Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions
Abstract Background Meniscal injuries are a risk factor for osteoarthritis (OA). While a mechanical pathway between meniscal injury and OA has been described, the biological effects of inflammation on this pathway have yet to be clarified. The aim of our study was to compare levels of specific infla...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Experimental Orthopaedics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40634-016-0041-9 |
| _version_ | 1797321334633529344 |
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| author | Takahiro Ogura Miyako Suzuki Yoshihiro Sakuma Kazuyo Yamauchi Sumihisa Orita Masayuki Miyagi Tetsuhiro Ishikawa Hiroto Kamoda Yasuhiro Oikawa Izumi Kanisawa Kenji Takahashi Hiroki Sakai Tomonori Nagamine Hideaki Fukuda Kazuhisa Takahashi Seiji Ohtori Akihiro Tsuchiya |
| author_facet | Takahiro Ogura Miyako Suzuki Yoshihiro Sakuma Kazuyo Yamauchi Sumihisa Orita Masayuki Miyagi Tetsuhiro Ishikawa Hiroto Kamoda Yasuhiro Oikawa Izumi Kanisawa Kenji Takahashi Hiroki Sakai Tomonori Nagamine Hideaki Fukuda Kazuhisa Takahashi Seiji Ohtori Akihiro Tsuchiya |
| author_sort | Takahiro Ogura |
| collection | DOAJ |
| description | Abstract Background Meniscal injuries are a risk factor for osteoarthritis (OA). While a mechanical pathway between meniscal injury and OA has been described, the biological effects of inflammation on this pathway have yet to be clarified. The aim of our study was to compare levels of specific inflammatory mediators, tumor necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6), and nerve growth factor (NGF), in injured and uninjured meniscal tissue and related knee joint synovium. Methods Tissue samples were obtained from 19 patients, 31.1 ± 13.6 years old, who underwent arthroscopic partial meniscectomy. For analysis, tissue samples were categorized into the following groups: injured meniscal site (IM), non‐injured meniscal site (NIM), synovium ‘nearest’ the lesion (NS), and synovium from the opposite knee compartment, ‘farthest’ synovium (FS). Levels of inflammatory mediators were determined using enzyme‐linked immunosorbent assay and between‐group differences (IM and NIM; NS and FS) were evaluated using the Wilcoxon signed‐rank test. The association between pre‐operative pain score and the level of each inflammatory mediator was evaluated using Spearman’s correlation. Results Higher levels of TNF‐α and IL‐6 were identified in the IM tissue, compared to NIM (p <0.05). IL‐6 levels were also higher in the NS compared to the FS (p <0.05). There was no correlation between pre‐operative pain score and level of each inflammatory mediator. Conclusions Our outcomes confirm a local increase in inflammatory mediator levels, in both meniscal and synovial tissue, which could contribute to development of OA. Management of these biological effects of meniscal injury might be warranted. |
| first_indexed | 2024-03-08T04:57:05Z |
| format | Article |
| id | doaj.art-085c42181c714116b427ebfd51f3cd32 |
| institution | Directory Open Access Journal |
| issn | 2197-1153 |
| language | English |
| last_indexed | 2024-03-08T04:57:05Z |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Experimental Orthopaedics |
| spelling | doaj.art-085c42181c714116b427ebfd51f3cd322024-02-07T15:05:53ZengWileyJournal of Experimental Orthopaedics2197-11532016-01-0131n/an/a10.1186/s40634-016-0041-9Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesionsTakahiro Ogura0Miyako Suzuki1Yoshihiro Sakuma2Kazuyo Yamauchi3Sumihisa Orita4Masayuki Miyagi5Tetsuhiro Ishikawa6Hiroto Kamoda7Yasuhiro Oikawa8Izumi Kanisawa9Kenji Takahashi10Hiroki Sakai11Tomonori Nagamine12Hideaki Fukuda13Kazuhisa Takahashi14Seiji Ohtori15Akihiro Tsuchiya16Funabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanFunabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanFunabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanFunabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanFunabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanFunabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanDepartment of Orthopaedic SurgeryGraduate School of MedicineChiba UniversityChibaJapanFunabashi Orthopaedic Hospital Sports Medicine CenterFunabashiChibaJapanAbstract Background Meniscal injuries are a risk factor for osteoarthritis (OA). While a mechanical pathway between meniscal injury and OA has been described, the biological effects of inflammation on this pathway have yet to be clarified. The aim of our study was to compare levels of specific inflammatory mediators, tumor necrosis factor‐alpha (TNF‐α), interleukin‐6 (IL‐6), and nerve growth factor (NGF), in injured and uninjured meniscal tissue and related knee joint synovium. Methods Tissue samples were obtained from 19 patients, 31.1 ± 13.6 years old, who underwent arthroscopic partial meniscectomy. For analysis, tissue samples were categorized into the following groups: injured meniscal site (IM), non‐injured meniscal site (NIM), synovium ‘nearest’ the lesion (NS), and synovium from the opposite knee compartment, ‘farthest’ synovium (FS). Levels of inflammatory mediators were determined using enzyme‐linked immunosorbent assay and between‐group differences (IM and NIM; NS and FS) were evaluated using the Wilcoxon signed‐rank test. The association between pre‐operative pain score and the level of each inflammatory mediator was evaluated using Spearman’s correlation. Results Higher levels of TNF‐α and IL‐6 were identified in the IM tissue, compared to NIM (p <0.05). IL‐6 levels were also higher in the NS compared to the FS (p <0.05). There was no correlation between pre‐operative pain score and level of each inflammatory mediator. Conclusions Our outcomes confirm a local increase in inflammatory mediator levels, in both meniscal and synovial tissue, which could contribute to development of OA. Management of these biological effects of meniscal injury might be warranted.https://doi.org/10.1186/s40634-016-0041-9Meniscal injuryInflammatory mediatorSynoviumOsteoarthritis |
| spellingShingle | Takahiro Ogura Miyako Suzuki Yoshihiro Sakuma Kazuyo Yamauchi Sumihisa Orita Masayuki Miyagi Tetsuhiro Ishikawa Hiroto Kamoda Yasuhiro Oikawa Izumi Kanisawa Kenji Takahashi Hiroki Sakai Tomonori Nagamine Hideaki Fukuda Kazuhisa Takahashi Seiji Ohtori Akihiro Tsuchiya Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions Journal of Experimental Orthopaedics Meniscal injury Inflammatory mediator Synovium Osteoarthritis |
| title | Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions |
| title_full | Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions |
| title_fullStr | Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions |
| title_full_unstemmed | Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions |
| title_short | Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions |
| title_sort | differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions |
| topic | Meniscal injury Inflammatory mediator Synovium Osteoarthritis |
| url | https://doi.org/10.1186/s40634-016-0041-9 |
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