Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep sequencing
Here we describe a method for constructing small RNA libraries for highthroughput sequencing in which we have made a significant improvement to commonly available standard protocols. We added a locked nucleic acid (LNA) oligonucleotide—named dimer eliminator—that is complementary to the adapter-dime...
| Hauptverfasser: | , , , , , , |
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| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Taylor & Francis Group
2010-10-01
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| Schriftenreihe: | BioTechniques |
| Schlagworte: | |
| Online Zugang: | https://www.future-science.com/doi/10.2144/000113516 |