Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV
Between 2015 and 2018, we identified the presence of three so-far-unknown Bluetongue virus (BTV) strains (BTV-MNG1/2018, BTV-MNG2/2016, and BTV-MNG3/2016) circulating in clinical healthy sheep and goats in Mongolia. Virus isolation from EDTA blood samples of BTV-MNG1/2018 and BTV-MNG3/2016 was succe...
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MDPI AG
2020-12-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/1/42 |
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| author | Christina Ries Tumenjargal Sharav Erdene-Ochir Tseren-Ochir Martin Beer Bernd Hoffmann |
| author_facet | Christina Ries Tumenjargal Sharav Erdene-Ochir Tseren-Ochir Martin Beer Bernd Hoffmann |
| author_sort | Christina Ries |
| collection | DOAJ |
| description | Between 2015 and 2018, we identified the presence of three so-far-unknown Bluetongue virus (BTV) strains (BTV-MNG1/2018, BTV-MNG2/2016, and BTV-MNG3/2016) circulating in clinical healthy sheep and goats in Mongolia. Virus isolation from EDTA blood samples of BTV-MNG1/2018 and BTV-MNG3/2016 was successful on the mammalian cell line BSR using blood collected from surveillance. After experimental inoculation of goats with BTV-MNG2/2016 positive blood as inoculum, we observed viraemia in one goat and with the EDTA blood of the experimental inoculation, the propagation of BTV-MNG2/2016 in cell culture was successful on mammalian cell line BSR as well. However, virus isolation experiments for BTV-MNG2/2016 on KC cells were unsuccessful. Furthermore, we generated the complete coding sequence of all three novel Mongolian strains. For atypical BTV, serotyping via the traditional serum neutralization assay is not trivial. We therefore sorted the ‘putative novel atypical serotypes’ according to their segment-2 sequence identities and their time point of sampling. Hence, the BTV-MNG1/2018 isolate forms the ‘putative novel atypical serotype’ 33, the BTV-MNG3/2016 the ‘putative novel atypical serotype’ 35, whereas the BTV-MNG2/2016 strain belongs to the same putative novel atypical serotype ‘30’ as BTV-XJ1407 from China. |
| first_indexed | 2024-03-10T13:41:15Z |
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| id | doaj.art-085b21472938458fbc31e4b338694100 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T13:41:15Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-085b21472938458fbc31e4b3386941002023-11-21T03:00:24ZengMDPI AGViruses1999-49152020-12-011314210.3390/v13010042Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTVChristina Ries0Tumenjargal Sharav1Erdene-Ochir Tseren-Ochir2Martin Beer3Bernd Hoffmann4Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17943 Greifswald-Insel Riems, GermanySchool of Veterinary Medicine, Mongolian University of Life Sciences, Khan-uul District, Zaisan 17024, MongoliaSchool of Veterinary Medicine, Mongolian University of Life Sciences, Khan-uul District, Zaisan 17024, MongoliaFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17943 Greifswald-Insel Riems, GermanyFriedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17943 Greifswald-Insel Riems, GermanyBetween 2015 and 2018, we identified the presence of three so-far-unknown Bluetongue virus (BTV) strains (BTV-MNG1/2018, BTV-MNG2/2016, and BTV-MNG3/2016) circulating in clinical healthy sheep and goats in Mongolia. Virus isolation from EDTA blood samples of BTV-MNG1/2018 and BTV-MNG3/2016 was successful on the mammalian cell line BSR using blood collected from surveillance. After experimental inoculation of goats with BTV-MNG2/2016 positive blood as inoculum, we observed viraemia in one goat and with the EDTA blood of the experimental inoculation, the propagation of BTV-MNG2/2016 in cell culture was successful on mammalian cell line BSR as well. However, virus isolation experiments for BTV-MNG2/2016 on KC cells were unsuccessful. Furthermore, we generated the complete coding sequence of all three novel Mongolian strains. For atypical BTV, serotyping via the traditional serum neutralization assay is not trivial. We therefore sorted the ‘putative novel atypical serotypes’ according to their segment-2 sequence identities and their time point of sampling. Hence, the BTV-MNG1/2018 isolate forms the ‘putative novel atypical serotype’ 33, the BTV-MNG3/2016 the ‘putative novel atypical serotype’ 35, whereas the BTV-MNG2/2016 strain belongs to the same putative novel atypical serotype ‘30’ as BTV-XJ1407 from China.https://www.mdpi.com/1999-4915/13/1/42atypical BTVputative novel serotypesBTV in MongoliaBTV in sheep and goatsBTV |
| spellingShingle | Christina Ries Tumenjargal Sharav Erdene-Ochir Tseren-Ochir Martin Beer Bernd Hoffmann Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV Viruses atypical BTV putative novel serotypes BTV in Mongolia BTV in sheep and goats BTV |
| title | Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV |
| title_full | Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV |
| title_fullStr | Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV |
| title_full_unstemmed | Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV |
| title_short | Putative Novel Serotypes ‘33’ and ‘35’ in Clinically Healthy Small Ruminants in Mongolia Expand the Group of Atypical BTV |
| title_sort | putative novel serotypes 33 and 35 in clinically healthy small ruminants in mongolia expand the group of atypical btv |
| topic | atypical BTV putative novel serotypes BTV in Mongolia BTV in sheep and goats BTV |
| url | https://www.mdpi.com/1999-4915/13/1/42 |
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