Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells
In this study, copper oxide nanoparticles (CuO NPs) were green synthesized using the leaf extract of Momordica cochinchinensis (Lour.). Various characterization techniques such as Energy-dispersive x-ray spectroscopy (EDS), Ultraviolet Visible (UV–vis) Spectroscopy, x-ray diffraction (XRD), Transmis...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2021-01-01
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| Series: | Materials Research Express |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/2053-1591/abd666 |
| _version_ | 1797746782166319104 |
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| author | Yu Liu Zheng Zeng Ou Jiang YiXian Li Qiang Xu LiJun Jiang Jing Yu Dexiao Xu |
| author_facet | Yu Liu Zheng Zeng Ou Jiang YiXian Li Qiang Xu LiJun Jiang Jing Yu Dexiao Xu |
| author_sort | Yu Liu |
| collection | DOAJ |
| description | In this study, copper oxide nanoparticles (CuO NPs) were green synthesized using the leaf extract of Momordica cochinchinensis (Lour.). Various characterization techniques such as Energy-dispersive x-ray spectroscopy (EDS), Ultraviolet Visible (UV–vis) Spectroscopy, x-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Fourier-transform Infrared (FTIR) Spectroscopy were utilized to study the formation of CuO NPs. DLS and TEM analysis revealed the formation of sphere-shaped CuO NPs with mean particle size of 56 nm. Additionally, the prepared CuO NPs were incubated with Hepatic (HepG2) cells to check their cel viability and evaluate the formation of reactive oxygen species (ROS). The results of the current work exhibited a concentration-dependent decline in the viability of HepG2 cells with half maximal inhibitory concentration (IC _50 ) value of 75 μ g ml ^−1 . The cytotoxic effect of CuO NPs was responsible for the cell apoptosis as well as ROS induction in the HepG2 cells. Further, the prepared CuO NPs could act as possible chemotherapeutic agent for the treatment of liver hepatocellular carcinoma (HCC) in future. |
| first_indexed | 2024-03-12T15:42:44Z |
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| id | doaj.art-0856b99a21664a83822c74d97a3a3ef8 |
| institution | Directory Open Access Journal |
| issn | 2053-1591 |
| language | English |
| last_indexed | 2024-03-12T15:42:44Z |
| publishDate | 2021-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj.art-0856b99a21664a83822c74d97a3a3ef82023-08-09T15:54:02ZengIOP PublishingMaterials Research Express2053-15912021-01-018101501110.1088/2053-1591/abd666Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cellsYu Liu0Zheng Zeng1Ou Jiang2YiXian Li3Qiang Xu4LiJun Jiang5Jing Yu6Dexiao Xu7https://orcid.org/0000-0002-4933-4998Department of Surgical Oncology, the Second people’s hospital of Neijiang, Neijiang 641000, Sichuan, People’s Republic of ChinaDepartment of Oncology, the Affiliated Hospital of Southwest Medical University , Luzhou 646000, Sichuan, People’s Republic of ChinaDepartment of Surgical Oncology, the Second people’s hospital of Neijiang, Neijiang 641000, Sichuan, People’s Republic of China; Department of Oncology, the Affiliated Hospital of Southwest Medical University , Luzhou 646000, Sichuan, People’s Republic of ChinaDepartment of Oncology, the Affiliated Hospital of Southwest Medical University , Luzhou 646000, Sichuan, People’s Republic of ChinaDepartment of Surgical Oncology, the Second people’s hospital of Neijiang, Neijiang 641000, Sichuan, People’s Republic of ChinaDepartment of Surgical Oncology, the Second people’s hospital of Neijiang, Neijiang 641000, Sichuan, People’s Republic of ChinaDepartment of Surgical Oncology, the Second people’s hospital of Neijiang, Neijiang 641000, Sichuan, People’s Republic of ChinaThe Second Department of Internal Medicine, Linyi Cancer Hospital, Linyi 276001, Shandong Province, People’s Republic of ChinaIn this study, copper oxide nanoparticles (CuO NPs) were green synthesized using the leaf extract of Momordica cochinchinensis (Lour.). Various characterization techniques such as Energy-dispersive x-ray spectroscopy (EDS), Ultraviolet Visible (UV–vis) Spectroscopy, x-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Fourier-transform Infrared (FTIR) Spectroscopy were utilized to study the formation of CuO NPs. DLS and TEM analysis revealed the formation of sphere-shaped CuO NPs with mean particle size of 56 nm. Additionally, the prepared CuO NPs were incubated with Hepatic (HepG2) cells to check their cel viability and evaluate the formation of reactive oxygen species (ROS). The results of the current work exhibited a concentration-dependent decline in the viability of HepG2 cells with half maximal inhibitory concentration (IC _50 ) value of 75 μ g ml ^−1 . The cytotoxic effect of CuO NPs was responsible for the cell apoptosis as well as ROS induction in the HepG2 cells. Further, the prepared CuO NPs could act as possible chemotherapeutic agent for the treatment of liver hepatocellular carcinoma (HCC) in future.https://doi.org/10.1088/2053-1591/abd666M. cochinchinensiscopper oxide nanoparticlesHepG2 cell linesin-vitro cytotoxicity. |
| spellingShingle | Yu Liu Zheng Zeng Ou Jiang YiXian Li Qiang Xu LiJun Jiang Jing Yu Dexiao Xu Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells Materials Research Express M. cochinchinensis copper oxide nanoparticles HepG2 cell lines in-vitro cytotoxicity. |
| title | Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells |
| title_full | Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells |
| title_fullStr | Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells |
| title_full_unstemmed | Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells |
| title_short | Green synthesis of CuO NPs, characterization and their toxicity potential against HepG2 cells |
| title_sort | green synthesis of cuo nps characterization and their toxicity potential against hepg2 cells |
| topic | M. cochinchinensis copper oxide nanoparticles HepG2 cell lines in-vitro cytotoxicity. |
| url | https://doi.org/10.1088/2053-1591/abd666 |
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