Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression
This study aimed to observe the anticancer, as well as radiosensitizing, effect of Anagallis arvensis extract (AAE) on the growth of breast cancer cells (BCCs). This effect was evaluated through measured cell cycle arrest plus apoptosis through flowcytometry analyses associated with a protein level...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Pharmacological Research - Modern Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142522001397 |
| _version_ | 1811178100165181440 |
|---|---|
| author | Asmaa A. Hassan Mohamed K. Abdel-Rafei Noheir H. Sherif Mostafa A. Askar Noura M Thabet |
| author_facet | Asmaa A. Hassan Mohamed K. Abdel-Rafei Noheir H. Sherif Mostafa A. Askar Noura M Thabet |
| author_sort | Asmaa A. Hassan |
| collection | DOAJ |
| description | This study aimed to observe the anticancer, as well as radiosensitizing, effect of Anagallis arvensis extract (AAE) on the growth of breast cancer cells (BCCs). This effect was evaluated through measured cell cycle arrest plus apoptosis through flowcytometry analyses associated with a protein level of estrogen receptors (ER-α and ER-β), Fork head box O3a (FOXO3), and phosphorylated histone H2AX (γ-H2AX) by western blotting analysis. Further, the gene expression of mir-18, mir-101, mir-421, and let-7 was detected via a real-time polymerase chain reaction (RTPCR). AAE and/or γ-radiation (4Gy) inhibited the mediator-induced growth activation and enhanced radiosensitivity in the BCC lines (MCF-7 and MDA-MB-231). AAE reduced both cell cycle progression and cell growth via induced apoptosis. Additionally, the protein expression of ERα and ERβ was decreased, while the protein expression of FOXO3 and γ-H2AX was increased. Moreover, the gene expression of mir-18 and mir-101 was decreased, while the gene expression of let-7 and mir-421 was stimulated in BCCs. AAE has an anticancer with a radiosensitizing effect and is recommended for BC treatment. |
| first_indexed | 2024-04-11T06:12:39Z |
| format | Article |
| id | doaj.art-0856f8945df24fc2bdf4e94b40dca79d |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-04-11T06:12:39Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-0856f8945df24fc2bdf4e94b40dca79d2022-12-22T04:41:09ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252022-12-015100179Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 ExpressionAsmaa A. Hassan0Mohamed K. Abdel-Rafei1Noheir H. Sherif2Mostafa A. Askar3Noura M Thabet4Radiation Biology Departmentt, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.Radiation Biology Departmentt, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.Drug and Radiation Research Department, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, EgyptRadiation Biology Departmentt, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.; Corresponding authors: Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, 3 Ahmed El-Zomor Street, Egyptian Atomic Energy Authority, P.O. Box 29, Nasr City, Cairo, Egypt. Tel: 202-01005483946.Radiation Biology Departmentt, National Center for Radiation Research and Technology, Egyptian Atomic Energy Authority, Cairo, Egypt.; Corresponding authors: Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, 3 Ahmed El-Zomor Street, Egyptian Atomic Energy Authority, P.O. Box 29, Nasr City, Cairo, Egypt. Tel: 202-01005483946.This study aimed to observe the anticancer, as well as radiosensitizing, effect of Anagallis arvensis extract (AAE) on the growth of breast cancer cells (BCCs). This effect was evaluated through measured cell cycle arrest plus apoptosis through flowcytometry analyses associated with a protein level of estrogen receptors (ER-α and ER-β), Fork head box O3a (FOXO3), and phosphorylated histone H2AX (γ-H2AX) by western blotting analysis. Further, the gene expression of mir-18, mir-101, mir-421, and let-7 was detected via a real-time polymerase chain reaction (RTPCR). AAE and/or γ-radiation (4Gy) inhibited the mediator-induced growth activation and enhanced radiosensitivity in the BCC lines (MCF-7 and MDA-MB-231). AAE reduced both cell cycle progression and cell growth via induced apoptosis. Additionally, the protein expression of ERα and ERβ was decreased, while the protein expression of FOXO3 and γ-H2AX was increased. Moreover, the gene expression of mir-18 and mir-101 was decreased, while the gene expression of let-7 and mir-421 was stimulated in BCCs. AAE has an anticancer with a radiosensitizing effect and is recommended for BC treatment.http://www.sciencedirect.com/science/article/pii/S2667142522001397Breast cancerAnagallis arvensisγ-radiationERsFOXO3Let-7 |
| spellingShingle | Asmaa A. Hassan Mohamed K. Abdel-Rafei Noheir H. Sherif Mostafa A. Askar Noura M Thabet Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression Pharmacological Research - Modern Chinese Medicine Breast cancer Anagallis arvensis γ-radiation ERs FOXO3 Let-7 |
| title | Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression |
| title_full | Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression |
| title_fullStr | Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression |
| title_full_unstemmed | Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression |
| title_short | Antitumor and radiosensitizing effects of Anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating FOXO3, Let-7, and mir-421 Expression |
| title_sort | antitumor and radiosensitizing effects of anagallis arvensis hydromethanolic extract on breast cancer cells through upregulating foxo3 let 7 and mir 421 expression |
| topic | Breast cancer Anagallis arvensis γ-radiation ERs FOXO3 Let-7 |
| url | http://www.sciencedirect.com/science/article/pii/S2667142522001397 |
| work_keys_str_mv | AT asmaaahassan antitumorandradiosensitizingeffectsofanagallisarvensishydromethanolicextractonbreastcancercellsthroughupregulatingfoxo3let7andmir421expression AT mohamedkabdelrafei antitumorandradiosensitizingeffectsofanagallisarvensishydromethanolicextractonbreastcancercellsthroughupregulatingfoxo3let7andmir421expression AT noheirhsherif antitumorandradiosensitizingeffectsofanagallisarvensishydromethanolicextractonbreastcancercellsthroughupregulatingfoxo3let7andmir421expression AT mostafaaaskar antitumorandradiosensitizingeffectsofanagallisarvensishydromethanolicextractonbreastcancercellsthroughupregulatingfoxo3let7andmir421expression AT nouramthabet antitumorandradiosensitizingeffectsofanagallisarvensishydromethanolicextractonbreastcancercellsthroughupregulatingfoxo3let7andmir421expression |