | Dextranase, a hydrolase that specifically hydrolyzes α-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of <i>Catenovulum agarivorans</i> MNH15 was screened from marine samples. When the temperature, initial pH, NaCl concentration, and inducer concentration were 30 °C, 8.0, 5 g/L, and 8 g/L, respectively, it yielded more dextranase. The molecular weight of the dextranase was approximately 110 kDa. The maximum enzyme activity was achieved at 40 °C and a pH of 8.0. The enzyme was stable at 30 °C and a pH of 5−9. The metal ion Sr<sup>2+</sup> enhanced its activity, whereas NH<sup>4+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, and Li<sup>+</sup> had the opposite effect. The dextranase effectively inhibited the formation of biofilm by <i>Streptococcus mutans</i>. Moreover, sodium fluoride, xylitol, and sodium benzoate, all used in dental care products, had no significant effect on dextranase activity. In addition, high-performance liquid chromatography (HPLC) showed that dextran was mainly hydrolyzed to glucose, maltose, and maltoheptaose. The results indicated that dextranase has high application potential in dental products such as toothpaste and mouthwash. |
| first_indexed | 2024-04-12T19:23:29Z |
| format | Article |
| id | doaj.art-0854410a53e04f09b08369beee9ae0dd |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-12T19:23:29Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-0854410a53e04f09b08369beee9ae0dd2022-12-22T03:19:32ZengMDPI AGMarine Drugs1660-33972019-10-01171059210.3390/md17100592md17100592The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental PlaqueXiaohua Lai0Xin Liu1Xueqin Liu2Tian Deng3Yanli Feng4Xiaopeng Tian5Mingsheng Lyu6Shujun Wang7Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaJiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, ChinaDextranase, a hydrolase that specifically hydrolyzes α-1,6-glucosidic bonds, has been used in the pharmaceutical, food, and biotechnology industries. In this study, the strain of <i>Catenovulum agarivorans</i> MNH15 was screened from marine samples. When the temperature, initial pH, NaCl concentration, and inducer concentration were 30 °C, 8.0, 5 g/L, and 8 g/L, respectively, it yielded more dextranase. The molecular weight of the dextranase was approximately 110 kDa. The maximum enzyme activity was achieved at 40 °C and a pH of 8.0. The enzyme was stable at 30 °C and a pH of 5−9. The metal ion Sr<sup>2+</sup> enhanced its activity, whereas NH<sup>4+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, and Li<sup>+</sup> had the opposite effect. The dextranase effectively inhibited the formation of biofilm by <i>Streptococcus mutans</i>. Moreover, sodium fluoride, xylitol, and sodium benzoate, all used in dental care products, had no significant effect on dextranase activity. In addition, high-performance liquid chromatography (HPLC) showed that dextran was mainly hydrolyzed to glucose, maltose, and maltoheptaose. The results indicated that dextranase has high application potential in dental products such as toothpaste and mouthwash.https://www.mdpi.com/1660-3397/17/10/592<i>catenovulum agarivorans</i>dextranasedental plaqueoligo-saccharides |
| spellingShingle | Xiaohua Lai Xin Liu Xueqin Liu Tian Deng Yanli Feng Xiaopeng Tian Mingsheng Lyu Shujun Wang The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque Marine Drugs <i>catenovulum agarivorans</i> dextranase dental plaque oligo-saccharides |
| title | The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque |
| title_full | The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque |
| title_fullStr | The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque |
| title_full_unstemmed | The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque |
| title_short | The Marine <i>Catenovulum agarivorans</i> MNH15 and Dextranase: Removing Dental Plaque |
| title_sort | marine i catenovulum agarivorans i mnh15 and dextranase removing dental plaque |
| topic | <i>catenovulum agarivorans</i> dextranase dental plaque oligo-saccharides |
| url | https://www.mdpi.com/1660-3397/17/10/592 |
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